PMID- 27741312
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 10
DP  - 2016
TI  - Aqueous Extract of Gumiganghwal-tang, a Traditional Herbal Medicine, Reduces 
      Pulmonary Fibrosis by Transforming Growth Factor-beta1/Smad Signaling Pathway in 
      Murine Model of Chronic Asthma.
PG  - e0164833
LID - 10.1371/journal.pone.0164833 [doi]
LID - e0164833
AB  - Gumiganghwal-tang is a traditional herbal prescription that is used widely for 
      the treatment of the common cold and inflammatory diseases in Korea and other 
      Asian countries. In this study, we investigated the protective effects of a 
      Gumiganghwal-tang aqueous extract (GGTA) against airway inflammation and 
      pulmonary fibrosis using a mouse model of chronic asthma. Chronic asthma was 
      modeled in BALB/c mice via sensitization/challenge with an intraperitoneal 
      injection of 1% ovalbumin (OVA) and inhalation of nebulized 1% OVA for 4 weeks. 
      GGTA (100 mg/kg or 200 mg/kg) was also administered by oral gavage once a day for 
      4 weeks. We investigated the number of inflammatory cells, production of T-helper 
      type 2 (Th2) cytokines, chemokine and the total transforming growth factor-beta1 
      (TGF-beta1) in bronchoalveolar lavage fluid (BALF); the levels of immunoglobulin E 
      (IgE) in the plasma; the infiltration of inflammatory cells in lung tissue; and 
      the expression of TGF-beta1, Smad-3, and collagen in lung tissue. Our results 
      revealed that GGTA lowered the recruitment of inflammatory cells (particularly, 
      lymphocyte); and decreased the production of Th2 cytokines, chemokine and total 
      TGF-beta1; and attenuated the levels of total and OVA-specific IgE; and decreased 
      the infiltration of inflammatory cells. Moreover, GGTA significantly reduced the 
      expression of TGF-beta1 and Smad-3, and lowered collagen deposition. These results 
      indicate that GGTA reduces airway inflammation and pulmonary fibrosis by 
      regulating Th2 cytokines production and the TGF-beta1/Smad-3 pathway, thus providing 
      a potential treatment for chronic asthma.
FAU - Jeon, Woo-Young
AU  - Jeon WY
AUID- ORCID: 0000-0002-3321-3788
AD  - K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic 
      of Korea.
FAU - Shin, In-Sik
AU  - Shin IS
AD  - College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of 
      Korea.
FAU - Shin, Hyeun-Kyoo
AU  - Shin HK
AD  - K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic 
      of Korea.
FAU - Jin, Seong Eun
AU  - Jin SE
AD  - K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic 
      of Korea.
FAU - Lee, Mee-Young
AU  - Lee MY
AD  - K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic 
      of Korea.
LA  - eng
PT  - Journal Article
DEP - 20161014
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Plant Extracts)
RN  - 0 (Smad Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (kumi-kyokatsu-to)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Asthma/drug therapy/immunology
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Chemokines/analysis
MH  - Chronic Disease
MH  - Collagen/metabolism
MH  - Cytokines/analysis
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology/therapeutic use
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Immunoglobulin E/blood
MH  - Lung/drug effects/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
MH  - Plant Extracts/chemistry/*pharmacology/therapeutic use
MH  - Pulmonary Fibrosis/prevention & control
MH  - Signal Transduction/*drug effects
MH  - Smad Proteins/*metabolism
MH  - Th2 Cells/cytology/immunology/metabolism
MH  - Transforming Growth Factor beta1/*metabolism
PMC - PMC5065144
COIS- The authors declare that there is no conflict of, or competing financial 
      interests regarding, the publication of this paper.
EDAT- 2016/10/16 06:00
MHDA- 2017/05/23 06:00
PMCR- 2016/10/14
CRDT- 2016/10/15 06:00
PHST- 2016/02/12 00:00 [received]
PHST- 2016/10/01 00:00 [accepted]
PHST- 2016/10/15 06:00 [entrez]
PHST- 2016/10/16 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
PHST- 2016/10/14 00:00 [pmc-release]
AID - PONE-D-16-06119 [pii]
AID - 10.1371/journal.pone.0164833 [doi]
PST - epublish
SO  - PLoS One. 2016 Oct 14;11(10):e0164833. doi: 10.1371/journal.pone.0164833. 
      eCollection 2016.