PMID- 27717728
OWN - NLM
STAT- MEDLINE
DCOM- 20170404
LR  - 20181202
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 791
DP  - 2016 Nov 15
TI  - Bergenin attenuates renal injury by reversing mitochondrial dysfunction in 
      ethylene glycol induced hyperoxaluric rat model.
PG  - 611-621
LID - S0014-2999(16)30643-4 [pii]
LID - 10.1016/j.ejphar.2016.10.002 [doi]
AB  - Bergenin, isolated from Bergenia ligulata is a potent antioxidant and 
      antilithiatic agent. Present work was designed to establish the biochemical role 
      of bergenin on mitochondrial dysfunction in the ethylene glycol induced 
      hyperoxaluric rat model. Bergenin was administrated at a dose of 10mg/kg body wt 
      i.p. from 14th day of establishing the 28 days hyperoxaluria rat model. 
      alpha-Tocopherol was given as positive control at a dose of 100mg/kg body wt i.p. 
      Mitochondrial dysfunction was studied by evaluating the activities of respiratory 
      chain complexes, mitochondrial membrane potential and reactive oxygen species. 
      Histopathological analysis of the kidney tissue was done after Pizzolato 
      staining. Also, expression of monocyte chemoattractant protein -1(MCP-1) and 
      kidney injury marker protein (KIM-1) were studied and the levels of IL-1beta were 
      evaluated in kidney tissue homogenate. Mitochondrial dysfunction during stone 
      crystallization was evident by decreased activities of electron transport chain 
      complexes I, II and IV and augmented mitochondrial oxidative stress in 
      hyperoxaluric rats. Bergenin treatment significantly (P<0.05) restored the 
      activities of these complexes. Moreover, it curtailed the lipid peroxidation and 
      up regulated antioxidant levels, ameliorating the state of mitochondrial 
      dysfunction. The protective role of bergenin was also reinforced by reducing 
      IL-1beta production and expression of KIM-1 and MCP-1 in the renal tissue. The 
      findings of the present study provide evidence that bergenin exerted protective 
      effects in hyperoxaluria through mitochondrial protection that involves 
      attenuation of oxidative stress. Hence, it presented itself as an effective 
      remedy in combating urolithiasis.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Aggarwal, Deepika
AU  - Aggarwal D
AD  - Department of Biochemistry, Panjab University Chandigarh, 160014, India.
FAU - Gautam, Diksha
AU  - Gautam D
AD  - Department of Biochemistry, Panjab University Chandigarh, 160014, India.
FAU - Sharma, Minu
AU  - Sharma M
AD  - Department of Biochemistry, Panjab University Chandigarh, 160014, India.
FAU - Singla, S K
AU  - Singla SK
AD  - Department of Biochemistry, Panjab University Chandigarh, 160014, India. 
      Electronic address: singlask.biochem@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20161005
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Antioxidants)
RN  - 0 (Benzopyrans)
RN  - 0 (Biomarkers)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Reactive Oxygen Species)
RN  - AYI8EX34EU (Creatinine)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - FC72KVT52F (Ethylene Glycol)
RN  - L84RBE4IDC (bergenin)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology/therapeutic use
MH  - Benzopyrans/*pharmacology/therapeutic use
MH  - Biomarkers/metabolism
MH  - Chemokine CCL2/genetics/metabolism
MH  - Creatinine/metabolism
MH  - Cytoprotection/drug effects
MH  - Disease Models, Animal
MH  - Ethylene Glycol/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Hyperoxaluria/*chemically induced/drug therapy/metabolism/*pathology
MH  - Interleukin-1beta/metabolism
MH  - Kidney/*drug effects/*injuries/metabolism/pathology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Male
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mitochondria/*drug effects/pathology
MH  - Oxidation-Reduction/drug effects
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Bergenin
OT  - Hyperoxaluria
OT  - Mitochondrial dysfunction
OT  - Oxidative stress
OT  - Renal injury
EDAT- 2016/10/30 06:00
MHDA- 2017/04/05 06:00
CRDT- 2016/10/09 06:00
PHST- 2016/08/29 00:00 [received]
PHST- 2016/09/24 00:00 [revised]
PHST- 2016/10/03 00:00 [accepted]
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2017/04/05 06:00 [medline]
PHST- 2016/10/09 06:00 [entrez]
AID - S0014-2999(16)30643-4 [pii]
AID - 10.1016/j.ejphar.2016.10.002 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2016 Nov 15;791:611-621. doi: 10.1016/j.ejphar.2016.10.002. Epub 
      2016 Oct 5.