PMID- 27698849
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 12
IP  - 4
DP  - 2016 Oct
TI  - Fluorescence in situ hybridization testing of chromosomes 6, 8, 9 and 11 in 
      melanocytic tumors is difficult to automate and reveals tumor heterogeneity in 
      melanomas.
PG  - 2734-2741
AB  - Malignant melanomas may be difficult to differentiate from benign nevi on the 
      basis of histology. Contrary to nevi, the majority of melanomas harbor 
      chromosomal imbalances. Comparative genomic hybridization-based and fluorescence 
      in situ hybridization (FISH) tests can help differentiating malignant from benign 
      tumors. In the present study, eight-bacterial artificial chromosome (BAC) probes 
      targeting chromosomes 6, 8, 9 and 11 were tested by FISH, and compared with a 
      commercial four-color FISH probe set targeting chromosomes 6 and 11 in a first 
      set of 62 tissue microarray-included melanocytic tumors (47 melanomas and 15 
      nevi). A second set of 108 tumors (70 melanomas and 38 nevi) was analyzed with 
      the eight-probes kit, and manual counting was compared with the newly developed 
      automated FISH signals counting and with semi-quantitative visual detection of 
      chromosomal imbalances. Intra-tumor heterogeneity was also evaluated in 12 
      melanomas and 10 patients with paired melanoma samples. Testing the tumors from 
      the first set with the commercial kit and the eight-probes test permitted to 
      correctly identify 45/47 and 47/47 melanomas, respectively. In the second tumor 
      set, 65/70 malignant tumors presented at least one chromosomal imbalance, whereas 
      none was detected in the nevi. The agreement between manual and automated signals 
      counting was better in good-quality FISH slides compared with poor-quality 
      slides. Semi-quantitative visual appreciation of chromosomal imbalances also 
      reached strong agreement with exact manual counting. In addition, a frequent 
      cytogenetic heterogeneity within melanomas and between paired tumors was noticed 
      in patients with metastatic melanomas. To conclude, FISH testing targeting 
      chromosomes 6, 8, 9 and 11 enabled to differentiate the majority of melanomas 
      from nevi but was difficult to automate. Tumor cytogenetic heterogeneity was 
      frequent and could impair FISH testing.
FAU - Uguen, Arnaud
AU  - Uguen A
AD  - Inserm, U1078, F-29609 Brest, France; Department of Pathology, Brest University 
      Hospital, F-29220 Brest, France; European University of Brittany, F-29220 Brest, 
      France.
FAU - Uguen, Marie
AU  - Uguen M
AD  - Department of Pathology, Brest University Hospital, F-29220 Brest, France.
FAU - Talagas, Matthieu
AU  - Talagas M
AD  - Department of Pathology, Brest University Hospital, F-29220 Brest, France; 
      European University of Brittany, F-29220 Brest, France; EA4685, Brest Medical 
      School, F-29220 Brest, France.
FAU - Gobin, Eric
AU  - Gobin E
AD  - Department of Pathology, Brest University Hospital, F-29220 Brest, France.
FAU - Marcorelles, Pascale
AU  - Marcorelles P
AD  - Department of Pathology, Brest University Hospital, F-29220 Brest, France; 
      European University of Brittany, F-29220 Brest, France; EA4685, Brest Medical 
      School, F-29220 Brest, France.
FAU - De Braekeleer, Marc
AU  - De Braekeleer M
AD  - Inserm, U1078, F-29609 Brest, France; European University of Brittany, F-29220 
      Brest, France; Department of Cytogenetics and Reproduction Biology, Brest 
      University Hospital, F-29220 Brest, France.
LA  - eng
PT  - Journal Article
DEP - 20160803
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5038458
OTO - NOTNLM
OT  - chromosome
OT  - fluorescence in situ hybridization
OT  - melanoma
OT  - nevus
OT  - tumor heterogeneity
EDAT- 2016/10/05 06:00
MHDA- 2016/10/05 06:01
PMCR- 2016/08/03
CRDT- 2016/10/05 06:00
PHST- 2015/11/22 00:00 [received]
PHST- 2016/06/16 00:00 [accepted]
PHST- 2016/10/05 06:00 [entrez]
PHST- 2016/10/05 06:00 [pubmed]
PHST- 2016/10/05 06:01 [medline]
PHST- 2016/08/03 00:00 [pmc-release]
AID - OL-0-0-4949 [pii]
AID - 10.3892/ol.2016.4949 [doi]
PST - ppublish
SO  - Oncol Lett. 2016 Oct;12(4):2734-2741. doi: 10.3892/ol.2016.4949. Epub 2016 Aug 3.