PMID- 27709890
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20181106
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 8
IP  - 43
DP  - 2016 Nov 2
TI  - A Biodegradable Polycationic Paint that Kills Bacteria in Vitro and in Vivo.
PG  - 29298-29309
AB  - Bacterial colonization and subsequent formation of biofilms onto surfaces of 
      medical devices and implants is a major source of nosocomial infections. Most 
      antibacterial coatings to combat infections are either metal-based or 
      nondegradable-polymer-based and hence limited by their nondegradability and 
      unpredictable toxicity. Moreover, to combat infections effectively, the coatings 
      are required to display simultaneous antibacterial and antibiofilm activity. 
      Herein we report biocompatible and biodegradable coatings based on organo-soluble 
      quaternary chitin polymers which were immobilized noncovalently onto surfaces as 
      bactericidal paint. The polycationic paint was found to be active against both 
      drug-sensitive and -resistant bacteria such as methicillin-resistant 
      Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), 
      and beta-lactam-resistant Klebsiella pneumoniae. The cationic polymers were shown to 
      interact with the negatively charged bacterial cell membrane and disrupt the 
      membrane integrity, thereby causing leakage of intracellular constituents and 
      cell death upon contact. Importantly, surfaces coated with the polymers inhibited 
      formation of biofilms against both Gram-positive S. aureus and Gram-negative E. 
      coli, two of the most clinically important bacteria that form biofilms. Surfaces 
      coated with the polymers displayed negligible toxicity against human erythrocytes 
      and embryo kidney cells. Notably, the polymers were shown to be susceptible 
      toward lysozyme. Furthermore, subcutaneous implantation of polymer discs in rats 
      led to 15-20% degradation in 4 weeks thereby displaying their biodegradability. 
      In a murine model of subcutaneous infection, polymer-coated medical-grade 
      catheter reduced MRSA burden by 3.7 log compared to that of noncoated catheter. 
      Furthermore, no biofilm development was observed on the coated catheters under in 
      vivo conditions. The polycationic materials thus developed herein represent a 
      novel class of safe and effective coating agents for the prevention of 
      device-associated infections.
FAU - Hoque, Jiaul
AU  - Hoque J
AD  - Chemical Biology and Medicinal Chemistry Laboratory, New Chemistry Unit, 
      Jawaharlal Nehru Centre for Advanced Scientific Research , Jakkur, Bengaluru 
      560064, India.
FAU - Akkapeddi, Padma
AU  - Akkapeddi P
AD  - Chemical Biology and Medicinal Chemistry Laboratory, New Chemistry Unit, 
      Jawaharlal Nehru Centre for Advanced Scientific Research , Jakkur, Bengaluru 
      560064, India.
FAU - Ghosh, Chandradhish
AU  - Ghosh C
AD  - Chemical Biology and Medicinal Chemistry Laboratory, New Chemistry Unit, 
      Jawaharlal Nehru Centre for Advanced Scientific Research , Jakkur, Bengaluru 
      560064, India.
FAU - Uppu, Divakara S S M
AU  - Uppu DS
AD  - Chemical Biology and Medicinal Chemistry Laboratory, New Chemistry Unit, 
      Jawaharlal Nehru Centre for Advanced Scientific Research , Jakkur, Bengaluru 
      560064, India.
FAU - Haldar, Jayanta
AU  - Haldar J
AD  - Chemical Biology and Medicinal Chemistry Laboratory, New Chemistry Unit, 
      Jawaharlal Nehru Centre for Advanced Scientific Research , Jakkur, Bengaluru 
      560064, India.
LA  - eng
PT  - Journal Article
DEP - 20161020
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Biofilms
MH  - Escherichia coli
MH  - Humans
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - *Paint
MH  - Rats
MH  - Staphylococcus aureus
OTO - NOTNLM
OT  - anti-infective biomaterials
OT  - biodegradable bactericidal paint
OT  - biofilm inhibition
OT  - drug-resistant bacteria
OT  - organo-soluble quaternary chitin derivatives
EDAT- 2016/11/03 06:00
MHDA- 2018/11/07 06:00
CRDT- 2016/10/07 06:00
PHST- 2016/11/03 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
PHST- 2016/10/07 06:00 [entrez]
AID - 10.1021/acsami.6b09804 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2016 Nov 2;8(43):29298-29309. doi: 
      10.1021/acsami.6b09804. Epub 2016 Oct 20.