PMID- 27727132
OWN - NLM
STAT- MEDLINE
DCOM- 20171113
LR  - 20180308
IS  - 1873-281X (Electronic)
IS  - 1472-9792 (Linking)
VI  - 101S
DP  - 2016 Dec
TI  - Comparing TSPOT assay results between an Elispot reader and manual counts.
PG  - S92-S98
LID - S1472-9792(16)30397-3 [pii]
LID - 10.1016/j.tube.2016.09.013 [doi]
AB  - BACKGROUND: The interferon gamma release assay, TSPOT.TB (TSPOT) can be read by 
      several methodologies, including an Elispot reader or manually by technician. We 
      compared the results from these two counting methods. METHODS: Automated and 
      manual TSPOT results among 2481 United States health care workers were compared. 
      Cohen's kappa coefficient was used to determine the inter-rater agreement. 
      Univariate and multiple logistic regression were used to investigate selected 
      variable contributions. RESULTS: No prognostic factors were associated with 
      agreement of TSPOT results between counting methods. Agreement between TSPOT 
      results were 92.3%, 89.5%, 93.0%, and 93.1% at baseline, and at follow-up at 6, 
      12, and 18 months, respectively. The inter-rater agreement for all test results 
      was good (kappa = 0.71). There was a significant difference between individual 
      technicians kappa coefficients (p < 0.001), but no significant increase in 
      agreement over time for technicians (p = 0.394). CONCLUSION: Commercial Elispot 
      readers and manual counts have good agreement of TSPOT results in a low TB burden 
      setting. Levels of agreement differed between individual technicians and 
      automated reader from moderate to very good, indicating borderline results may be 
      misinterpreted due to inter-rater variability. With no latent tuberculosis 
      infection (LTBI) gold standard, it cannot be determined if one TSPOT reading 
      method is better than another.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Agarwal, Saroochi
AU  - Agarwal S
AD  - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA; 
      University of Texas School of Public Health, Center for Infectious Diseases, 1200 
      Pressler St, Houston, TX, 77030, USA. Electronic address: 
      vagarwal@houstonmethodist.org.
FAU - Nguyen, Duc T
AU  - Nguyen DT
AD  - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. 
      Electronic address: dtnguyen6@houstonmethodist.org.
FAU - Lew, Justin D
AU  - Lew JD
AD  - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. 
      Electronic address: jdlew@houstonmethodist.org.
FAU - Teeter, Larry D
AU  - Teeter LD
AD  - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. 
      Electronic address: larry.teeter@dshs.state.tx.us.
FAU - Yamal, Jose-Miguel
AU  - Yamal JM
AD  - University of Texas School of Public Health, Center for Infectious Diseases, 1200 
      Pressler St, Houston, TX, 77030, USA. Electronic address: 
      jose-miguel.yamal@uth.tmc.edu.
FAU - Restrepo, Blanca I
AU  - Restrepo BI
AD  - University of Texas School of Public Health, Center for Infectious Diseases, 1200 
      Pressler St, Houston, TX, 77030, USA. Electronic address: 
      blanca.i.restrepo@uth.tmc.edu.
FAU - Brown, Eric L
AU  - Brown EL
AD  - University of Texas School of Public Health, Center for Infectious Diseases, 1200 
      Pressler St, Houston, TX, 77030, USA. Electronic address: 
      eric.l.brown@uth.tmc.edu.
FAU - Dorman, Susan E
AU  - Dorman SE
AD  - Johns Hopkins Medicine, 733 North Broadway, Baltimore, MD, 21205, USA. Electronic 
      address: dsusan1@jhmi.edu.
FAU - Graviss, Edward A
AU  - Graviss EA
AD  - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. 
      Electronic address: eagraviss@houstonmethodist.org.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
DEP - 20160928
PL  - Scotland
TA  - Tuberculosis (Edinb)
JT  - Tuberculosis (Edinburgh, Scotland)
JID - 100971555
SB  - IM
MH  - Adult
MH  - Automation, Laboratory/*instrumentation
MH  - Cross-Sectional Studies
MH  - Enzyme-Linked Immunospot Assay/*instrumentation
MH  - Female
MH  - Health Personnel
MH  - Humans
MH  - Interferon-gamma Release Tests/*instrumentation
MH  - Latent Tuberculosis/blood/*diagnosis/immunology/microbiology
MH  - Male
MH  - Middle Aged
MH  - Observer Variation
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
MH  - United States
OTO - NOTNLM
OT  - Agreement
OT  - Automated reader
OT  - IGRA
OT  - TSPOT
OT  - Tuberculosis
EDAT- 2016/10/12 06:00
MHDA- 2017/11/14 06:00
CRDT- 2016/10/12 06:00
PHST- 2016/10/12 06:00 [pubmed]
PHST- 2017/11/14 06:00 [medline]
PHST- 2016/10/12 06:00 [entrez]
AID - S1472-9792(16)30397-3 [pii]
AID - 10.1016/j.tube.2016.09.013 [doi]
PST - ppublish
SO  - Tuberculosis (Edinb). 2016 Dec;101S:S92-S98. doi: 10.1016/j.tube.2016.09.013. 
      Epub 2016 Sep 28.