PMID- 27695957
OWN - NLM
STAT- MEDLINE
DCOM- 20170127
LR  - 20180928
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 162
IP  - 1
DP  - 2017 Jan
TI  - Humoral immune responses and viral shedding following vaccination with modified 
      live porcine reproductive and respiratory syndrome virus vaccines.
PG  - 139-146
LID - 10.1007/s00705-016-3084-4 [doi]
AB  - The antibody response and pattern of shedding of vaccine virus following 
      vaccination with modified live genotype I or II porcine reproductive and 
      respiratory syndrome virus (PRRSV) vaccines (MLVs) were investigated. Ninety 
      PRRSV-free pigs were divided randomly seven, groups including the NEG, EU1, EU2, 
      US1, US2, US3 and US4 groups. The NEG group was unvaccinated. The EU1, EU2, US1, 
      US2, US3 and US4 groups were vaccinated with the following MLVs: AMERVAC((R)) PRRS, 
      Porcillis((R)) PRRS, Fostera PRRS, Ingelvac((R)) PRRS MLV, Ingelvac((R)) PRRS ATP, and 
      PrimePac PRRS+ , respectively. Sera were quantitatively assayed for viral RNA 
      using qPCR. Antibody responses were measured using Idexx ELISA and serum 
      neutralization (SN). Shedding of vaccine virus was investigated using sentinel 
      pigs and by detection of viral RNA in tonsil scrapings. Antibody responses were 
      detected by ELISA at 7-14 days post-vaccination (DPV) and persisted at high 
      titers until 84 DPV in all MLV groups. The SN titers were delayed and 
      isolate-specific. SN titers were higher for the homologous virus than for 
      heterologous viruses. Age-matched sentinel pigs introduced into the EU2, US2 and 
      US3 groups at 60 DPV seroconverted. In contrast, sentinel pigs introduced at 84 
      DPV remained negative in all of the MLV groups. Vaccine viral RNA was detected in 
      tonsil scrapings from the EU2, US2 and US3 groups at 84-90 DPV. No viral RNA was 
      detected beyond 70 DPV in the EU1, US1 and US4 groups. In conclusion, all MLV 
      genotypes induced rapid antibody responses, which were measured using ELISA. The 
      development of SN antibodies was delayed and isolate-specific. However, the 
      shedding pattern was variable and depended on the by virus isolate used to 
      manufacture the vaccine.
FAU - Madapong, Adthakorn
AU  - Madapong A
AD  - Department of Veterinary Microbiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Bangkok, 10330, Thailand.
FAU - Temeeyasen, Gun
AU  - Temeeyasen G
AD  - Department of Veterinary Microbiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Bangkok, 10330, Thailand.
FAU - Saeng-Chuto, Kepalee
AU  - Saeng-Chuto K
AD  - Department of Veterinary Microbiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Bangkok, 10330, Thailand.
FAU - Tripipat, Thitima
AU  - Tripipat T
AD  - Department of Veterinary Microbiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Bangkok, 10330, Thailand.
FAU - Navasakuljinda, Wichian
AU  - Navasakuljinda W
AD  - Zoetis (Thailand) Limited, Bangkok, Thailand.
FAU - Boonsoongnern, Alongkot
AU  - Boonsoongnern A
AD  - Department of Farm Resources and Production Medicine, Faculty of Veterinary 
      Medicine Kamphaeng Saen Campus, Kasetsart University, Nakhon Pathom, Thailand.
FAU - Tantituvanont, Angkana
AU  - Tantituvanont A
AD  - Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical 
      Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
FAU - Nilubol, Dachrit
AU  - Nilubol D
AUID- ORCID: 0000-0002-7691-6790
AD  - Department of Veterinary Microbiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Bangkok, 10330, Thailand. dachrit@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160930
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Cohort Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Male
MH  - Neutralization Tests
MH  - Palatine Tonsil/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/*isolation & 
      purification
MH  - RNA, Viral/analysis/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/immunology
MH  - Viral Vaccines/*administration & dosage/*immunology
MH  - *Virus Shedding
EDAT- 2016/10/04 06:00
MHDA- 2017/01/28 06:00
CRDT- 2016/10/04 06:00
PHST- 2016/08/02 00:00 [received]
PHST- 2016/09/20 00:00 [accepted]
PHST- 2016/10/04 06:00 [pubmed]
PHST- 2017/01/28 06:00 [medline]
PHST- 2016/10/04 06:00 [entrez]
AID - 10.1007/s00705-016-3084-4 [pii]
AID - 10.1007/s00705-016-3084-4 [doi]
PST - ppublish
SO  - Arch Virol. 2017 Jan;162(1):139-146. doi: 10.1007/s00705-016-3084-4. Epub 2016 
      Sep 30.