PMID- 27725685
OWN - NLM
STAT- MEDLINE
DCOM- 20180619
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Oct 11
TI  - Generation of transgenic marmosets expressing genetically encoded calcium 
      indicators.
PG  - 34931
LID - 10.1038/srep34931 [doi]
LID - 34931
AB  - Chronic monitoring of neuronal activity in the living brain with optical imaging 
      techniques became feasible owing to the continued development of genetically 
      encoded calcium indicators (GECIs). Here we report for the first time the 
      successful generation of transgenic marmosets (Callithrix jacchus), an important 
      nonhuman primate model in neurophysiological research, which were engineered to 
      express the green fluorescent protein (GFP)-based family of GECIs, GCaMP, under 
      control of either the CMV or the hSyn promoter. High titer lentiviral vectors 
      were produced, and injected into embryos collected from donor females. The 
      infected embryos were then transferred to recipient females. Eight transgenic 
      animals were born and shown to have stable and functional GCaMP expression in 
      several different tissues. Germline transmission of the transgene was confirmed 
      in embryos generated from two of the founder transgenic marmosets that reached 
      sexual maturity. These embryos were implanted into six recipient females, three 
      of which became pregnant and are in advanced stages of gestation. We believe 
      these transgenic marmosets will be invaluable non-human primate models in 
      neuroscience, allowing chronic in vivo monitoring of neural activity with 
      functional confocal and multi-photon optical microscopy imaging of intracellular 
      calcium dynamics.
FAU - Park, Jung Eun
AU  - Park JE
AD  - Cerebral Microcirculation Section, Laboratory of Functional and Molecular 
      Imaging, National Institute of Neurological Disorders and Stroke, National 
      Institutes of Health, Bethesda, MD, USA.
FAU - Zhang, Xian Feng
AU  - Zhang XF
AD  - Cerebral Microcirculation Section, Laboratory of Functional and Molecular 
      Imaging, National Institute of Neurological Disorders and Stroke, National 
      Institutes of Health, Bethesda, MD, USA.
FAU - Choi, Sang-Ho
AU  - Choi SH
AD  - Cerebral Microcirculation Section, Laboratory of Functional and Molecular 
      Imaging, National Institute of Neurological Disorders and Stroke, National 
      Institutes of Health, Bethesda, MD, USA.
FAU - Okahara, Junko
AU  - Okahara J
AD  - Department of Applied Developmental Biology, Central Institute for Experimental 
      Animals, Tonomachi, Kawasaki, Kanagawa 210-0821, Japan.
FAU - Sasaki, Erika
AU  - Sasaki E
AD  - Department of Applied Developmental Biology, Central Institute for Experimental 
      Animals, Tonomachi, Kawasaki, Kanagawa 210-0821, Japan.
AD  - Keio advanced Research Center, Keio University, Shinanomachi, Shinjuku-ku, Tokyo 
      160-8582, Japan.
FAU - Silva, Afonso C
AU  - Silva AC
AD  - Cerebral Microcirculation Section, Laboratory of Functional and Molecular 
      Imaging, National Institute of Neurological Disorders and Stroke, National 
      Institutes of Health, Bethesda, MD, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20161011
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - *Animals, Genetically Modified
MH  - Calcium/*metabolism
MH  - Callithrix/*genetics
MH  - Cytomegalovirus/genetics
MH  - Gene Expression
MH  - *Genes, Reporter
MH  - Green Fluorescent Proteins/*analysis/*genetics
MH  - Models, Animal
MH  - Neurophysiological Monitoring/methods
MH  - Optical Imaging/methods
MH  - Promoter Regions, Genetic
PMC - PMC5057151
EDAT- 2016/10/12 06:00
MHDA- 2018/06/21 06:00
PMCR- 2016/10/11
CRDT- 2016/10/12 06:00
PHST- 2016/05/12 00:00 [received]
PHST- 2016/09/20 00:00 [accepted]
PHST- 2016/10/12 06:00 [entrez]
PHST- 2016/10/12 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2016/10/11 00:00 [pmc-release]
AID - srep34931 [pii]
AID - 10.1038/srep34931 [doi]
PST - epublish
SO  - Sci Rep. 2016 Oct 11;6:34931. doi: 10.1038/srep34931.