PMID- 27752254
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 7
DP  - 2016
TI  - Norovirus-Specific Memory T Cell Responses in Adult Human Donors.
PG  - 1570
LID - 1570
AB  - Norovirus (NoV) is a leading cause of acute gastroenteritis in people of all ages 
      worldwide. NoV-specific serum antibodies which block the binding of NoV 
      virus-like particles (VLPs) to the cell receptors have been thoroughly 
      investigated. In contrast, only a few publications are available on the NoV 
      capsid VP1 protein-specific T cell responses in humans naturally infected with 
      the virus. Freshly isolated peripheral blood mononuclear cells of eight healthy 
      adult human donors previously exposed to NoV were stimulated with purified VLPs 
      derived from NoV GII.4-1999, GII.4-2012 (Sydney), and GI.3, and IFN-gamma production 
      was measured by an ELISPOT assay. In addition, 76 overlapping synthetic peptides 
      spanning the entire 539-amino acid sequence of GII.4 VP1 were pooled into 
      two-dimensional matrices and used to identify putative T cell epitopes. Seven of 
      the eight subjects produced IFN-gamma in response to the peptides and five subjects 
      produced IFN-gamma in response to the VLPs of the same origin. In general, stronger T 
      cell responses were induced with the peptides in each donor compared to the VLPs. 
      A CD8(+) T cell epitope in the shell domain of the VP1 
      ((134)SPSQVTMFPHIIVDVRQL(151)) was identified in two subjects, both having human 
      leukocyte antigen (HLA)-A( *)02:01 allele. To our knowledge, this is the first 
      report using synthetic peptides to study NoV-specific T cell responses in human 
      subjects and identify T cell epitopes.
FAU - Malm, Maria
AU  - Malm M
AD  - Vaccine Research Center, University of Tampere Tampere, Finland.
FAU - Tamminen, Kirsi
AU  - Tamminen K
AD  - Vaccine Research Center, University of Tampere Tampere, Finland.
FAU - Vesikari, Timo
AU  - Vesikari T
AD  - Vaccine Research Center, University of Tampere Tampere, Finland.
FAU - Blazevic, Vesna
AU  - Blazevic V
AD  - Vaccine Research Center, University of Tampere Tampere, Finland.
LA  - eng
PT  - Journal Article
DEP - 20161003
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5045929
OTO - NOTNLM
OT  - ELISPOT IFN-gamma
OT  - PBMC
OT  - T cell epitope
OT  - VLP
OT  - cellular immunity
OT  - norovirus
OT  - peptide pools
EDAT- 2016/10/19 06:00
MHDA- 2016/10/19 06:01
PMCR- 2016/10/03
CRDT- 2016/10/19 06:00
PHST- 2016/06/29 00:00 [received]
PHST- 2016/09/20 00:00 [accepted]
PHST- 2016/10/19 06:00 [pubmed]
PHST- 2016/10/19 06:01 [medline]
PHST- 2016/10/19 06:00 [entrez]
PHST- 2016/10/03 00:00 [pmc-release]
AID - 10.3389/fmicb.2016.01570 [doi]
PST - epublish
SO  - Front Microbiol. 2016 Oct 3;7:1570. doi: 10.3389/fmicb.2016.01570. eCollection 
      2016.