PMID- 27739494
OWN - NLM
STAT- MEDLINE
DCOM- 20180411
LR  - 20211103
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Oct 14
TI  - A novel IRS-1-associated protein, DGKzeta regulates GLUT4 translocation in 3T3-L1 
      adipocytes.
PG  - 35438
LID - 10.1038/srep35438 [doi]
LID - 35438
AB  - Insulin receptor substrates (IRSs) are major targets of insulin receptor tyrosine 
      kinases. Here we identified diacylglycerol kinase zeta (DGKzeta) as an 
      IRS-1-associated protein, and examined roles of DGKzeta in glucose transporter 4 
      (GLUT4) translocation to the plasma membrane. When DGKzeta was knocked-down in 
      3T3-L1 adipocytes, insulin-induced GLUT4 translocation was inhibited without 
      affecting other mediators of insulin-dependent signaling. Similarly, knockdown of 
      phosphatidylinositol 4-phosphate 5-kinase 1alpha (PIP5K1alpha), which had been reported 
      to interact with DGKzeta, also inhibited insulin-induced GLUT4 translocation. 
      Moreover, DGKzeta interacted with IRS-1 without insulin stimulation, but insulin 
      stimulation decreased this interaction. Over-expression of sDGKzeta (short-form 
      DGKzeta), which competed out DGKzeta from IRS-1, enhanced GLUT4 translocation without 
      insulin stimulation. Taking these results together with the data showing that 
      cellular PIP5K activity was correlated with GLUT4 translocation ability, we 
      concluded that IRS-1-associated DGKzeta prevents GLUT4 translocation in the absence 
      of insulin and that the DGKzeta dissociated from IRS-1 by insulin stimulation 
      enhances GLUT4 translocation through PIP5K1alpha activity.
FAU - Liu, TingYu
AU  - Liu T
AD  - Departments of Animal Sciences and Applied Biological Chemistry, Graduate School 
      of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Yu, BuChin
AU  - Yu B
AD  - Departments of Animal Sciences and Applied Biological Chemistry, Graduate School 
      of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Kakino, Mamoru
AU  - Kakino M
AD  - Departments of Animal Sciences and Applied Biological Chemistry, Graduate School 
      of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Fujimoto, Hitoshi
AU  - Fujimoto H
AD  - Departments of Animal Sciences and Applied Biological Chemistry, Graduate School 
      of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Ando, Yasutoshi
AU  - Ando Y
AD  - Departments of Animal Sciences and Applied Biological Chemistry, Graduate School 
      of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Hakuno, Fumihiko
AU  - Hakuno F
AD  - Departments of Animal Sciences and Applied Biological Chemistry, Graduate School 
      of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Takahashi, Shin-Ichiro
AU  - Takahashi SI
AD  - Departments of Animal Sciences and Applied Biological Chemistry, Graduate School 
      of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, 
      Bunkyo-ku, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161014
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, mouse)
RN  - 0 (Slc2a4 protein, mouse)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.107 (Diacylglycerol Kinase)
RN  - EC 2.7.1.107 (diacylglycerol kinase zeta, mouse)
RN  - EC 2.7.1.68 (1-phosphatidylinositol-4-phosphate 5-kinase)
SB  - IM
MH  - 3T3 Cells
MH  - Adipocytes/drug effects/*metabolism
MH  - Animals
MH  - CHO Cells
MH  - Cell Membrane/metabolism
MH  - Cricetinae
MH  - Cricetulus
MH  - Diacylglycerol Kinase/genetics/*metabolism
MH  - Glucose Transporter Type 4/*metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Insulin/pharmacology
MH  - Insulin Receptor Substrate Proteins/metabolism
MH  - Mice
MH  - Phosphotransferases (Alcohol Group Acceptor)/metabolism
MH  - Protein Transport
PMC - PMC5064357
EDAT- 2016/10/16 06:00
MHDA- 2018/04/12 06:00
PMCR- 2016/10/14
CRDT- 2016/10/15 06:00
PHST- 2016/07/27 00:00 [received]
PHST- 2016/09/29 00:00 [accepted]
PHST- 2016/10/15 06:00 [entrez]
PHST- 2016/10/16 06:00 [pubmed]
PHST- 2018/04/12 06:00 [medline]
PHST- 2016/10/14 00:00 [pmc-release]
AID - srep35438 [pii]
AID - 10.1038/srep35438 [doi]
PST - epublish
SO  - Sci Rep. 2016 Oct 14;6:35438. doi: 10.1038/srep35438.