PMID- 27727133 OWN - NLM STAT- MEDLINE DCOM- 20171113 LR - 20180308 IS - 1873-281X (Electronic) IS - 1472-9792 (Linking) VI - 101S DP - 2016 Dec TI - Differential positive TSPOT assay responses to ESAT-6 and CFP-10 in health care workers. PG - S83-S91 LID - S1472-9792(16)30396-1 [pii] LID - 10.1016/j.tube.2016.09.012 [doi] AB - BACKGROUND: The TSPOT.TB (TSPOT) diagnostic test for latent tuberculosis infection is based on a cell-mediated response to the Mycobacteria tuberculosis antigens, ESAT-6 and/or CFP-10, producing an "interferon-gamma footprint". We investigated the within-sample and within-subject variability of positive TSPOT assays due to the individual assay antigens' reactivity. METHODS: Positive TSPOT assay frequencies due to ESAT-6 or CFP-10 among health care workers (HCWs) at 6-month intervals for 18 months were compared. Differences in result interpretation (positive or negative) for ESAT-6 and CFP10 and potential prognostic factors were investigated. RESULTS: There were 576 positive results in 8805 TSPOT assays representing 2418 participants. A significant difference was detected in positive TSPOT results due to a positive response to either ESAT-6, CFP-10 or both antigens at baseline through 12 M (p < 0.001), but not for the 18 M follow-up. Gender, ethnicity, occupation, previous positive tuberculin skin test (TST) and study site were significantly associated with specific antigen positivity. CONCLUSIONS: Among our HCW samples with positive TSPOT assays, CFP-10 induced a larger proportion of positive TSPOT results than ESAT-6. Potential causes for this finding include: BCG vaccinated subpopulations, certain jobs, history of positive TST, U.S. birth, and study site. A high proportion of single-positive specimens may reflect false-positives results. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Agarwal, Saroochi AU - Agarwal S AD - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA; University of Texas School of Public Health, Center for Infectious Diseases, 1200 Pressler St, Houston, TX, 77030, USA. Electronic address: vagarwal@houstonmethodist.org. FAU - Nguyen, Duc T AU - Nguyen DT AD - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. Electronic address: dtnguyen6@houstonmethodist.org. FAU - Lew, Justin D AU - Lew JD AD - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. Electronic address: jdlew@houstonmethodist.org. FAU - Teeter, Larry D AU - Teeter LD AD - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. Electronic address: larry.teeter@dshs.state.tx.us. FAU - Yamal, Jose-Miguel AU - Yamal JM AD - University of Texas School of Public Health, Center for Infectious Diseases, 1200 Pressler St, Houston, TX, 77030, USA. Electronic address: jose-miguel.yamal@uth.tmc.edu. FAU - Restrepo, Blanca I AU - Restrepo BI AD - University of Texas School of Public Health, Center for Infectious Diseases, 1200 Pressler St, Houston, TX, 77030, USA. Electronic address: blanca.i.restrepo@uth.tmc.edu. FAU - Brown, Eric L AU - Brown EL AD - University of Texas School of Public Health, Center for Infectious Diseases, 1200 Pressler St, Houston, TX, 77030, USA. Electronic address: eric.l.brown@uth.tmc.edu. FAU - Dorman, Susan E AU - Dorman SE AD - Johns Hopkins Medicine, 733 North Broadway, Baltimore, MD, 21205, USA. Electronic address: dsusan1@jhmi.edu. FAU - Graviss, Edward A AU - Graviss EA AD - Houston Methodist Hospital Institute, 6670 Bertner Ave, Houston, TX, 77030, USA. Electronic address: eagraviss@houstonmethodist.org. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20160928 PL - Scotland TA - Tuberculosis (Edinb) JT - Tuberculosis (Edinburgh, Scotland) JID - 100971555 RN - 0 (Antigens, Bacterial) RN - 0 (Bacterial Proteins) RN - 0 (CFP-10 protein, Mycobacterium tuberculosis) RN - 0 (ESAT-6 protein, Mycobacterium tuberculosis) RN - 0 (IFNG protein, human) RN - 82115-62-6 (Interferon-gamma) SB - IM MH - Adolescent MH - Adult MH - Aged MH - Antigens, Bacterial/*immunology MH - Bacterial Proteins/*immunology MH - Cross-Sectional Studies MH - *Enzyme-Linked Immunospot Assay MH - False Positive Reactions MH - Female MH - *Health Personnel MH - Humans MH - Interferon-gamma/*blood/immunology MH - *Interferon-gamma Release Tests MH - Latent Tuberculosis/blood/*diagnosis/immunology/microbiology MH - Male MH - Middle Aged MH - Mycobacterium tuberculosis/*immunology MH - Predictive Value of Tests MH - Reproducibility of Results MH - Time Factors MH - Tuberculin Test MH - United States MH - Young Adult OTO - NOTNLM OT - CFP-10 OT - ESAT-6 OT - IGRA OT - TSPOT OT - Tuberculosis EDAT- 2016/10/12 06:00 MHDA- 2017/11/14 06:00 CRDT- 2016/10/12 06:00 PHST- 2016/10/12 06:00 [pubmed] PHST- 2017/11/14 06:00 [medline] PHST- 2016/10/12 06:00 [entrez] AID - S1472-9792(16)30396-1 [pii] AID - 10.1016/j.tube.2016.09.012 [doi] PST - ppublish SO - Tuberculosis (Edinb). 2016 Dec;101S:S83-S91. doi: 10.1016/j.tube.2016.09.012. Epub 2016 Sep 28.