PMID- 27727287
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20190212
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 10
DP  - 2016
TI  - Salivary Periodontopathic Bacteria in Children and Adolescents with Down 
      Syndrome.
PG  - e0162988
LID - 10.1371/journal.pone.0162988 [doi]
LID - e0162988
AB  - OBJECTIVE: To assess and compare salivary periodontopathic bacteria between 
      groups of Down syndrome and non-Down syndrome children and adolescents. MATERIALS 
      AND METHODS: This study included a sample of 30 Down syndrome children and 
      adolescents (G-DS) and 30 age- and sex-matched non-Down syndrome subjects (G-ND). 
      Clinical examination determined the gingival bleeding index (GBI) and plaque 
      index. Unstimulated whole saliva samples were collected from all participants. 
      The fluorescence in situ hybridization (FISH) technique identified the presence 
      and density of eight periodontopathic bacteria in saliva. The statistical 
      analysis included chi-square and Mann-Whitney U tests. RESULTS: In the G-DS 
      group, bleeding on probing was more frequent (p = 0.037) and higher densities of 
      Campylobacter rectus (p = 0.013), Porphyromonas gingivalis (p = 0.025), Treponema 
      denticola (p = 0.026), Fusobacterium nucleatum (p = 0.013), Prevotella intermedia 
      (p = 0.001) and Prevotella nigrescens (p = 0.008) were observed. Besides, in the 
      G-DS, the densities of bacteria from the orange complex were significantly higher 
      in the age group 3-7 years for F. nucleatum (p = 0.029), P. intermedia (p = 
      0.001) and P. nigrescens (p = 0.006). C. rectus was higher in the age group 8-12 
      years (p = 0.045). CONCLUSION: The results showed that children and adolescents 
      with Down syndrome have higher susceptibility to periodontal disease and number 
      of periodontopathic bacteria.
FAU - Faria Carrada, Camila
AU  - Faria Carrada C
AUID- ORCID: 0000-0002-3173-2904
AD  - School of Dentistry, Federal University of Juiz de Fora, Juiz de Fora, Minas 
      Gerais, Brazil.
FAU - Almeida Ribeiro Scalioni, Flavia
AU  - Almeida Ribeiro Scalioni F
AD  - Department of Pediatric Dentistry, School of Dentistry, Federal University of 
      Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
FAU - Evangelista Cesar, Dioneia
AU  - Evangelista Cesar D
AD  - Department of Ecology and Molecular Biology of Microorganisms, Institute of 
      Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Minas 
      Gerais, Brazil.
FAU - Lopes Devito, Karina
AU  - Lopes Devito K
AD  - Department of Dental Clinic, School of Dentistry, Federal University of Juiz de 
      Fora, Juiz de Fora, Minas Gerais, Brazil.
FAU - Ribeiro, Luiz Claudio
AU  - Ribeiro LC
AD  - Department of Statistics, Institute of Exact Sciences, Federal University of Juiz 
      de Fora, Juiz de Fora, Minas Gerais, Brazil.
FAU - Almeida Ribeiro, Rosangela
AU  - Almeida Ribeiro R
AD  - Department of Pediatric Dentistry, School of Dentistry, Federal University of 
      Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20161011
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Campylobacter rectus/genetics/isolation & purification
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - DNA, Bacterial/metabolism
MH  - Dental Plaque Index
MH  - Down Syndrome/*pathology
MH  - Female
MH  - Fusobacterium nucleatum/genetics/isolation & purification
MH  - Gram-Negative Bacteria/genetics/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Periodontal Diseases/*microbiology
MH  - Periodontal Index
MH  - Porphyromonas gingivalis/genetics/isolation & purification
MH  - Prevotella intermedia/genetics/isolation & purification
MH  - Saliva/*microbiology
MH  - Treponema denticola/genetics/isolation & purification
PMC - PMC5058504
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/10/12 06:00
MHDA- 2017/05/10 06:00
PMCR- 2016/10/11
CRDT- 2016/10/12 06:00
PHST- 2016/03/24 00:00 [received]
PHST- 2016/08/31 00:00 [accepted]
PHST- 2016/10/12 06:00 [entrez]
PHST- 2016/10/12 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2016/10/11 00:00 [pmc-release]
AID - PONE-D-16-10033 [pii]
AID - 10.1371/journal.pone.0162988 [doi]
PST - epublish
SO  - PLoS One. 2016 Oct 11;11(10):e0162988. doi: 10.1371/journal.pone.0162988. 
      eCollection 2016.