PMID- 27708366
OWN - NLM
STAT- MEDLINE
DCOM- 20180618
LR  - 20181202
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Oct 6
TI  - Biotransformation of ferulic acid to vanillin in the packed bed-stirred 
      fermentors.
PG  - 34644
LID - 10.1038/srep34644 [doi]
LID - 34644
AB  - We performed the biotransformation of ferulic acid to vanillin using Bacillus 
      subtilis (B. subtilis) in the stirring packed-bed reactors filled with carbon 
      fiber textiles (CFT). Scanning electron microscope (SEM), HPLC, qRT-PCR and ATP 
      assay indicated that vanillin biotransformation is tightly related to cell 
      growth, cellar activity and the extent of biofilm formation. The 
      biotransformation was affected by hydraulic retention time (HRT), temperature, 
      initial pH, stirring speed and ferulic acid concentration, and the maximum 
      vanillin production was obtained at 20 h, 35  degrees C, 9.0, 200 rpm, 1.5 g/L, 
      respectively. Repeated batch biotransformation performed under this optimized 
      condition showed that the maximum productivity (0.047 g/L/h) and molar yield 
      (60.43%) achieved in immobilized cell system were 1.84 and 3.61 folds higher than 
      those achieved in free cell system. Therefore, the stirring reactor packed with 
      CFT carrier biofilm formed by B. subtilis represented a valid biocatalytic system 
      for the production of vanillin.
FAU - Yan, Lei
AU  - Yan L
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Xinfeng Road No.5, Daqing, 163319, P.R. China.
FAU - Chen, Peng
AU  - Chen P
AD  - School of Pharmacy, Lanzhou University, Donggang West Road No. 199, Lanzhou, 
      730020, P.R. China.
FAU - Zhang, Shuang
AU  - Zhang S
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Xinfeng Road No.5, Daqing, 163319, P.R. China.
FAU - Li, Suyue
AU  - Li S
AD  - Gansu Institute of Business and Technology, Yannan Road No. 449, Lanzhou, 730010, 
      P.R. China.
FAU - Yan, Xiaojuan
AU  - Yan X
AD  - Gansu Institute of Business and Technology, Yannan Road No. 449, Lanzhou, 730010, 
      P.R. China.
FAU - Wang, Ningbo
AU  - Wang N
AD  - Gansu Institute of Business and Technology, Yannan Road No. 449, Lanzhou, 730010, 
      P.R. China.
FAU - Liang, Ning
AU  - Liang N
AD  - Gansu Institute of Business and Technology, Yannan Road No. 449, Lanzhou, 730010, 
      P.R. China.
FAU - Li, Hongyu
AU  - Li H
AD  - School of Pharmacy, Lanzhou University, Donggang West Road No. 199, Lanzhou, 
      730020, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161006
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Benzaldehydes)
RN  - 0 (Carbon Fiber)
RN  - 0 (Coumaric Acids)
RN  - 7440-44-0 (Carbon)
RN  - AVM951ZWST (ferulic acid)
RN  - CHI530446X (vanillin)
SB  - IM
MH  - Bacillus subtilis/*metabolism
MH  - Batch Cell Culture Techniques/methods
MH  - Benzaldehydes/*metabolism
MH  - Biofilms/*growth & development
MH  - Bioreactors
MH  - Biotransformation
MH  - Carbon/chemistry
MH  - Carbon Fiber
MH  - Cells, Immobilized/*metabolism
MH  - Coumaric Acids/*metabolism
MH  - Fermentation
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Temperature
MH  - Textiles
PMC - PMC5052561
EDAT- 2016/10/07 06:00
MHDA- 2018/06/19 06:00
PMCR- 2016/10/06
CRDT- 2016/10/07 06:00
PHST- 2016/01/28 00:00 [received]
PHST- 2016/09/14 00:00 [accepted]
PHST- 2016/10/07 06:00 [entrez]
PHST- 2016/10/07 06:00 [pubmed]
PHST- 2018/06/19 06:00 [medline]
PHST- 2016/10/06 00:00 [pmc-release]
AID - srep34644 [pii]
AID - 10.1038/srep34644 [doi]
PST - epublish
SO  - Sci Rep. 2016 Oct 6;6:34644. doi: 10.1038/srep34644.