PMID- 27737730
OWN - NLM
STAT- MEDLINE
DCOM- 20180723
LR  - 20180723
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 29
IP  - 9
DP  - 2017 Sep
TI  - Spatiotemporal expression profile of proteases and immunological, angiogenic, 
      hormonal and apoptotic mediators in rat placenta before and during intrauterine 
      trophoblast migration.
PG  - 1774-1786
LID - 10.1071/RD16280 [doi]
AB  - The gene and/or protein expression of proteases and immunological, angiogenic, 
      hormonal and apoptotic mediators was evaluated in rat placenta before and during 
      intrauterine trophoblast migration. The depth of interstitial and endovascular 
      intrauterine trophoblast invasion and the immunohistochemical expression of 
      vascular endothelial growth factor (VEGF), fetal liver kinase 1 (Flk1), 
      interferon (IFN)-gamma, migration inhibitory factor (MIF), and inducible nitric oxide 
      synthase (iNOS; also known as nitric oxide synthase (NOS) 2) were evaluated. In 
      addition, the expression of the Vegf, Flk1, placental growth factor (Pigf), 
      soluble fms-like tyrosine kinase 1 (sFlt1), placental lactogen 1 (Pl1), 
      proliferin-related protein (rPlf), placental leptin (Lep), Toll-like receptor 2 
      (Tlr2), Toll-like receptor 4 (Tlr4), Infg, Mif, tumour necrosis factor-alpha (Tnf), 
      interleukin-10 (Il10), Nos2, caspase 3 (Casp3), Bax, Bcl2, matrix 
      metalloproteinase 2 (Mmp2) and matrix metalloproteinase 9 (Mmp9) genes was 
      determined by real-time reverse transcription-polymerase chain reaction. At 10 
      days gestation, gene expression of Tlr2, Tlr4, Tnf, Infg, Il10, Casp3, Pigf, 
      sFlt1 and Lep (P<0.05) were higher than at 14 and/or 19 days of gestation. The 
      beginning of intrauterine trophoblast invasion, i.e., at 14 days of gestation, 
      coincided with higher gene and/or protein expression of MMP9, VEGF, Flk1, NOS2, 
      MIF, BAX and rPlf compared to days 10 and 19 (P<0.05). In contrast, gene 
      expression of Mmp2 and Pl1 was higher at the end of trophoblast invasion compared 
      to 10 and 14 days of gestation (P<0.05). In conclusion, before intrauterine 
      trophoblast migration, expression of TLRs and immunological and pro-apoptotic 
      mediators is higher, whereas the beginning of trophoblast migration is 
      characterised by higher expression of the pro-angiogenic factors NOS2 and MMP9. 
      In contrast, MMP2 and PL1 expression is higher at the end of intrauterine 
      trophoblast migration.
FAU - Silva, Juneo F
AU  - Silva JF
AD  - Laboratorio de Histologia Animal, Departamento de Ciencias Biologicas, 
      Universidade Estadual de Santa Cruz, Campus Soane Nazare de Andrade, Rodovia 
      Jorge Amado, Km 16, 45662-900, Ilheus, Bahia, Brazil.
FAU - Ocarino, Natalia M
AU  - Ocarino NM
AD  - Laboratorio de Patologia, Departamento de Clinica e Cirurgia Veterinarias, Escola 
      de Veterinaria, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida 
      Antonio Carlos, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
FAU - Serakides, Rogeria
AU  - Serakides R
AD  - Laboratorio de Patologia, Departamento de Clinica e Cirurgia Veterinarias, Escola 
      de Veterinaria, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida 
      Antonio Carlos, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Cytokines)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Cell Movement/*physiology
MH  - Cytokines/*metabolism
MH  - Female
MH  - Interferon-gamma/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Peptide Hydrolases/*metabolism
MH  - Placenta/cytology/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Trophoblasts/cytology/*metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
EDAT- 2016/10/16 06:00
MHDA- 2018/07/24 06:00
CRDT- 2016/10/15 06:00
PHST- 2016/07/14 00:00 [received]
PHST- 2016/09/19 00:00 [accepted]
PHST- 2016/10/16 06:00 [pubmed]
PHST- 2018/07/24 06:00 [medline]
PHST- 2016/10/15 06:00 [entrez]
AID - RD16280 [pii]
AID - 10.1071/RD16280 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2017 Sep;29(9):1774-1786. doi: 10.1071/RD16280.