PMID- 27708635
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 7
DP  - 2016
TI  - The Kinome of Edible and Medicinal Fungus Wolfiporia cocos.
PG  - 1495
LID - 1495
AB  - Wolfiporia cocos is an edible and medicinal fungus that grows in association with 
      pine trees, and its dried sclerotium, known as Fuling in China, has been used as 
      a traditional medicine in East Asian countries for centuries. Nearly 10% of the 
      traditional Chinese medicinal preparations contain W. cocos. Currently, the 
      commercial production of Fuling is limited because of the lack of pine-based 
      substrate and paucity of knowledge about the sclerotial development of the 
      fungus. Since protein kinase (PKs) play significant roles in the regulation of 
      growth, development, reproduction, and environmental responses in filamentous 
      fungi, the kinome of W. cocos was analyzed by identifying the PKs genes, studying 
      transcript profiles and assigning PKs to orthologous groups. Of the 10 putative 
      PKs, 11 encode atypical PKs, and 13, 10, 2, 22, and 11 could encoded PKs from the 
      AGC, CAMK, CK, CMGC, STE, and TLK Groups, respectively. The level of transcripts 
      from PK genes associated with sclerotia formation in the mycelium and sclerotium 
      stages were analyzed by qRT-PCR. Based on the functions of the orthologs in 
      Sclerotinia sclerotiorum (a sclerotia-formation fungus) and Saccharomyces 
      cerevisiae, the potential roles of these W. cocos PKs were assigned. To the best 
      of our knowledge, our study is the first identification and functional discussion 
      of the kinome in the edible and medicinal fungus W. cocos. Our study 
      systematically suggests potential roles of W. cocos PKs and provide comprehensive 
      and novel insights into W. cocos sclerotial development and other economically 
      important traits. Additionally, based on our result, genetic engineering can be 
      employed for over expression or interference of some significant PKs genes to 
      promote sclerotial growth and the accumulation of active compounds.
FAU - Wei, Wei
AU  - Wei W
AD  - Institute for Interdisciplinary Research, Jianghan University Wuhan, China.
FAU - Shu, Shaohua
AU  - Shu S
AD  - College of Plant Science and Technology, Huazhong Agricultural University Wuhan, 
      China.
FAU - Zhu, Wenjun
AU  - Zhu W
AD  - College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University 
      Wuhan, China.
FAU - Xiong, Ying
AU  - Xiong Y
AD  - Hefei Inzyme Information Technology Co., Ltd Wuhan, China.
FAU - Peng, Fang
AU  - Peng F
AD  - College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University 
      Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20160921
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5030230
OTO - NOTNLM
OT  - Wolfiporia cocos
OT  - edible and medicinal fungus
OT  - kinome
OT  - protein kinase (PKs)
OT  - sclerotial development
EDAT- 2016/10/07 06:00
MHDA- 2016/10/07 06:01
PMCR- 2016/09/21
CRDT- 2016/10/07 06:00
PHST- 2016/08/13 00:00 [received]
PHST- 2016/09/07 00:00 [accepted]
PHST- 2016/10/07 06:00 [entrez]
PHST- 2016/10/07 06:00 [pubmed]
PHST- 2016/10/07 06:01 [medline]
PHST- 2016/09/21 00:00 [pmc-release]
AID - 10.3389/fmicb.2016.01495 [doi]
PST - epublish
SO  - Front Microbiol. 2016 Sep 21;7:1495. doi: 10.3389/fmicb.2016.01495. eCollection 
      2016.