PMID- 27738658
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2476-4108 (Print)
IS  - 2476-3772 (Electronic)
IS  - 2476-3772 (Linking)
VI  - 14
IP  - 9
DP  - 2016 Sep
TI  - Xeno-free culture condition for human bone marrow and umbilical cord 
      matrix-derived mesenchymal stem/stromal cells using human umbilical cord blood 
      serum.
PG  - 567-576
AB  - BACKGROUND: Fetal bovine serum (FBS) is widely used in cell culture laboratories, 
      risk of zoonotic infections and allergic side effects create obstacles for its 
      use in clinical trials. Therefore, an alternative supplement with proper inherent 
      growth-promoting activities is demanded. OBJECTIVE: To find FBS substitute, we 
      tested human umbilical cord blood serum (hUCS) for proliferation of human 
      umbilical cord matrix derived mesenchymal stem cells (hUC-MSCs) and human bone 
      marrow-derived mesenchymal cells (hBM-MSCs). MATERIALS AND METHODS: Umbilical 
      cord blood of healthy neonates, delivered by Caesarian section, was collected and 
      the serum was separated. hUC-MSCs and hBM-MSCs were isolated and characterized by 
      assessment of cell surface antigens by flow cytometry, alkaline phosphatase 
      activity and osteogenic/adipogenic differentiation potential. The cells were then 
      cultured in Iscove's Modified Dulbecco's Medium (IMDM) by conventional methods in 
      three preparations: 1- with hUCS, 2- with FBS, and 3- without serum supplements. 
      Cell proliferation was measured using WST-1 assay, and cell viability was 
      assessed by trypan blue staining. RESULTS: The cells cultured in hUCS and FBS 
      exhibited similar morphology and mesenchymal stem cells properties. WST-1 
      proliferation assay data showed no significant difference between the 
      proliferation rate of either cells following hUCS and FBS supplementation. Trypan 
      blue exclusion dye test also revealed no significant difference for viability 
      between hUCS and FBS groups. A significant difference was detected between the 
      proliferation rate of stem cells cultured in serum-supplemented medium compared 
      with serum-free medium. CONCLUSION: Our results indicate that human umbilical 
      cord serum can effectively support proliferation of hBM-MSCS and hUC-MSCs in 
      vitro and can be used as an appropriate substitute for FBS, especially in 
      clinical studies.
FAU - Esmaeli, Azadeh
AU  - Esmaeli A
AD  - Physiology Research Center, Institute of neuropharmacology, Kerman University of 
      Medical Sciences, Kerman, Iran. ; Kerman Student Research Center, Kerman 
      University of Medical Sciences, Kerman, Iran.
FAU - Moshrefi, Mojgan
AU  - Moshrefi M
AD  - Physiology Research Center, Institute of neuropharmacology, Kerman University of 
      Medical Sciences, Kerman, Iran. ; Research and Clinical Center of Infertility, 
      Shahid Sadoughi University of Medical Sciences, Yazd, Iran.; Neuroscience 
      Research Center, Institute of Neuropharmacology, Kerman University of Medical 
      Sciences, Kerman, Iran.
FAU - Shamsara, Ali
AU  - Shamsara A
AD  - Neuroscience Research Center, Institute of Neuropharmacology, Kerman University 
      of Medical Sciences, Kerman, Iran. ; Department of Anatomy, Afzalipour School of 
      Medicine, Kerman University of Medical Sciences, Kerman, Iran.
FAU - Eftekhar-Vaghefi, Seyed Hasan
AU  - Eftekhar-Vaghefi SH
AD  - Department of Anatomy, Afzalipour School of Medicine, Kerman University of 
      Medical Sciences, Kerman, Iran.
FAU - Nematollahi-Mahani, Seyed Noureddin
AU  - Nematollahi-Mahani SN
AD  - Physiology Research Center, Institute of neuropharmacology, Kerman University of 
      Medical Sciences, Kerman, Iran. ; Neuroscience Research Center, Institute of 
      Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. ; 
      Department of Anatomy, Afzalipour School of Medicine, Kerman University of 
      Medical Sciences, Kerman, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Int J Reprod Biomed
JT  - International journal of reproductive biomedicine
JID - 101679102
PMC - PMC5054293
OTO - NOTNLM
OT  - Bone marrow
OT  - Cell proliferation
OT  - Cord blood
OT  - Mesenchymal stem/stromal cells
OT  - Umbilical cord
OT  - Wharton jelly
COIS- The authors have no conflicting financial interest.
EDAT- 2016/10/16 06:00
MHDA- 2016/10/16 06:01
PMCR- 2016/09/01
CRDT- 2016/10/15 06:00
PHST- 2016/10/15 06:00 [entrez]
PHST- 2016/10/16 06:00 [pubmed]
PHST- 2016/10/16 06:01 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - ijrb-14-567 [pii]
PST - ppublish
SO  - Int J Reprod Biomed. 2016 Sep;14(9):567-576.