PMID- 27693304 OWN - NLM STAT- MEDLINE DCOM- 20170421 LR - 20220330 IS - 1879-3150 (Electronic) IS - 0041-0101 (Linking) VI - 122 DP - 2016 Nov TI - Evaluation of antimicrobial, cytotoxic, and hemolytic activities from venom of the spider Lasiodora sp. PG - 119-126 LID - S0041-0101(16)30291-4 [pii] LID - 10.1016/j.toxicon.2016.09.019 [doi] AB - This study characterized the protein/peptide profile of venom isolated from the spider Lasiodora sp. (Mygalomorphae, Theraphosidae) found in northeastern Brazil and determined its antimicrobial activity, toxicity against human cells, and hemolytic activity. Protein concentration of the Lasiodora sp. venom was 4.53 +/- 0.38 mg/mL. SDS-PAGE showed proteins with molecular masses up to 75 kDa, some of which contained disulfide bridges. RP-HPLC analysis separate at least 12 peaks that were identified by mass spectrometry as peptides U(1)-theraphotoxin-Lp1a (lasiotoxin-1), U(1)-theraphotoxin-Lp1c (lasiotoxin-3), U(3)-theraphotoxin-Lsp1a (LTx5), and omega-theraphotoxin-Asp3a as well as the proteins phospholipase A(2) (PLA(2)) and hyaluronidase. The crude venom exhibited bactericidal effect against Aeromonas sp., Bacillus subtilis, and Micrococcus luteus and fungicidal effect against Candida parapsilosis and Candida albicans. In addition, the venom exerted bacteriostatic effect against Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus and fungistatic effect against Candida tropicalis and Candida krusei. The minimum inhibitory (MIC), minimum bactericidal (MBC), and minimum fungicidal (MFC) concentrations ranged from 3.9 to 500 mug/mL. The Lasiodora sp. venom decreased the viability of human peripheral blood mononuclear cells (PBMCs) by 50%-90% at concentrations of 0.1, 1, 10, and 100 mug/mL, promoting apoptosis of these cells. On the other hand, the venom showed weak hemolytic activity against Mus musculus erythrocytes (EC(50): 757 mug/mL). In conclusion, the Lasiodora sp. spider venom is a rich source of antimicrobial agents. Future studies will focus on identifying antimicrobial agents present in this venom and evaluating whether these agents contribute to its cytotoxic effects against PBMCs. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Ferreira, Felipe Roberto Borba AU - Ferreira FR AD - Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - da Silva, Pollyanna Michelle AU - da Silva PM AD - Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - Soares, Tatiana AU - Soares T AD - Centro de Tecnologias Estrategicas do Nordeste, Recife, PE, Brazil. FAU - Goncalves Machado, Larissa AU - Goncalves Machado L AD - Instituto de Bioquimica Medica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. FAU - de Araujo, Larissa Cardoso Correa AU - de Araujo LC AD - Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil; Departamento de Antibioticos, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - da Silva, Teresinha Goncalves AU - da Silva TG AD - Departamento de Antibioticos, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - de Mello, Gabriela Souto Vieira AU - de Mello GS AD - Laboratorio de Imunomodulacao e Novas Abordagens Terapeuticas, Nucleo de Pesquisa em Inovacao Terapeutica (NUPIT), Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - Galdino da Rocha Pitta, Maira AU - Galdino da Rocha Pitta M AD - Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil; Laboratorio de Imunomodulacao e Novas Abordagens Terapeuticas, Nucleo de Pesquisa em Inovacao Terapeutica (NUPIT), Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - de Melo Rego, Moacyr Jesus Barreto AU - de Melo Rego MJ AD - Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil; Laboratorio de Imunomodulacao e Novas Abordagens Terapeuticas, Nucleo de Pesquisa em Inovacao Terapeutica (NUPIT), Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - Pontual, Emmanuel Viana AU - Pontual EV AD - Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil. FAU - Zingali, Russolina Benedeta AU - Zingali RB AD - Instituto de Bioquimica Medica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. FAU - Napoleao, Thiago Henrique AU - Napoleao TH AD - Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil. FAU - Paiva, Patricia Maria Guedes AU - Paiva PM AD - Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, PE, Brazil. Electronic address: ppaivaufpe@yahoo.com.br. LA - eng PT - Journal Article DEP - 20160929 PL - England TA - Toxicon JT - Toxicon : official journal of the International Society on Toxinology JID - 1307333 RN - 0 (Anti-Infective Agents) RN - 0 (Antineoplastic Agents) RN - 0 (Spider Venoms) SB - IM MH - Animals MH - Anti-Infective Agents/*pharmacology MH - Antineoplastic Agents/*pharmacology MH - Hemolysis/*drug effects MH - Humans MH - Infant MH - Spider Venoms/*chemistry OTO - NOTNLM OT - Antibacterial OT - Antifungal OT - Apoptosis OT - Lasiodora OT - Spider venom EDAT- 2016/10/30 06:00 MHDA- 2017/04/22 06:00 CRDT- 2016/10/04 06:00 PHST- 2016/05/02 00:00 [received] PHST- 2016/09/07 00:00 [revised] PHST- 2016/09/28 00:00 [accepted] PHST- 2016/10/30 06:00 [pubmed] PHST- 2017/04/22 06:00 [medline] PHST- 2016/10/04 06:00 [entrez] AID - S0041-0101(16)30291-4 [pii] AID - 10.1016/j.toxicon.2016.09.019 [doi] PST - ppublish SO - Toxicon. 2016 Nov;122:119-126. doi: 10.1016/j.toxicon.2016.09.019. Epub 2016 Sep 29.