PMID- 27718047 OWN - NLM STAT- MEDLINE DCOM- 20170321 LR - 20181113 IS - 1572-994X (Electronic) IS - 0920-8569 (Linking) VI - 53 IP - 1 DP - 2017 Feb TI - Generation and evaluation of a genetically attenuated Newcastle disease virus rGM-VIIm as a genotype-matched vaccine. PG - 35-43 LID - 10.1007/s11262-016-1397-8 [doi] AB - Despite intensive vaccination campaigns, outbreaks of Newcastle disease (ND) have been frequently reported in China, especially of genotype VII that first emerged in the late 1990s. Given the dire need for vaccines against the circulating genotype VII virus, we developed an alternative method to recover a highly virulent recombinant GM (rGM) virus that involves a T7 system with a hammerhead ribozyme sequence introduced downstream of the T7 promoter. By changing the F(0) polybasic cleavage site RRQKR downward arrowF to the monobasic GRQGR downward arrowL, we generated a mutant virus (rGM-VIIm) that was found to be highly attenuated in chickens. The rGM-VIIm virus not only produced fourfold higher hemagglutination assay (HA) titers than the parental virus, but also exhibited genetic stability after 15 continuous passages in specific-pathogen-free (SPF) embryonated eggs. Whether live or inactivated, rGM-VIIm and LaSota vaccines can protect vaccinated birds from GM challenge infection. However, live and inactivated rGM-VIIm vaccines reduced virus shedding of the homologous challenge virus significantly better than the LaSota virus vaccine did. Altogether, our results suggest that rGM-VIIm vaccines could aid in the control of ND in China. FAU - Sun, Minhua AU - Sun M AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. AD - Guangdong Open Laboratory of Veterinary Public Health, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Baishigang Road, Guangzhou, Guangdong, China. FAU - Xiang, Bin AU - Xiang B AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Li, Yaling AU - Li Y AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Xie, Peng AU - Xie P AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Gao, Shimin AU - Gao S AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. AD - College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Tailuo Road, Taigu, Shanxi, China. FAU - Kang, Yinfeng AU - Kang Y AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. AD - Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, China. FAU - Gao, Pei AU - Gao P AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Li, Yanling AU - Li Y AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Wang, Zhaoxiong AU - Wang Z AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. AD - College of Animal Science, Yangtze University, 88 Jing Mi Road, Jingzhou, Hubei, China. FAU - Liang, Jianpeng AU - Liang J AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Yu, Deshui AU - Yu D AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Ren, Tao AU - Ren T AD - College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou, Guangdong, China. rentao6868@126.com. AD - National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. rentao6868@126.com. AD - Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. rentao6868@126.com. AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. rentao6868@126.com. LA - eng PT - Journal Article DEP - 20161007 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 RN - 0 (Antibodies, Viral) RN - 0 (DNA, Complementary) RN - 0 (Vaccines, Attenuated) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Antibodies, Viral/blood/immunology MH - Chickens MH - DNA, Complementary MH - Gene Order MH - Genome, Viral MH - *Genotype MH - Immunization MH - Mutation MH - Newcastle Disease/immunology/*prevention & control/virology MH - Newcastle disease virus/*genetics/*immunology MH - *Vaccines, Attenuated MH - Viral Load MH - Viral Vaccines/*genetics/*immunology MH - Virulence MH - Virus Replication MH - Virus Shedding OTO - NOTNLM OT - Genotype VII OT - Newcastle disease virus OT - Reverse genetics OT - Vaccine EDAT- 2016/10/09 06:00 MHDA- 2017/03/23 06:00 CRDT- 2016/10/09 06:00 PHST- 2016/04/18 00:00 [received] PHST- 2016/09/28 00:00 [accepted] PHST- 2016/10/09 06:00 [pubmed] PHST- 2017/03/23 06:00 [medline] PHST- 2016/10/09 06:00 [entrez] AID - 10.1007/s11262-016-1397-8 [pii] AID - 10.1007/s11262-016-1397-8 [doi] PST - ppublish SO - Virus Genes. 2017 Feb;53(1):35-43. doi: 10.1007/s11262-016-1397-8. Epub 2016 Oct 7.