PMID- 27748859
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20170417
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 5
DP  - 2016 Nov
TI  - Human parvovirus B19 antibodies induce altered membrane protein expression and 
      apoptosis of BeWo trophoblasts.
PG  - 4399-4406
LID - 10.3892/mmr.2016.5787 [doi]
AB  - Human parvovirus B19 (B19) is harmful during pregnancy since it can be vertically 
      transmitted to the developing fetus. In addition, the anti‑B19 antibodies induced 
      by B19 infection are believed to have a cytopathic role in B19 transmission; 
      however, knowledge regarding the effects of anti‑B19 antibodies during pregnancy 
      is limited. To investigate the possible roles of anti‑B19 antibodies during 
      pregnancy, the present study examined the effects of anti‑B19‑VP1 unique region 
      (VP1u), anti‑B19‑VP2 and anti‑B19‑nonstructural protein 1 (NS1) immunoglobulin G 
      (IgG) antibodies on BeWo trophoblasts. Briefly, BeWo trophoblasts were incubated 
      with purified IgG against B19‑VP1u, B19‑VP2 and B19‑NS1. Subsequently, the 
      expression of surface proteins and apoptotic molecules were assessed in BeWo 
      trophoblasts using flow cytometry, ELISA and western blotting. The expression 
      levels of human leukocyte antigen (HLA)‑G were significantly increased on BeWo 
      trophoblasts treated with rabbit anti‑B19‑VP1u IgG, and were unchanged in those 
      treated with rabbit anti‑B19‑NS1 and anti‑B19‑VP2 IgG, as compared with the 
      control group. Furthermore, the expression levels of globoside (P blood group 
      antigen) and cluster of differentiation (CD)29 (beta1 integrin) were significantly 
      increased in BeWo trophoblasts treated with rabbit anti‑B19‑NS1 and anti‑B19‑VP2 
      IgG, whereas only CD29 was also significantly increased in cells treated with 
      anti‑B19‑VP1u IgG. In addition, the number of cells at sub‑G1 phase; caspase‑3 
      activity; and the expression of intrinsic and extrinsic apoptotic molecules, 
      including Fas‑associated death domain protein, activated caspase‑8, activated 
      caspase‑3, B‑cell lymphoma 2‑associated X protein, cytochrome c, apoptotic 
      peptidase activating factor 1 and activated caspase‑9, were significantly 
      increased in BeWo trophoblasts treated with anti‑B19‑VP1u and anti‑B19‑NS1 IgG. 
      In conclusion, the present study demonstrated that antibodies against B19 may 
      have a crucial role in pathological processes during pregnancy. These findings 
      may help to elucidate the mechanisms underlying transmission of the B19 virus 
      during pregnancy.
FAU - Tzang, Bor-Show
AU  - Tzang BS
AD  - Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical 
      University, Taichung 40201, Taiwan, R.O.C.
FAU - Chiang, Szu-Yi
AU  - Chiang SY
AD  - Division of Neurology, Department of Internal Medicine, Chi Mei Medical Center, 
      Liouying 73663, Taiwan, R.O.C.
FAU - Chan, Hsu-Chin
AU  - Chan HC
AD  - Department of Biochemistry, School of Medicine, China Medical University, 
      Taichung 40402, Taiwan, R.O.C.
FAU - Liu, Chung-Hsien
AU  - Liu CH
AD  - Department of Obstetrics and Gynecology, Chung Shan Medical University and Chung 
      Shan Medical University Hospital, Taichung 40201, Taiwan, R.O.C.
FAU - Hsu, Tsai-Ching
AU  - Hsu TC
AD  - Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical 
      University, Taichung 40201, Taiwan, R.O.C.
LA  - eng
PT  - Journal Article
DEP - 20160926
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Capsid Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Membrane Proteins)
RN  - 0 (NS1 protein, parvovirus)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (capsid protein VP1, parvovirus B19)
RN  - 0 (capsid protein VP2, parvovirus B19)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/administration & dosage/immunology
MH  - Antibodies, Viral/*immunology/isolation & purification
MH  - Apoptosis/immunology
MH  - Capsid Proteins/antagonists & inhibitors/immunology
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Immunoglobulin G/administration & dosage
MH  - Membrane Proteins/biosynthesis/genetics/immunology
MH  - Parvoviridae Infections/*immunology/transmission/virology
MH  - Parvovirus B19, Human/*immunology/isolation & purification/pathogenicity
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/*immunology/therapy/virology
MH  - Rabbits
MH  - Trophoblasts/*immunology/virology
MH  - Viral Nonstructural Proteins/antagonists & inhibitors/immunology
EDAT- 2016/10/26 06:00
MHDA- 2017/04/18 06:00
CRDT- 2016/10/26 06:00
PHST- 2015/09/04 00:00 [received]
PHST- 2016/09/13 00:00 [accepted]
PHST- 2016/10/26 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
PHST- 2016/10/26 06:00 [entrez]
AID - 10.3892/mmr.2016.5787 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Nov;14(5):4399-4406. doi: 10.3892/mmr.2016.5787. Epub 2016 Sep 
      26.