PMID- 27720927
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20181202
IS  - 1549-9642 (Electronic)
IS  - 1549-9634 (Linking)
VI  - 13
IP  - 2
DP  - 2017 Feb
TI  - Facile and phase-defined determination of HLA alleles with 
      morpholino-functionalized nanoparticle probes.
PG  - 611-618
LID - S1549-9634(16)30161-7 [pii]
LID - 10.1016/j.nano.2016.09.009 [doi]
AB  - A number of human leukocyte antigen (HLA) gene alleles have been found to be 
      genetic risk markers for immunologically mediated drug hypersensitivity. Clinical 
      adoption of HLA pharmacogenomics requires facile and accurate allele screening 
      assays. As HLA genes are highly polymorphic, currently available methods are 
      usually labor-intensive and liable to generate false positives. Herein we report 
      a general strategy for screening HLA alleles with nanoparticle probes. Specific 
      HLA alleles can be identified by gauging three to five sequence variants. 
      Single-polymerase chain reaction (PCR) and dual-PCR methods have been proposed to 
      achieve phase-defined determination of the sequence variants. 
      Morpholino-functionalized gold nanoparticle probes allow for colorimetric and 
      highly specific detection. Assays for HLA-B*58:01 and HLA-B*15:02 have been 
      developed and validated with 49 selected human genomic DNA samples. The facile 
      nanoparticle probe-based assays can be implemented easily in molecular diagnostic 
      laboratories for accurate and cost-effective screening of HLA alleles.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Zu, Yanbing
AU  - Zu Y
AD  - Institute of Bioengineering and Nanotechnology, Singapore, Singapore.
FAU - Tan, Min-Han
AU  - Tan MH
AD  - Institute of Bioengineering and Nanotechnology, Singapore, Singapore.
FAU - Ren, Ee Chee
AU  - Ren EC
AD  - Singapore Immunology Network, 8A Biomedical Grove, Immunos, Singapore, Singapore.
FAU - Ying, Jackie Y
AU  - Ying JY
AD  - Institute of Bioengineering and Nanotechnology, Singapore, Singapore. Electronic 
      address: jyying@ibn.a-star.edu.sg.
LA  - eng
PT  - Journal Article
DEP - 20161005
PL  - United States
TA  - Nanomedicine
JT  - Nanomedicine : nanotechnology, biology, and medicine
JID - 101233142
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Morpholinos)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - HLA Antigens
MH  - HLA-B Antigens/*genetics
MH  - Humans
MH  - *Morpholinos
MH  - *Nanoparticles
MH  - Pharmacogenomic Testing/*methods
MH  - Polymerase Chain Reaction
OTO - NOTNLM
OT  - Colorimetric detection
OT  - HLA alleles
OT  - Morpholino-functionalized gold nanoparticles
OT  - Pharmacogenomics
OT  - Screening
EDAT- 2016/10/11 06:00
MHDA- 2017/10/03 06:00
CRDT- 2016/10/11 06:00
PHST- 2016/02/16 00:00 [received]
PHST- 2016/08/31 00:00 [revised]
PHST- 2016/09/18 00:00 [accepted]
PHST- 2016/10/11 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
PHST- 2016/10/11 06:00 [entrez]
AID - S1549-9634(16)30161-7 [pii]
AID - 10.1016/j.nano.2016.09.009 [doi]
PST - ppublish
SO  - Nanomedicine. 2017 Feb;13(2):611-618. doi: 10.1016/j.nano.2016.09.009. Epub 2016 
      Oct 5.