PMID- 27714784
OWN - NLM
STAT- MEDLINE
DCOM- 20170606
LR  - 20181202
IS  - 1365-2362 (Electronic)
IS  - 0014-2972 (Linking)
VI  - 47
IP  - 2
DP  - 2017 Feb
TI  - Platelets Toll-like receptor-4 in Crohns disease.
PG  - 109-116
LID - 10.1111/eci.12686 [doi]
AB  - BACKGROUND: Platelets are activated in Crohn's disease (CD) and interplay with 
      leukocytes. Engagement of Toll-like receptor-4 (TLR-4), which is expressed in 
      human platelets, may be involved in crosstalks between platelets and leukocytes 
      leading to their mutual activation for host defense. MATERIALS AND METHODS: Human 
      neutrophil peptides (HNPs), lipoprotein binding peptides, and sCD14 were 
      determined by enzyme-linked immunosorbent assays in 42 patients with active CD, 
      in 43 patients with CD in remission, and in 30 healthy individuals. 
      Neutrophil-platelet aggregates and binding of the TLR-4 monoclonal antibody to 
      platelets were determined by flow cytometry. RESULTS: Levels of HNPs were higher 
      in patients with CD than in controls (P = 0.0003 vs. active CD and P = 0.01 vs. 
      CD in remission). Likewise, neutrophils with adhering platelets were higher in 
      patients with active CD than in controls (P = 0.004). Binding of the TLR-4 
      antibody in patients with active CD was similar to that in controls, while 
      patients in remission had significantly higher binding capacities (P = 0.59 and P 
      = 0.003). Incubation of plasma from patients with active disease or patients in 
      remission with platelets from healthy controls confirmed lower binding of the 
      TLR-4 antibody in the presence of plasma from active diseased patients compared 
      to controls (P = 0.039), possibly due to high levels of lipopolysaccharides, as 
      suggested by high levels of sCD14 and lipoprotein binding protein. CONCLUSION: 
      Our study indicates involvement of platelet TLR-4 in enhancing the secretion of 
      antimicrobial peptides from neutrophils. While platelet aggregation can be due to 
      a variety of mechanisms in inflammatory disease, the mutual activation of 
      platelets and neutrophils may augment host defense.
CI  - (c) 2016 Stichting European Society for Clinical Investigation Journal Foundation.
FAU - Schmid, Werner
AU  - Schmid W
AD  - Division of Cardiothoracic and Vascular Anesthesia and Intensive Care, Department 
      of Anesthesiology, General Intensive Care and Pain Medicine, Medical University 
      of Vienna, Vienna, Austria.
FAU - Novacek, Gottfried
AU  - Novacek G
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine III, 
      Medical University of Vienna, Vienna, Austria.
FAU - Vogelsang, Harald
AU  - Vogelsang H
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine III, 
      Medical University of Vienna, Vienna, Austria.
FAU - Papay, Pavol
AU  - Papay P
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine III, 
      Medical University of Vienna, Vienna, Austria.
FAU - Primas, Christian
AU  - Primas C
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine III, 
      Medical University of Vienna, Vienna, Austria.
FAU - Eser, Alexander
AU  - Eser A
AD  - Division of Gastroenterology and Hepatology, Department of Internal Medicine III, 
      Medical University of Vienna, Vienna, Austria.
FAU - Panzer, Simon
AU  - Panzer S
AD  - Department of Blood Group Serology and Transfusion Medicine, Medical University 
      of Vienna, Vienna, Austria.
LA  - eng
PT  - Journal Article
DEP - 20161229
PL  - England
TA  - Eur J Clin Invest
JT  - European journal of clinical investigation
JID - 0245331
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (alpha-Defensins)
RN  - 0 (lipopolysaccharide-binding protein)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Acute-Phase Proteins/metabolism
MH  - Adult
MH  - Blood Platelets/*metabolism
MH  - C-Reactive Protein/metabolism
MH  - Carrier Proteins/metabolism
MH  - Case-Control Studies
MH  - Cell Communication/physiology
MH  - Crohn Disease/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Male
MH  - Membrane Glycoproteins/metabolism
MH  - Neutrophil Activation/physiology
MH  - Neutrophils/metabolism
MH  - Platelet Activation/physiology
MH  - Platelet Aggregation/physiology
MH  - Toll-Like Receptor 4/*physiology
MH  - alpha-Defensins/*metabolism
OTO - NOTNLM
OT  - Crohn's disease
OT  - Toll-like receptor-4
OT  - human neutrophil alpha-defensins
EDAT- 2016/10/08 06:00
MHDA- 2017/06/07 06:00
CRDT- 2016/10/08 06:00
PHST- 2016/04/10 00:00 [received]
PHST- 2016/10/04 00:00 [accepted]
PHST- 2016/10/08 06:00 [pubmed]
PHST- 2017/06/07 06:00 [medline]
PHST- 2016/10/08 06:00 [entrez]
AID - 10.1111/eci.12686 [doi]
PST - ppublish
SO  - Eur J Clin Invest. 2017 Feb;47(2):109-116. doi: 10.1111/eci.12686. Epub 2016 Dec 
      29.