PMID- 27717530
OWN - NLM
STAT- MEDLINE
DCOM- 20170220
LR  - 20220321
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 220
IP  - Pt A
DP  - 2017 Jan
TI  - Effect of silver nano-particles on soil microbial growth, activity and community 
      diversity in a sandy loam soil.
PG  - 504-513
LID - S0269-7491(16)31558-5 [pii]
LID - 10.1016/j.envpol.2016.09.094 [doi]
AB  - Silver nano-particles (AgNPs) are widely used in a range of consumer products as 
      a result of their antimicrobial properties. Given the broad spectrum of uses, 
      AgNPs have the potential for being released to the environment. As a result, 
      environmental risks associated with AgNPs need to be assessed to aid in the 
      development of regulatory guidelines. Research was performed to assess the 
      effects of AgNPs on soil microbial activity and diversity in a sandy loam soil 
      with an emphasis on using a battery of microbial tests involving multiple 
      endpoints. The test soil was spiked with PVP coated (0.3%) AgNPs at the following 
      concentrations of 49, 124, 287, 723 and 1815 mg Ag kg(-1) dry soil. Test controls 
      included an un-amended soil; soil amended with PVP equivalent to the highest PVP 
      concentration of the coated AgNP; and soil amended with humic acid, as 1.8% humic 
      acid was used as a suspension agent for the AgNPs. The impact on soil microbial 
      community was assessed using an array of tests including heterotrophic plate 
      counting, microbial respiration, organic matter decomposition, soil enzyme 
      activity, biological nitrification, community level physiological profiling 
      (CLPP), Ion Torrent DNA sequencing and denaturing gradient gel electrophoresis 
      (DGGE). An impact on microbial growth, activity and community diversity was 
      evident from 49 to 1815 mg kg(-1) with the median inhibitory concentrations 
      (IC50) as low as 20-31 mg kg(-1) depending on the test. AgNP showed a notable 
      impact on microbial functional and genomic diversity. Emergence of a silver 
      tolerant bacterium was observed at AgNP concentrations of 49-287 mg kg(-1) after 
      14-28 days of incubation, but not detectable at 723 and 1815 mg kg(-1). The 
      bacterium was identified as Rhodanobacter sp. The study highlighted the 
      effectiveness of using multiple microbial endpoints for inclusion to the 
      environmental risk assessment of nanomaterials.
CI  - Crown Copyright A(c) 2016. Published by Elsevier Ltd. All rights reserved.
FAU - Samarajeewa, A D
AU  - Samarajeewa AD
AD  - Biological Assessment and Standardization Section, Environment and Climate Change 
      Canada, 335, River Road, Ottawa, K1V 1C7, Ontario, Canada. Electronic address: 
      ajith.diassamarajeewa@canada.ca.
FAU - Velicogna, J R
AU  - Velicogna JR
AD  - Biological Assessment and Standardization Section, Environment and Climate Change 
      Canada, 335, River Road, Ottawa, K1V 1C7, Ontario, Canada.
FAU - Princz, J I
AU  - Princz JI
AD  - Biological Assessment and Standardization Section, Environment and Climate Change 
      Canada, 335, River Road, Ottawa, K1V 1C7, Ontario, Canada.
FAU - Subasinghe, R M
AU  - Subasinghe RM
AD  - Biological Assessment and Standardization Section, Environment and Climate Change 
      Canada, 335, River Road, Ottawa, K1V 1C7, Ontario, Canada.
FAU - Scroggins, R P
AU  - Scroggins RP
AD  - Biological Assessment and Standardization Section, Environment and Climate Change 
      Canada, 335, River Road, Ottawa, K1V 1C7, Ontario, Canada.
FAU - Beaudette, L A
AU  - Beaudette LA
AD  - Biological Assessment and Standardization Section, Environment and Climate Change 
      Canada, 335, River Road, Ottawa, K1V 1C7, Ontario, Canada.
LA  - eng
PT  - Journal Article
DEP - 20161004
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Bacteria/*drug effects/growth & development
MH  - *Biodiversity
MH  - Metal Nanoparticles/*adverse effects
MH  - Risk Assessment
MH  - Silver/*pharmacology
MH  - Soil/*chemistry
MH  - *Soil Microbiology
MH  - Soil Pollutants/*pharmacology
OTO - NOTNLM
OT  - Rhodanobacter sp.
OT  - Silver nano-particles (AgNPs)
OT  - Soil bacterial community
OT  - Toxicity
EDAT- 2016/10/09 06:00
MHDA- 2017/08/18 06:00
CRDT- 2016/10/09 06:00
PHST- 2016/07/05 00:00 [received]
PHST- 2016/09/23 00:00 [revised]
PHST- 2016/09/29 00:00 [accepted]
PHST- 2016/10/09 06:00 [pubmed]
PHST- 2017/08/18 06:00 [medline]
PHST- 2016/10/09 06:00 [entrez]
AID - S0269-7491(16)31558-5 [pii]
AID - 10.1016/j.envpol.2016.09.094 [doi]
PST - ppublish
SO  - Environ Pollut. 2017 Jan;220(Pt A):504-513. doi: 10.1016/j.envpol.2016.09.094. 
      Epub 2016 Oct 4.