PMID- 27757778 OWN - NLM STAT- MEDLINE DCOM- 20170414 LR - 20201209 IS - 1573-0832 (Electronic) IS - 0301-486X (Linking) VI - 182 IP - 3-4 DP - 2017 Apr TI - Paracoccidioides brasiliensis-associated dermatitis and lymphadenitis in a dog. PG - 425-434 LID - 10.1007/s11046-016-0075-7 [doi] AB - Paracoccidioidomycosis (PCM) is an endemic disease of humans from Latin America that is caused by Paracoccidioides brasiliensis and P. lutzii, with most cases of PCM in domestic animals being associated with P. brasiliensis. This study presents the clinical, cytological, mycological, serological, and molecular findings associated with P. brasiliensis in a dog from Southern Brazil. Fine needle biopsies were collected from the skin and several lymph nodes of a 5-year-old female Labrador dog that had enlargement of most superficial lymph nodes. Cytology of the skin and lymph nodes revealed pyogranulomatous dermatitis and lymphadenitis associated with fine-necked, budding fungal structures consistent with the Paracoccidioides genus of organisms; mycological culture derived from the lymph node aspirate demonstrated similar budding structures. Serological assays using exoantigens obtained from the fungal culture demonstrated that the fungal organisms derived from the lymph node were antigenically similar to P. brasiliensis by immunodiffusion and Western blot. A PCR assay, using the fungal culture as input, amplified a partial segment of the internal transcribed spacer 1 and 2 regions of P. brasiliensis; direct sequencing and phylogenetic analyses confirmed the PCR product as P. brasiliensis. The combined cytological, mycological, serological, and molecular findings confirmed a diagnosis of fungal dermatitis and lymphadenitis due to P. brasiliensis in this dog. This case represents the third description of clinical PCM in dogs and the first confirmation of mycotic dermatitis associated with P. brasiliensis in this species. The participation of dogs in the possible dissemination of PCM is reviewed, and it is proposed that dogs are probable accidental hosts in the epidemiological cycle associated with P. brasiliensis. FAU - Headley, Selwyn Arlington AU - Headley SA AD - Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 km 380, Campus Universitario, PO Box 10.011, Londrina, Parana, 86057-970, Brazil. selwyn.headley@uel.br. AD - Multi-User Animal Health Laboratory, Organic Tissue Processing Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Parana, Brazil. selwyn.headley@uel.br. FAU - Pretto-Giordano, Lucienne Garcia AU - Pretto-Giordano LG AD - Laboratory of Mycology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - Di Santis, Giovana Wingeter AU - Di Santis GW AD - Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 km 380, Campus Universitario, PO Box 10.011, Londrina, Parana, 86057-970, Brazil. AD - Multi-User Animal Health Laboratory, Organic Tissue Processing Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - Gomes, Lucas Alecio AU - Gomes LA AD - Small Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - Macagnan, Rafaela AU - Macagnan R AD - Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - da Nobrega, Daniela Farias AU - da Nobrega DF AD - Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 km 380, Campus Universitario, PO Box 10.011, Londrina, Parana, 86057-970, Brazil. FAU - Leite, Katherine Moura AU - Leite KM AD - Small Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - de Alcantara, Brigida Kussumoto AU - de Alcantara BK AD - Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - Itano, Eiko Nakagawa AU - Itano EN AD - Laboratory of Applied Immunology, Department of Pathological Sciences, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - Alfieri, Amauri Alcindo AU - Alfieri AA AD - Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Parana, Brazil. AD - Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Parana, Brazil. FAU - Ono, Mario Augusto AU - Ono MA AD - Laboratory of Applied Immunology, Department of Pathological Sciences, Universidade Estadual de Londrina, Londrina, Parana, Brazil. LA - eng PT - Case Reports PT - Journal Article DEP - 20161018 PL - Netherlands TA - Mycopathologia JT - Mycopathologia JID - 7505689 RN - 0 (Antigens, Fungal) RN - 0 (DNA, Fungal) RN - 0 (DNA, Ribosomal Spacer) SB - IM MH - Animals MH - Antigens, Fungal/analysis MH - Biopsy, Fine-Needle MH - Brazil MH - Cluster Analysis MH - DNA, Fungal/chemistry/genetics MH - DNA, Ribosomal Spacer/chemistry/genetics MH - Dermatitis/etiology/pathology/*veterinary MH - Dog Diseases/*diagnosis/microbiology/*pathology MH - Dogs MH - Female MH - Histocytochemistry MH - Immunoassay MH - Lymph Nodes/microbiology/pathology MH - Lymphadenitis/etiology/pathology/*veterinary MH - Microbiological Techniques MH - Microscopy MH - Paracoccidioides/*isolation & purification MH - Paracoccidioidomycosis/etiology/pathology/*veterinary MH - Phylogeny MH - Sequence Analysis, DNA MH - Skin/microbiology/pathology OTO - NOTNLM OT - Canine paracoccidioidomycosis OT - Cytological diagnosis OT - Epidemiology OT - Fungal dermatitis EDAT- 2016/10/21 06:00 MHDA- 2017/04/15 06:00 CRDT- 2016/10/21 06:00 PHST- 2016/06/20 00:00 [received] PHST- 2016/10/02 00:00 [accepted] PHST- 2016/10/21 06:00 [pubmed] PHST- 2017/04/15 06:00 [medline] PHST- 2016/10/21 06:00 [entrez] AID - 10.1007/s11046-016-0075-7 [pii] AID - 10.1007/s11046-016-0075-7 [doi] PST - ppublish SO - Mycopathologia. 2017 Apr;182(3-4):425-434. doi: 10.1007/s11046-016-0075-7. Epub 2016 Oct 18.