PMID- 27748844
OWN - NLM
STAT- MEDLINE
DCOM- 20170317
LR  - 20170317
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 36
IP  - 6
DP  - 2016 Dec
TI  - Differential glycolytic profile and Warburg effect in papillary thyroid carcinoma 
      cell lines.
PG  - 3673-3681
LID - 10.3892/or.2016.5142 [doi]
AB  - Acceleration of glycolysis is a characteristic of neoplasia. Previous studies 
      have shown that a metabolic shift occurs in many tumors and correlates with a 
      negative prognosis. The present study aimed to investigate the glycolytic profile 
      of thyroid carcinoma cell lines. We investigated glycolytic and oxidative 
      parameters of two thyroid carcinoma papillary cell lines (BCPAP and TPC1) and the 
      non-tumor cell line NTHY-ori. All carcinoma cell lines showed higher rates of 
      glycolysis efficiency, when compared to NTHY-ori, as assessed by a higher rate of 
      glucose consumption and lactate production. The BCPAP cell line presented higher 
      rates of growth, as well as elevated intracellular ATP levels, compared to the 
      TPC1 and NTHY-ori cells. We found that glycolysis and activities of pentose 
      phosphate pathway (PPP) regulatory enzymes were significantly different among the 
      carcinoma cell lines, particularly in the mitochondrial hexokinase (HK) activity 
      which was higher in the BCPAP cells than that in the TPC1 cell line which showed 
      a balanced distribution of HK activity between cytoplasmic and mitochondrial 
      subcellular localizations. However, TPC1 had higher levels of glucose‑6-phosphate 
      dehydrogenase activity, suggesting that the PPP is elevated in this cell type. 
      Using high resolution respirometry, we observed that the Warburg effect was 
      present in the BCPAP and TPC1 cells, characterized by low oxygen consumption and 
      high reactive oxygen species production. Overall, these results indicate that 
      both thyroid papillary carcinoma cell lines showed a glycolytic profile. Of note, 
      BCPAP cells presented some relevant differences in cell metabolism compared to 
      TPC1 cells, mainly related to higher mitochondrial-associated HK activity.
FAU - Coelho, Raquel Guimaraes
AU  - Coelho RG
AD  - Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, 
      Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
FAU - Cazarin, Juliana de Menezes
AU  - Cazarin JM
AD  - Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, 
      Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
FAU - Cavalcanti de Albuquerque, Joao Paulo Albuquerque
AU  - Cavalcanti de Albuquerque JP
AD  - Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, 
      Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
FAU - de Andrade, Bruno Moulin
AU  - de Andrade BM
AD  - Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, 
      Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
FAU - Carvalho, Denise P
AU  - Carvalho DP
AD  - Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, 
      Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20161004
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Adenosine Triphosphate/biosynthesis
MH  - Carcinoma, Papillary/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - *Glycolysis
MH  - Hexokinase/metabolism
MH  - Humans
MH  - Mitochondria/metabolism
MH  - Oxidation-Reduction
MH  - Oxygen Consumption
MH  - Thyroid Neoplasms/*metabolism
EDAT- 2016/10/18 06:00
MHDA- 2017/03/18 06:00
CRDT- 2016/10/18 06:00
PHST- 2016/04/13 00:00 [received]
PHST- 2016/06/09 00:00 [accepted]
PHST- 2016/10/18 06:00 [pubmed]
PHST- 2017/03/18 06:00 [medline]
PHST- 2016/10/18 06:00 [entrez]
AID - 10.3892/or.2016.5142 [doi]
PST - ppublish
SO  - Oncol Rep. 2016 Dec;36(6):3673-3681. doi: 10.3892/or.2016.5142. Epub 2016 Oct 4.