PMID- 27752902
OWN - NLM
STAT- MEDLINE
DCOM- 20170330
LR  - 20181113
IS  - 1993-1352 (Electronic)
IS  - 1672-0733 (Linking)
VI  - 36
IP  - 5
DP  - 2016 Oct
TI  - Small interfering RNA targeting TNF-alpha gene significantly attenuates renal 
      ischemia-reperfusion injury in mice.
PG  - 634-638
LID - 10.1007/s11596-016-1638-z [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been found to be centrally involved in 
      the development of ischemia-reperfusion injury (IRI)-induced inflammation and 
      apoptosis. Knockdown of TNF-alpha gene using small interfering RNA (siRNA) may 
      protect renal IRI. Renal IRI was induced in mice by clamping the left renal 
      pedicle for 25 or 35 min. TNF-alpha siRNA was administered intravenously to silence 
      the expression of TNF-alpha. The therapeutic effects of siRNA were evaluated in terms 
      of renal function, histological examination, and overall survival following 
      lethal IRI. A single systemic injection of TNF-alpha siRNA resulted in significant 
      knockdown of TNF-alpha expression in ischemia-reperfusion injured kidney. In 
      comparison with control mice, levels of BUN and serum creatinine were 
      significantly reduced in mice treated with siRNA. Pathological examination 
      demonstrated that tissue damage caused by IRI was markedly reduced as a result of 
      TNF-alpha siRNA treatment. Furthermore, survival experiments showed that nearly 90% 
      of control mice died from lethal IRI, whereas more than 50% of siRNApretreated 
      mice survived until the end of the eight-day observation period. We have 
      demonstrated for the first time that silencing TNF-alpha by specific siRNA can 
      significantly reduce renal IRI and protect mice against lethal kidney ischemia, 
      highlighting the potential for siRNA-based clinical therapy.
FAU - Hou, Ling
AU  - Hou L
AD  - Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430030, China.
FAU - Chen, Gang
AU  - Chen G
AD  - Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China. 
      gchen@tjh.tjmu.edu.cn.
AD  - Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, 430030, 
      China. gchen@tjh.tjmu.edu.cn.
AD  - Key Laboratory of Organ Transplantation, Ministry of Public Health, Wuhan, 
      430030, China. gchen@tjh.tjmu.edu.cn.
FAU - Feng, Biao
AU  - Feng B
AD  - Departments of Surgery, Microbiology and Immunology, and Pathology, University of 
      Western Ontario, London, Canada.
FAU - Zhang, Xu-Sheng
AU  - Zhang XS
AD  - Departments of Surgery, Microbiology and Immunology, and Pathology, University of 
      Western Ontario, London, Canada.
FAU - Zheng, Xiu-Fen
AU  - Zheng XF
AD  - Departments of Surgery, Microbiology and Immunology, and Pathology, University of 
      Western Ontario, London, Canada.
FAU - Xiang, Ying
AU  - Xiang Y
AD  - Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China.
AD  - Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, 430030, 
      China.
AD  - Key Laboratory of Organ Transplantation, Ministry of Public Health, Wuhan, 
      430030, China.
FAU - Zhao, Guang-Yuan
AU  - Zhao GY
AD  - Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China.
FAU - Min, Wei-Ping
AU  - Min WP
AD  - Departments of Surgery, Microbiology and Immunology, and Pathology, University of 
      Western Ontario, London, Canada.
LA  - eng
PT  - Journal Article
DEP - 20161018
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Disease Models, Animal
MH  - Genetic Therapy
MH  - Humans
MH  - Inflammation/*genetics/pathology/therapy
MH  - Kidney/drug effects/injuries/pathology
MH  - Mice
MH  - RNA, Small Interfering/*administration & dosage/genetics
MH  - Reperfusion Injury/*genetics/pathology/therapy
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/*genetics
OTO - NOTNLM
OT  - ischemia-reperfusion injury
OT  - kidney
OT  - small interfering RNA
OT  - tumor necrosis factor-alpha
EDAT- 2016/10/19 06:00
MHDA- 2017/03/31 06:00
CRDT- 2016/10/19 06:00
PHST- 2016/07/02 00:00 [received]
PHST- 2016/08/22 00:00 [revised]
PHST- 2016/10/19 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
PHST- 2016/10/19 06:00 [entrez]
AID - 10.1007/s11596-016-1638-z [pii]
AID - 10.1007/s11596-016-1638-z [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2016 Oct;36(5):634-638. doi: 
      10.1007/s11596-016-1638-z. Epub 2016 Oct 18.