PMID- 27742409
OWN - NLM
STAT- MEDLINE
DCOM- 20170321
LR  - 20171116
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 193
DP  - 2016 Dec 4
TI  - Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of 
      the AMPK signaling pathway.
PG  - 691-699
LID - S0378-8741(16)31274-0 [pii]
LID - 10.1016/j.jep.2016.10.039 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: The seed of Entada phaseoloides (L.) Merr. 
      (Entada phaseoloides) has been long used as a folk medicine for the treatment of 
      Diabetes mellitus by Chinese ethnic minorities. Recent reports have demonstrated 
      that total saponins from Entada phaseoloides (TSEP) could reduce fasting blood 
      glucose in type 2 diabetic rats. However, the mechanism has not been fully 
      elucidated. The aim of this study was to explore the underlying mechanisms of 
      TSEP on type 2 Diabetes mellitus (T2DM). MATERIALS AND METHODS: Primary mouse 
      hepatocytes and HepG2 cells were used to investigate the effects of TSEP on 
      gluconeogenesis. After treatment with TSEP, glucose production, genes expression 
      levels of Glucose-6-phosphatase (G6pase) and Phosphoenoylpyruvate carboxykinase 
      (Pepck) were detected. The efficacy and underlying mechanism of TSEP on 
      AMP-activated protein kinase (AMPK) signaling pathway were determinated. RESULTS: 
      TSEP significantly inhibited glucose production and the gluconeogenic gene 
      expression. Treatment with TSEP elevated the phosphorylation of AMPK, which in 
      turn promoted the phosphorylation of acetyl coenzyme A (ACC) and Akt/glycogen 
      synthase kinase 3beta (GSK3beta), respectively. Furthermore, TSEP reduced lipid 
      accumulation and improved insulin sensitivity in hepatocytes. CONCLUSION: These 
      findings provide evidence that TSEP exerts an antidiabetic effect by suppressing 
      hepatic gluconeogenesis via the AMPK signaling pathway.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Zheng, Tao
AU  - Zheng T
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      Hubei, China; Department of Pharmacology, School of Basic Medicine, Tongji 
      Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 
      China.
FAU - Hao, Xincai
AU  - Hao X
AD  - College of Pharmacy, Hubei University of Medicine, Shiyan, Hubei, China.
FAU - Wang, Qibin
AU  - Wang Q
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      Hubei, China.
FAU - Chen, Li
AU  - Chen L
AD  - Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      Hubei, China.
FAU - Jin, Si
AU  - Jin S
AD  - Department of Pharmacology, School of Basic Medicine, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei, China; Department of 
      Endocrinology, Institute of Geriatric Medicine, Liyuan Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, Hubei, China. 
      Electronic address: jinsi@hust.edu.cn.
FAU - Bian, Fang
AU  - Bian F
AD  - Department of Pharmacology, School of Basic Medicine, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei, China; The 
      Affiliated Hospital of Xiangyang Central Hospital of Hubei College of Arts and 
      Sciences, Xiangyang, China. Electronic address: bianfang45@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20161011
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Hypoglycemic Agents)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/isolation & purification/*pharmacology/therapeutic use
MH  - Fabaceae/*chemistry
MH  - Gluconeogenesis/*drug effects
MH  - Hep G2 Cells
MH  - Hepatocytes/*drug effects/enzymology/metabolism
MH  - Humans
MH  - Hypoglycemic Agents/isolation & purification/*pharmacology/therapeutic use
MH  - Lipid Metabolism/drug effects
MH  - Mice
MH  - Primary Cell Culture
MH  - Seeds/chemistry
MH  - Signal Transduction
OTO - NOTNLM
OT  - AMP-activated protein kinase
OT  - Entada phaseoloides (L.) Merr.
OT  - Gluconeogenesis
OT  - Saponins
OT  - Type 2 Diabetes mellitus
EDAT- 2016/10/16 06:00
MHDA- 2017/03/23 06:00
CRDT- 2016/10/16 06:00
PHST- 2016/05/27 00:00 [received]
PHST- 2016/10/09 00:00 [revised]
PHST- 2016/10/10 00:00 [accepted]
PHST- 2016/10/16 06:00 [pubmed]
PHST- 2017/03/23 06:00 [medline]
PHST- 2016/10/16 06:00 [entrez]
AID - S0378-8741(16)31274-0 [pii]
AID - 10.1016/j.jep.2016.10.039 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2016 Dec 4;193:691-699. doi: 10.1016/j.jep.2016.10.039. Epub 
      2016 Oct 11.