PMID- 27746164
OWN - NLM
STAT- MEDLINE
DCOM- 20170718
LR  - 20191210
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Linking)
VI  - 439
DP  - 2016 Dec
TI  - Type of monocyte immunomagnetic separation affects the morphology of 
      monocyte-derived dendritic cells, as investigated by scanning electron 
      microscopy.
PG  - 79-82
LID - S0022-1759(16)30160-0 [pii]
LID - 10.1016/j.jim.2016.10.004 [doi]
AB  - Dendritic cells (DCs) are increasingly being used for multiple applications and 
      are useful tools for many immunotherapeutic strategies. The understanding of the 
      possible impact of the DCs-generation methods on the biological capacities of 
      these cells is therefore essential. Although the immunomagnetic separation is 
      regarded as a fast and accurate method yielding cells with the high purity and 
      efficiency, still little is known about its impact on the properties of the 
      generated DCs. The aim of this study was to compare the morphology of the 
      monocyte derived dendritic cells (MoDCs), generated from monocytes selected with 
      anti-CD14 mAbs (positive separation) and treated with anti-CD3, -CD7, -CD16, 
      -CD19, -CD56, -CD123, glycophorin A (negative separation), using laser scanning 
      microscopy. We found that the type of the immunomagnetic separation method used 
      strongly influences the shape and cell dimension of the MoDCs. We observed that 
      the height of both immature and LPS-matured DCs generated from monocytes isolated 
      by negative separation was significantly higher compared to the cells obtained by 
      positive separation.
CI  - Copyright A(c) 2016 Elsevier B.V. All rights reserved.
FAU - Kowalewicz-Kulbat, M
AU  - Kowalewicz-Kulbat M
AD  - Department of Immunology and Infectious Biology, University of Lodz, ul. Banacha 
      12/16, 90-237 Lodz, Poland. Electronic address: mkow@biol.uni.lodz.pl.
FAU - Ograczyk, E
AU  - Ograczyk E
AD  - Department of Immunology and Infectious Biology, University of Lodz, ul. Banacha 
      12/16, 90-237 Lodz, Poland. Electronic address: ela.ograczyk@gmail.com.
FAU - Krawczyk, K
AU  - Krawczyk K
AD  - Department of Immunology and Infectious Biology, University of Lodz, ul. Banacha 
      12/16, 90-237 Lodz, Poland. Electronic address: k_krawczyk@biol.uni.lodz.pl.
FAU - Rudnicka, W
AU  - Rudnicka W
AD  - Department of Immunology and Infectious Biology, University of Lodz, ul. Banacha 
      12/16, 90-237 Lodz, Poland. Electronic address: rudw@biol.uni.lodz.pl.
FAU - Fol, M
AU  - Fol M
AD  - Department of Immunology and Infectious Biology, University of Lodz, ul. Banacha 
      12/16, 90-237 Lodz, Poland. Electronic address: folmarc@biol.uni.lodz.pl.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20161013
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (GYPA protein, human)
RN  - 0 (Glycophorins)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, CD/immunology
MH  - *Cell Shape/drug effects
MH  - *Cell Size/drug effects
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/immunology/*ultrastructure
MH  - Glycophorins/immunology
MH  - Humans
MH  - Immunomagnetic Separation/*methods
MH  - Lipopolysaccharides/pharmacology
MH  - *Microscopy, Electron, Scanning
MH  - Monocytes/drug effects/immunology/*ultrastructure
OTO - NOTNLM
OT  - Dendritic cells
OT  - Magnetic-activated cell sorting
OT  - Scanning electron microscopy
EDAT- 2016/10/18 06:00
MHDA- 2017/07/19 06:00
CRDT- 2016/10/18 06:00
PHST- 2016/08/23 00:00 [received]
PHST- 2016/09/27 00:00 [revised]
PHST- 2016/10/11 00:00 [accepted]
PHST- 2016/10/18 06:00 [pubmed]
PHST- 2017/07/19 06:00 [medline]
PHST- 2016/10/18 06:00 [entrez]
AID - S0022-1759(16)30160-0 [pii]
AID - 10.1016/j.jim.2016.10.004 [doi]
PST - ppublish
SO  - J Immunol Methods. 2016 Dec;439:79-82. doi: 10.1016/j.jim.2016.10.004. Epub 2016 
      Oct 13.