PMID- 27729070 OWN - NLM STAT- MEDLINE DCOM- 20170220 LR - 20220331 IS - 2047-783X (Electronic) IS - 0949-2321 (Print) IS - 0949-2321 (Linking) VI - 21 IP - 1 DP - 2016 Oct 12 TI - Cytokine and human leukocyte antigen (HLA) profile for graft-versus-host disease (GVHD) after organ transplantation. PG - 38 LID - 10.1186/s40001-016-0232-y [doi] LID - 38 AB - BACKGROUND: Graft-versus-host disease (GVHD) after liver and kidney transplantation has high mortality and causes diagnostic challenges. This study aims to describe the cytokine and human leukocyte antigen (HLA) profile in the GVHD after liver and kidney transplantation. METHODS: A high-throughput detection kit was applied and altogether 18 different cytokines were tested simultaneously. GVHD patients included 23 post-liver transplantation patients; 22 post-renal transplantation patients; The control patients include 22 hepatocellular carcinoma (HCC) patients without transplantation and 20 healthy controls. Their HLA characters were compared. RESULTS: The full spectrum of cytokines was present. The inflammatory markers were activated significantly in liver transplantation. The level of inflammatory markers in liver transplantation was higher than that in renal transplantation, HCC or healthy controls. GVHD was associated with the HLA characters; HLA characters are involved in liver GVHD occurrence and act as risk factors. CONCLUSION: Our findings confirmed that the inflammatory cytokines play a pathogenic role in GVHD and can be used as early diagnostic markers. The HLA mismatch acts as a risk factor in liver transplantation to predict GVHD occurrence. FAU - Chen, Xinhua AU - Chen X AD - Key Laboratory of Combined Multi-organ Transplantation, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Public Health, Hangzhou, China. AD - The Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. FAU - Meng, Xueqin AU - Meng X AD - Key Laboratory of Combined Multi-organ Transplantation, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Public Health, Hangzhou, China. AD - The Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. FAU - Xu, Yuning AU - Xu Y AD - Key Laboratory of Combined Multi-organ Transplantation, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Public Health, Hangzhou, China. AD - The Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. FAU - Xie, Haiyang AU - Xie H AD - Key Laboratory of Combined Multi-organ Transplantation, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Public Health, Hangzhou, China. AD - The Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. FAU - Yin, Shengyong AU - Yin S AD - Key Laboratory of Combined Multi-organ Transplantation, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Public Health, Hangzhou, China. AD - The Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. FAU - Li, Hongchun AU - Li H AD - Key Laboratory of Hepatobiliary Disease in Shenzhen, Shenzhen, 518112, China. FAU - Wu, Liming AU - Wu L AD - Key Laboratory of Combined Multi-organ Transplantation, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Public Health, Hangzhou, China. wlm@zju.edu.cn. AD - The Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. wlm@zju.edu.cn. FAU - Zheng, Shusen AU - Zheng S AD - Key Laboratory of Combined Multi-organ Transplantation, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Public Health, Hangzhou, China. shusenzheng@zju.edu.cn. AD - The Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. shusenzheng@zju.edu.cn. LA - eng PT - Journal Article DEP - 20161012 PL - England TA - Eur J Med Res JT - European journal of medical research JID - 9517857 RN - 0 (Biomarkers) RN - 0 (Cytokines) RN - 0 (HLA Antigens) SB - IM EIN - Eur J Med Res. 2017 Oct 3;22(1):37. PMID: 28974249 MH - Adult MH - Aged MH - Biomarkers/blood MH - Carcinoma, Hepatocellular/blood/immunology MH - Case-Control Studies MH - Cytokines/*blood MH - Female MH - Graft vs Host Disease/blood/*immunology/pathology MH - HLA Antigens/*blood MH - Humans MH - Kidney Transplantation/*adverse effects MH - Liver Neoplasms/blood/immunology MH - Liver Transplantation/*adverse effects MH - Male MH - Middle Aged MH - Skin/pathology PMC - PMC5059997 OTO - NOTNLM OT - Cytokine OT - Graft-versus-host disease (GVHD) OT - High-throughput OT - Human leukocyte antigen (HLA) OT - Multiplex immunoassay OT - Transplantation EDAT- 2016/10/13 06:00 MHDA- 2017/10/05 06:00 PMCR- 2016/10/12 CRDT- 2016/10/13 06:00 PHST- 2016/03/01 00:00 [received] PHST- 2016/09/30 00:00 [accepted] PHST- 2016/10/13 06:00 [entrez] PHST- 2016/10/13 06:00 [pubmed] PHST- 2017/10/05 06:00 [medline] PHST- 2016/10/12 00:00 [pmc-release] AID - 10.1186/s40001-016-0232-y [pii] AID - 232 [pii] AID - 10.1186/s40001-016-0232-y [doi] PST - epublish SO - Eur J Med Res. 2016 Oct 12;21(1):38. doi: 10.1186/s40001-016-0232-y.