PMID- 27714657
OWN - NLM
STAT- MEDLINE
DCOM- 20170424
LR  - 20210109
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 24
IP  - 1
DP  - 2017 Jan
TI  - Accumulation of petroleum hydrocarbons in intracellular lipid bodies of the 
      freshwater diatom Synedra acus subsp. radians.
PG  - 275-283
LID - 10.1007/s11356-016-7782-y [doi]
AB  - The accumulation of hydrophobic compounds by phytoplankton plays a crucial role 
      in the biogeochemical cycle of persistent organic pollutants (POPs) in aquatic 
      environments. We studied the accumulation of polycyclic aromatic hydrocarbons 
      (PAHs) in the freshwater diatom Synedra acus subsp. radians during its 
      cultivation with crude oil hydrocarbons, using epifluorescent and laser confocal 
      microscopy as well as gas chromatography-mass spectrometry (GC/MS) analysis. Our 
      results revealed that in the presence of crude oil or an extract of a crude 
      oil/n-hexane solution (light oil), S. acus subsp. radians accumulated PAHs in its 
      lipid bodies. During cultivation in the presence of a crude oil/n-hexane 
      solution, the cells selectively accumulated C12-C18 alkanes, with a preference 
      for C15 and C16 homologues. The length of n-alkane hydrocarbon chains accumulated 
      in cells was similar to the acyl chains of fatty acids of the diatom. We 
      therefore suggest that the insertion of n-alkanes into the membrane lipid bilayer 
      promotes the transmembrane transport of PAH in diatoms. Our results confirm the 
      hypothesis that diatoms play a role in the elimination of hydrophobic 
      hydrocarbons from aquatic systems.
FAU - Shishlyannikov, Sergey M
AU  - Shishlyannikov SM
AD  - Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 
      Ulan-Batorskaya, 664033, Irkutsk, Russia. sershilin@gmail.com.
FAU - Nikonova, Alyona A
AU  - Nikonova AA
AD  - Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 
      Ulan-Batorskaya, 664033, Irkutsk, Russia.
FAU - Klimenkov, Igor V
AU  - Klimenkov IV
AD  - Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 
      Ulan-Batorskaya, 664033, Irkutsk, Russia.
FAU - Gorshkov, Alexander G
AU  - Gorshkov AG
AD  - Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3 
      Ulan-Batorskaya, 664033, Irkutsk, Russia.
LA  - eng
PT  - Journal Article
DEP - 20161006
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Alkanes)
RN  - 0 (Hydrocarbons)
RN  - 0 (Petroleum)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Alkanes/analysis/metabolism
MH  - Animals
MH  - Diatoms/*chemistry/metabolism
MH  - Fresh Water/analysis
MH  - Gas Chromatography-Mass Spectrometry
MH  - Hydrocarbons/*analysis/metabolism
MH  - Lipid Droplets/*chemistry/metabolism
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - *Models, Theoretical
MH  - Petroleum/*analysis/metabolism
MH  - Phytoplankton/chemistry/metabolism
MH  - Polycyclic Aromatic Hydrocarbons/analysis/metabolism
MH  - Water Pollutants, Chemical/*analysis/metabolism
OTO - NOTNLM
OT  - Accumulation
OT  - Diatom
OT  - Lake Baikal
OT  - Lipid bodies
OT  - PAH
OT  - Synedra acus subsp. radians
EDAT- 2016/10/08 06:00
MHDA- 2017/04/25 06:00
CRDT- 2016/10/08 06:00
PHST- 2016/02/25 00:00 [received]
PHST- 2016/09/26 00:00 [accepted]
PHST- 2016/10/08 06:00 [pubmed]
PHST- 2017/04/25 06:00 [medline]
PHST- 2016/10/08 06:00 [entrez]
AID - 10.1007/s11356-016-7782-y [pii]
AID - 10.1007/s11356-016-7782-y [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2017 Jan;24(1):275-283. doi: 
      10.1007/s11356-016-7782-y. Epub 2016 Oct 6.