PMID- 27714813
OWN - NLM
STAT- MEDLINE
DCOM- 20170516
LR  - 20171116
IS  - 2042-7158 (Electronic)
IS  - 0022-3573 (Linking)
VI  - 68
IP  - 12
DP  - 2016 Dec
TI  - Propolis modulates miRNAs involved in TLR-4 pathway, NF-kappaB activation, cytokine 
      production and in the bactericidal activity of human dendritic cells.
PG  - 1604-1612
LID - 10.1111/jphp.12628 [doi]
AB  - OBJECTIVES: Dendritic cells (DCs) are antigen-presenting cells, essential for 
      recognition and presentation of pathogens to T cells. Propolis, a resinous 
      material produced by bees from various plants, exhibits numerous biological 
      properties, highlighting its immunomodulatory action. Here, we assayed the 
      effects of propolis on the maturation and function of human DCs. METHODS: DCs 
      were generated from human monocytes and incubated with propolis and LPS. NF-kappaB 
      and cytokines production were determined by ELISA. microRNA's expression was 
      analysed by RT-qPCR and cell markers detection by flow cytometry. Colony-forming 
      units were obtained to assess the bactericidal activity of propolis-treated DCs. 
      KEY FINDINGS: Propolis activated DCs in the presence of LPS, inducing NF-kB, 
      TNF-alpha, IL-6 and IL-10 production. The inhibition of hsa-miR-148a and hsa-miR-148b 
      abolished the inhibitory effects on HLA-DR and pro-inflammatory cytokines. The 
      increased expression of hsa-miR-155 may be correlated to the increase in TLR-4 
      and CD86 expression, maintaining LPS-induced expression of HLA-DR and CD40. Such 
      parameters may be involved in the increased bactericidal activity of DCs against 
      Streptococcus mutans. CONCLUSION: Propolis modulated the maturation and function 
      of DCs and may be useful in the initial steps of the immune response, providing a 
      novel approach to the development of DC-based strategies and for the discovery of 
      new immunomodulators.
CI  - (c) 2016 Royal Pharmaceutical Society.
FAU - Conti, Bruno J
AU  - Conti BJ
AD  - Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State 
      University (UNESP), Sao Paulo, Brazil.
FAU - Santiago, Karina B
AU  - Santiago KB
AD  - Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State 
      University (UNESP), Sao Paulo, Brazil.
FAU - Cardoso, Eliza O
AU  - Cardoso EO
AD  - Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State 
      University (UNESP), Sao Paulo, Brazil.
FAU - Freire, Paula P
AU  - Freire PP
AD  - Department of Morphology, Biosciences Institute, Sao Paulo State University 
      (UNESP), Sao Paulo, Brazil.
FAU - Carvalho, Robson F
AU  - Carvalho RF
AD  - Department of Morphology, Biosciences Institute, Sao Paulo State University 
      (UNESP), Sao Paulo, Brazil.
FAU - Golim, Marjorie A
AU  - Golim MA
AD  - Botucatu Blood Center, School of Medicine, Sao Paulo State University (UNESP), 
      Sao Paulo, Brazil.
FAU - Sforcin, Jose M
AU  - Sforcin JM
AD  - Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State 
      University (UNESP), Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20161007
PL  - England
TA  - J Pharm Pharmacol
JT  - The Journal of pharmacy and pharmacology
JID - 0376363
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (IL10 protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN148 microRNA, human)
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 9009-62-5 (Propolis)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - B7-2 Antigen/metabolism
MH  - CD40 Antigens/metabolism
MH  - Cells, Cultured
MH  - Cytokines/immunology/*metabolism
MH  - Dendritic Cells/*drug effects/immunology/metabolism/microbiology
MH  - Dose-Response Relationship, Drug
MH  - HLA-DR Antigens/metabolism
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Interleukin-6/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - NF-kappa B/immunology/*metabolism
MH  - Propolis/*pharmacology
MH  - Signal Transduction/drug effects
MH  - Streptococcus mutans/immunology/*pathogenicity
MH  - Toll-Like Receptor 4/immunology/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - dendritic cells
OT  - immunomodulation
OT  - propolis
EDAT- 2016/10/08 06:00
MHDA- 2017/05/17 06:00
CRDT- 2016/10/08 06:00
PHST- 2016/04/05 00:00 [received]
PHST- 2016/07/26 00:00 [accepted]
PHST- 2016/10/08 06:00 [pubmed]
PHST- 2017/05/17 06:00 [medline]
PHST- 2016/10/08 06:00 [entrez]
AID - 10.1111/jphp.12628 [doi]
PST - ppublish
SO  - J Pharm Pharmacol. 2016 Dec;68(12):1604-1612. doi: 10.1111/jphp.12628. Epub 2016 
      Oct 7.