PMID- 27729721
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 0971-5894 (Print)
IS  - 0974-0430 (Electronic)
IS  - 0974-0430 (Linking)
VI  - 22
IP  - 3
DP  - 2016 Jul
TI  - Phylogenetic analysis and photoregulation of siroheme biosynthesis genes: 
      uroporphyrinogen III methyltransferase and sirohydrochlorin ferrochelatase of 
      Arabidopsis thaliana.
PG  - 351-359
LID - 10.1007/s12298-016-0363-1 [doi]
AB  - Uroporphyrinogen III methyl transferase (UPM1) and Sirohydrochlorin 
      ferrochelatase (SIRB) are the important genes involved in the biosynthesis of 
      siroheme, the prosthetic group of nitrite reductases (NiR) and sulfite reductases 
      (SiR) involved in nitrogen and sulfur assimilation. Both UPM1 and SIRB could be 
      potential candidate genes targeted for sustainable agriculture especially in 
      N-deficient soil. The phylogenetic analysis revealed that these genes are highly 
      conserved among algae, bryophytes and vascular plants including dicots and 
      monocots. The Arabidopsis proteins UPM1 and SIRB have close similarity with 
      Camelina sativa followed by Brassica napus, Brassica rapa, and Brassica oleracea 
      of the family brassicaceae. The tissue specific expression studies revealed that 
      both the gene are expressed in stem, flower and silique and have highest 
      expression in leaves where the protein content is quite high. The in silico 
      promoter analysis revealed the presence of several light-responsive elements like 
      GATA box, G box, I box, SORLIP2, SORLIP5, SORLREP3 and SORLREP4. Therefore, 
      expression of both the genes was minimal in etiolated seedlings and was 
      upregulated in light. Photo-regulation of transcript abundance of UPM1 and SIRB 
      involved in the biosynthesis of siroheme the cofactor involved in 6 electron 
      reduction of NO(2)(-) and SO(3)(2-) by NiR and SiR is crucial as the gene 
      expression of latter two enzymes along with other N and S assimilatory enzymes 
      are also modulated by light.
FAU - Garai, Sampurna
AU  - Garai S
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067 India.
FAU - Joshi, Naveen Chandra
AU  - Joshi NC
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067 India.
FAU - Tripathy, Baishnab C
AU  - Tripathy BC
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067 India.
LA  - eng
PT  - Journal Article
DEP - 20160705
PL  - India
TA  - Physiol Mol Biol Plants
JT  - Physiology and molecular biology of plants : an international journal of 
      functional plant biology
JID - 101304333
PMC - PMC5039152
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - Light responsive elements
OT  - Phylogenetic tree
OT  - Sirohydrochlorin ferrochelatase
OT  - Uroporphyrinogen III methyltransferase
COIS- The authors declare no conflict of interest.
EDAT- 2016/10/13 06:00
MHDA- 2016/10/13 06:01
PMCR- 2017/07/01
CRDT- 2016/10/13 06:00
PHST- 2016/05/13 00:00 [received]
PHST- 2016/06/11 00:00 [revised]
PHST- 2016/06/20 00:00 [accepted]
PHST- 2016/10/13 06:00 [entrez]
PHST- 2016/10/13 06:00 [pubmed]
PHST- 2016/10/13 06:01 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - 363 [pii]
AID - 10.1007/s12298-016-0363-1 [doi]
PST - ppublish
SO  - Physiol Mol Biol Plants. 2016 Jul;22(3):351-359. doi: 10.1007/s12298-016-0363-1. 
      Epub 2016 Jul 5.