PMID- 27756710
OWN - NLM
STAT- MEDLINE
DCOM- 20170220
LR  - 20171215
IS  - 1469-0691 (Electronic)
IS  - 1198-743X (Linking)
VI  - 23
IP  - 2
DP  - 2017 Feb
TI  - Is Ureaplasma spp. the leading causative agent of acute chorioamnionitis in women 
      with preterm birth?
PG  - 119.e1-119.e7
LID - S1198-743X(16)30480-3 [pii]
LID - 10.1016/j.cmi.2016.10.010 [doi]
AB  - OBJECTIVES: Aim of this study was to detect microorganisms in fetal membranes and 
      placental tissue in preterm chorioamnionitis by combining fluorescence in situ 
      hybridization (FISH) with broad range PCR. The combination of the two molecular 
      techniques enables identification and localization of the microorganisms within 
      the tissue, confirming their clinical relevance. METHODS: In a prospective cohort 
      study, we compared 31 women with preterm premature rupture of membranes or 
      preterm labour and preterm delivery by caesarean section with a control group of 
      26 women undergoing elective caesarean section at term. Fetal membranes and 
      placental tissue were analysed by FISH and broad range 16S rRNA-gene PCR and 
      sequencing. RESULTS: For 20 women in the preterm group, caesarean section was 
      performed because of a clinical diagnosis of chorioamnionitis. Microorganisms 
      were detected in the tissues by both molecular techniques in 11 out of 20 women. 
      Among those, Ureaplasma spp. was most abundant, with five cases that remained 
      culture-negative and would have been missed by routine diagnostic procedures. 
      Other infections were caused by Staphylococcus aureus, Streptococcus mitis or 
      Escherichia coli. FISH and PCR were negative for all women without suspected 
      chorioamnionitis and for the control group. CONCLUSIONS: Combination of FISH with 
      broad-range PCR and sequencing permitted unambiguous identification of the 
      causative microorganisms in chorioamnionitis. The high prevalence of Ureaplasma 
      spp. should lead to a re-evaluation of its clinical significance and possible 
      therapeutic consequences.
CI  - Copyright (c) 2016 European Society of Clinical Microbiology and Infectious 
      Diseases. Published by Elsevier Ltd. All rights reserved.
FAU - Kikhney, J
AU  - Kikhney J
AD  - Biofilmzentrum, German Heart Institute Berlin, Berlin, Germany; Institut fur 
      Mikrobiologie und Hygiene, Charite - Universitatsmedizin Berlin, Campus Charite 
      Benjamin Franklin, Berlin, Germany.
FAU - von Schoning, D
AU  - von Schoning D
AD  - Klinik fur Geburtsmedizin, Charite - Universitatsmedizin Berlin, Campus Charite 
      Mitte, Berlin, Germany.
FAU - Steding, I
AU  - Steding I
AD  - Klinik fur Geburtsmedizin, Charite - Universitatsmedizin Berlin, Campus Charite 
      Mitte, Berlin, Germany.
FAU - Schulze, J
AU  - Schulze J
AD  - Biofilmzentrum, German Heart Institute Berlin, Berlin, Germany.
FAU - Petrich, A
AU  - Petrich A
AD  - Institut fur Mikrobiologie und Hygiene, Charite - Universitatsmedizin Berlin, 
      Campus Charite Benjamin Franklin, Berlin, Germany.
FAU - Hiergeist, A
AU  - Hiergeist A
AD  - Institute of Clinical Microbiology and Hygiene - University Hospital Regensburg, 
      Regensburg, Germany.
FAU - Reischl, U
AU  - Reischl U
AD  - Institute of Clinical Microbiology and Hygiene - University Hospital Regensburg, 
      Regensburg, Germany.
FAU - Moter, A
AU  - Moter A
AD  - Biofilmzentrum, German Heart Institute Berlin, Berlin, Germany. Electronic 
      address: moter@dhzb.de.
FAU - Thomas, A
AU  - Thomas A
AD  - Klinik fur Geburtsmedizin, Charite - Universitatsmedizin Berlin, Campus Charite 
      Mitte, Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20161015
PL  - England
TA  - Clin Microbiol Infect
JT  - Clinical microbiology and infection : the official publication of the European 
      Society of Clinical Microbiology and Infectious Diseases
JID - 9516420
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Chorioamnionitis/*diagnosis/*microbiology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Placenta/microbiology
MH  - Pregnancy
MH  - *Pregnancy Complications, Infectious
MH  - *Premature Birth
MH  - Prospective Studies
MH  - RNA, Ribosomal, 16S
MH  - Risk Factors
MH  - *Ureaplasma/classification/genetics
MH  - Ureaplasma Infections/*diagnosis/*microbiology
MH  - Young Adult
OTO - NOTNLM
OT  - 16S rRNA
OT  - Chorioamnionitis
OT  - Fluorescence in situ hybridization
OT  - Neonatal mortality
OT  - Preterm birth
OT  - Ureaplasma
EDAT- 2016/10/21 06:00
MHDA- 2017/12/16 06:00
CRDT- 2016/10/21 06:00
PHST- 2016/07/22 00:00 [received]
PHST- 2016/09/30 00:00 [revised]
PHST- 2016/10/01 00:00 [accepted]
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2017/12/16 06:00 [medline]
PHST- 2016/10/21 06:00 [entrez]
AID - S1198-743X(16)30480-3 [pii]
AID - 10.1016/j.cmi.2016.10.010 [doi]
PST - ppublish
SO  - Clin Microbiol Infect. 2017 Feb;23(2):119.e1-119.e7. doi: 
      10.1016/j.cmi.2016.10.010. Epub 2016 Oct 15.