PMID- 27733800
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231111
IS  - 0972-8988 (Print)
IS  - 2231-0916 (Electronic)
IS  - 0972-8988 (Linking)
VI  - 9
IP  - 9
DP  - 2016 Sep
TI  - Differential response of immune-related genes to peptidoglycan and lipoteichoic 
      acid challenge in vitro.
PG  - 983-988
AB  - AIM: To study the effect of Staphylococcus aureus cell wall antigens, 
      peptidoglycan (PGN) and lipoteichoic acid (LTA) challenge on immune cells present 
      in bovine peripheral blood mononuclear cells (PBMCs). MATERIALS AND METHODS: In 
      this study, efforts have been made to investigate the effects of three 
      combinations (10+10, 20+20 and 30+30 mug/ml) of PGN and LTA obtained from S. 
      aureus. These antigens were used to challenge the bovine PBMCs. After 6 h of 
      incubation quantitative, real time-polymerase chain reaction was used to study 
      toll-like receptor 2 (TLR-2) and major cytokine mRNA expression in bovine PBMC 
      challenged with three different antigen blends. RESULTS: The results indicated 
      that mRNA level of interferon gamma is influenced by the expression of TLR-2 
      gene. Tumor necrosis factor-alpha (TNF-alpha), interleukin 10 (IL-10), and IL-8 genes 
      showed a maximum response at a dose of 10 mug of PGN and 10 mug of LTA challenge 
      per ml of culture medium. The outcome also suggests that both IL-10 and IL-8 
      followed the expression pattern of TNF-alpha. CONCLUSION: A dose of 10 mug of PGN and 
      10 mug of LTA per ml of culture medium was found to be most suitable for 
      challenging PBMC.
FAU - Sulabh, Sourabh
AU  - Sulabh S
AD  - Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, 
      Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
FAU - Bhushan, Bharat
AU  - Bhushan B
AD  - Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, 
      Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
FAU - Panigrahi, Manjit
AU  - Panigrahi M
AD  - Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, 
      Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
FAU - Verma, Ankita
AU  - Verma A
AD  - Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, 
      Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
FAU - Baba, Naseer Ahmad
AU  - Baba NA
AD  - Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, 
      Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
FAU - Kumar, Pushpendra
AU  - Kumar P
AD  - Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, 
      Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
LA  - eng
PT  - Journal Article
DEP - 20160917
PL  - India
TA  - Vet World
JT  - Veterinary world
JID - 101504872
PMC - PMC5057038
OTO - NOTNLM
OT  - expression
OT  - lipoteichoic acid
OT  - mastitis
OT  - peptidoglycan
OT  - peripheral blood mononuclear cell
EDAT- 2016/10/14 06:00
MHDA- 2016/10/14 06:01
PMCR- 2016/09/01
CRDT- 2016/10/14 06:00
PHST- 2016/05/05 00:00 [received]
PHST- 2016/08/01 00:00 [accepted]
PHST- 2016/10/14 06:00 [entrez]
PHST- 2016/10/14 06:00 [pubmed]
PHST- 2016/10/14 06:01 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - 10.14202/vetworld.2016.983-988 [doi]
PST - ppublish
SO  - Vet World. 2016 Sep;9(9):983-988. doi: 10.14202/vetworld.2016.983-988. Epub 2016 
      Sep 17.