PMID- 27754828
OWN - NLM
STAT- MEDLINE
DCOM- 20170215
LR  - 20181113
IS  - 1840-4812 (Electronic)
IS  - 1512-8601 (Print)
IS  - 1512-8601 (Linking)
VI  - 16
IP  - 4
DP  - 2016 Nov 10
TI  - Small interfering RNA-mediated silencing of nicotinamide 
      phosphoribosyltransferase (NAMPT) and lysosomal trafficking regulator (LYST) 
      induce growth inhibition and apoptosis in human multiple myeloma cells: A 
      preliminary study.
PG  - 268-275
LID - 10.17305/bjbms.2016.1568 [doi]
AB  - Multiple myeloma (MM) is a malignancy of B lymphocytes or plasma cells. Our 
      array-based comparative genomic hybridization findings revealed chromosomal gains 
      at 7q22.3 and 1q42.3, where nicotinamide (NAM) phosphoribosyltransferase (NAMPT) 
      and lysosomal trafficking regulator (LYST) genes are localized, respectively. 
      This led us to further study the functions of these genes in myeloma cells. NAMPT 
      is a key enzyme involved in nicotinamide adenine dinucleotide salvage pathway, 
      and it is frequently overexpressed in human cancers. In contrast, little is known 
      about the function of LYST in cancer. The expression of LYST is shown to affect 
      lysosomal size, granule size, and autophagy in human cells. In this study, the 
      effects of small interfering RNA (siRNA)-mediated silencing of NAMPT and LYST on 
      cell proliferation and apoptosis were evaluated in RPMI 8226 myeloma cells. 
      Transfection efficiencies were determined by quantitative real time reverse 
      transcriptase PCR. Cell proliferation was determined using MTT assay, while 
      apoptosis was analyzed with flow cytometry using Annexin V-fluorescein 
      isothiocyanate/propidium iodide assay. The NAMPT protein expression in 
      siRNA-treated cells was estimated by enzyme-linked immunosorbent assay. Our 
      results showed that NAMPT and LYST were successfully knockdown by siRNA 
      transfection (p < 0.05). NAMPT or LYST gene silencing significantly inhibited 
      cell proliferation and induced apoptosis in RPMI 8226 cells (p < 0.05). Silencing 
      of NAMPT gene also decreased NAMPT protein levels (p < 0.01). Our study 
      demonstrated that NAMPT and LYST play pivotal roles in the molecular pathogenesis 
      of MM. This is the first report describing the possible functions of LYST in 
      myelomagenesis and its potential role as a therapeutic target in MM.
FAU - Bong, Ivyna Pau Ni
AU  - Bong IP
AD  - Haematology Unit, Cancer Research Centre, Institute for Medical Research, Kuala 
      Lumpur, Malaysia; Department of Genetics and Molecular Biology, Institute of 
      Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 
      Malaysia. ivyna@imr.gov.my.
FAU - Ng, Ching Ching
AU  - Ng CC
FAU - Fakiruddin, Shaik Kamal
AU  - Fakiruddin SK
FAU - Lim, Moon Nian
AU  - Lim MN
FAU - Zakaria, Zubaidah
AU  - Zakaria Z
LA  - eng
PT  - Journal Article
DEP - 20161110
PL  - Bosnia and Herzegovina
TA  - Bosn J Basic Med Sci
JT  - Bosnian journal of basic medical sciences
JID - 101200947
RN  - 0 (Cytokines)
RN  - 0 (LYST protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Vesicular Transport Proteins)
RN  - EC 2.4.2.12 (Nicotinamide Phosphoribosyltransferase)
RN  - EC 2.4.2.12 (nicotinamide phosphoribosyltransferase, human)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cytokines/*genetics
MH  - Gene Knockdown Techniques
MH  - *Gene Silencing
MH  - Genetic Therapy
MH  - Humans
MH  - Multiple Myeloma/*genetics/*therapy
MH  - Neoplasm Proteins/biosynthesis
MH  - Nicotinamide Phosphoribosyltransferase/*genetics
MH  - RNA, Small Interfering/*pharmacology
MH  - Vesicular Transport Proteins/*genetics
PMC - PMC5136762
EDAT- 2016/10/19 06:00
MHDA- 2017/02/16 06:00
PMCR- 2016/11/01
CRDT- 2016/10/19 06:00
PHST- 2016/07/28 00:00 [received]
PHST- 2016/08/20 00:00 [accepted]
PHST- 2016/08/18 00:00 [revised]
PHST- 2016/10/19 06:00 [pubmed]
PHST- 2017/02/16 06:00 [medline]
PHST- 2016/10/19 06:00 [entrez]
PHST- 2016/11/01 00:00 [pmc-release]
AID - BJBMS-16-268 [pii]
AID - 10.17305/bjbms.2016.1568 [doi]
PST - epublish
SO  - Bosn J Basic Med Sci. 2016 Nov 10;16(4):268-275. doi: 10.17305/bjbms.2016.1568.