PMID- 27725869
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220321
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 8
IP  - 9
DP  - 2016
TI  - MiR-300 suppresses laryngeal squamous cell carcinoma proliferation and metastasis 
      by targeting ROS1.
PG  - 3903-3911
AB  - Laryngeal squamous cell carcinoma (LSCC) is a common aggressive head and neck 
      cancer with high mortality and incidence. MicroRNAs (miRNAs) are short, 
      non-coding and endogenous RNAs that posttranscriptionally inhibit gene 
      expression. In this study, we showed that miR-300 expression was downregulated in 
      LSCC tissues compared with adjacent no-tumor tissues. MiR-300 overexpression 
      inhibited Hep-2 cell proliferation, as well as the expression of ki-67 and PCNA. 
      Moreover, overexpression of miR-300 repressed the cell invasion in Hep-2 cells. 
      We identified c-ros oncogene 1 receptor tyrosine kinase (ROS1) as a direct target 
      gene of miR-300 in Hep-2 cell. Furthermore, ROS1 expression was upregulated in 
      LSCC tissues compared with adjacent no-tumor tissues. Interesting, there were an 
      inverse correlation between ROS1 and miR-300 expression in the LSCC tissues. 
      Overexpression of ROS1 increased the Hep-2 cells proliferation and invasion. 
      Overexpression of ROS1 abrogated miR-300 induced cell growth and invasion 
      inhibition. Therefore, our data suggested that miR-300 acted as a tumor 
      suppressive gene in LSCC.
FAU - Ge, Wensheng
AU  - Ge W
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital 
      Liaocheng 252000, Shandong, China.
FAU - Han, Chaodong
AU  - Han C
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital 
      Liaocheng 252000, Shandong, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital 
      Liaocheng 252000, Shandong, China.
FAU - Zhang, Yunping
AU  - Zhang Y
AD  - Department of Dermatology, Liaocheng People's Hospital and EENT Hospital 
      Liaocheng 252000, Shandong, China.
LA  - eng
PT  - Journal Article
DEP - 20160915
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5040687
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - ROS1
OT  - miR-300
OT  - miRNAs
OT  - microRNAs
EDAT- 2016/10/12 06:00
MHDA- 2016/10/12 06:01
PMCR- 2016/09/15
CRDT- 2016/10/12 06:00
PHST- 2015/11/30 00:00 [received]
PHST- 2016/01/18 00:00 [accepted]
PHST- 2016/10/12 06:00 [entrez]
PHST- 2016/10/12 06:00 [pubmed]
PHST- 2016/10/12 06:01 [medline]
PHST- 2016/09/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2016 Sep 15;8(9):3903-3911. eCollection 2016.