PMID- 27713086 OWN - NLM STAT- MEDLINE DCOM- 20171030 LR - 20180220 IS - 1878-7568 (Electronic) IS - 1742-7061 (Linking) VI - 47 DP - 2017 Jan 1 TI - Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. PG - 50-59 LID - S1742-7061(16)30520-7 [pii] LID - 10.1016/j.actbio.2016.10.002 [doi] AB - The objective of this work was to exploit the fibronectin type II (FNII) module from human matrix metalloproteinase-2 as a functional domain for the development of silk-based biopolymer blends that display enhanced cell adhesion properties. The DNA sequence of spider dragline silk protein (6mer) was genetically fused with the FNII coding sequence and expressed in Escherichia coli. The chimeric protein 6mer+FNII was purified by non-chromatographic methods. Films prepared from 6mer+FNII by solvent casting promoted only limited cell adhesion of human skin fibroblasts. However, the performance of the material in terms of cell adhesion was significantly improved when 6mer+FNII was combined with a silk-elastin-like protein in a concentration-dependent behavior. With this work we describe a novel class of biopolymer that promote cell adhesion and potentially useful as biomaterials for tissue engineering and regenerative medicine. STATEMENT OF SIGNIFICANCE: This work reports the development of biocompatible silk-based composites with enhanced cell adhesion properties suitable for biomedical applications in regenerative medicine. The biocomposites were produced by combining a genetically engineered silk-elastin-like protein with a genetically engineered spider-silk-based polypeptide carrying the three domains of the fibronectin type II module from human metalloproteinase-2. These composites were processed into free-standing films by solvent casting and characterized for their biological behavior. To our knowledge this is the first report of the exploitation of all three FNII domains as a functional domain for the development of bioinspired materials with improved biological performance. The present study highlights the potential of using genetically engineered protein-based composites as a platform for the development of new bioinspired biomaterials. CI - Copyright (c) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. FAU - Pereira, Ana Margarida AU - Pereira AM AD - CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimaraes, Portugal. FAU - Machado, Raul AU - Machado R AD - CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: raulmachado@bio.uminho.pt. FAU - da Costa, Andre AU - da Costa A AD - CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. FAU - Ribeiro, Artur AU - Ribeiro A AD - CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. FAU - Collins, Tony AU - Collins T AD - CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. FAU - Gomes, Andreia C AU - Gomes AC AD - CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. FAU - Leonor, Isabel B AU - Leonor IB AD - 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimaraes, Portugal. FAU - Kaplan, David L AU - Kaplan DL AD - Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. FAU - Reis, Rui L AU - Reis RL AD - 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimaraes, Portugal. FAU - Casal, Margarida AU - Casal M AD - CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: mcasal@bio.uminho.pt. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20161003 PL - England TA - Acta Biomater JT - Acta biomaterialia JID - 101233144 RN - 0 (Biocompatible Materials) RN - 0 (Fibronectins) RN - 0 (Silk) SB - IM EIN - Acta Biomater. 2017 Feb;49:604. PMID: 27843093 MH - Amino Acid Sequence MH - Biocompatible Materials/*pharmacology MH - Blotting, Western MH - Cell Adhesion/drug effects MH - Cell Death/drug effects MH - Cell Line MH - Cell Survival/drug effects MH - Electrophoresis, Polyacrylamide Gel MH - Fibroblasts/*cytology/drug effects MH - Fibronectins/chemistry/isolation & purification/*pharmacology MH - Humans MH - Mechanical Phenomena MH - Silk/*pharmacology MH - Spectroscopy, Fourier Transform Infrared OTO - NOTNLM OT - Biomaterials OT - Cell adhesion OT - Elastin OT - Fibronectin type II OT - Silk EDAT- 2016/10/25 06:00 MHDA- 2017/10/31 06:00 CRDT- 2016/10/08 06:00 PHST- 2016/04/28 00:00 [received] PHST- 2016/09/20 00:00 [revised] PHST- 2016/10/02 00:00 [accepted] PHST- 2016/10/25 06:00 [pubmed] PHST- 2017/10/31 06:00 [medline] PHST- 2016/10/08 06:00 [entrez] AID - S1742-7061(16)30520-7 [pii] AID - 10.1016/j.actbio.2016.10.002 [doi] PST - ppublish SO - Acta Biomater. 2017 Jan 1;47:50-59. doi: 10.1016/j.actbio.2016.10.002. Epub 2016 Oct 3.