PMID- 27757997
OWN - NLM
STAT- MEDLINE
DCOM- 20170921
LR  - 20170921
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 52 Suppl 2
DP  - 2017 Apr
TI  - Determination of the normal reference interval for anti-Mullerian hormone (AMH) 
      in bitches and use of AMH as a potential predictor of litter size.
PG  - 35-40
LID - 10.1111/rda.12822 [doi]
AB  - Anti-Mullerian hormone (AMH) is a reliable endocrine marker of ovarian reserve in 
      many species with extensive literature in both humans and cattle. However, there 
      are no known hormonal predictors of ovarian reserve and potential reproductive 
      performance in the bitch. A prospective cohort study was performed involving 155 
      intact bitches of various ages (range 1.2-7.6 years) and breeds that were 
      presented for routine breeding management over a one-year period. All bitches 
      were artificially inseminated with frozen or fresh semen using the transcervical 
      insemination (TCI) technique. AMH concentrations were measured using a 
      commercially available canine AMH ELISA (Ansh Labs((R)) , Texas, USA), which we 
      validated prior to performing the study. The reference interval (RI) for AMH for 
      all bitches in the study, regardless of body weight, was 2.9-21.1 ng/ml. There 
      was a significant effect of bitch size and age on AMH concentrations. The RI for 
      giant breeds was significantly (p < .01) lower (1.75-15.6 ng/ml) than small-sized 
      (5.6-24.2 ng/ml), medium-sized (4.3-23.7 ng/ml) and large-sized (4.3-21.0 ng/ml) 
      bitches. The mean AMH concentration in bitches less than 4 years of age was 
      12.4 ng/ml, whereas the mean AMH concentration in bitches older than 4 years of 
      age was 10.5 ng/ml (p < .05). For each additional year of age above 1 year of 
      age, AMH concentrations fell by 0.5 ng/ml. There was no effect of AMH 
      concentration on the whelping rate. Smaller breeds had smaller litters (and 
      higher AMH concentrations), but within each breed size category, bitches with 
      higher AMH concentrations had significantly larger litter sizes (p < .01). For 
      each 1 ng/ml increase in AMH, litter size increased by 0.3 pups/litter. In 
      conclusion, we determined a normal reference interval for AMH in bitches based on 
      body weight using a canine-specific assay. In agreement with findings in humans 
      and other species, we found that there is a decline in AMH concentrations with 
      advancing age in bitches. Finally, the significant positive correlation between 
      AMH concentrations and litter size indicates that AMH may be a useful management 
      tool for the selection of bitches in breeding programmes.
CI  - (c) 2016 Blackwell Verlag GmbH.
FAU - Hollinshead, F K
AU  - Hollinshead FK
AD  - Glenbred; Matamata Veterinary Services Ltd, Matamata, New Zealand.
FAU - Walker, C
AU  - Walker C
AD  - New Zealand Veterinary Pathology Ltd, Hamilton, New Zealand.
FAU - Hanlon, D W
AU  - Hanlon DW
AD  - Glenbred; Matamata Veterinary Services Ltd, Matamata, New Zealand.
LA  - eng
PT  - Journal Article
DEP - 20161018
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Biomarkers)
RN  - 80497-65-0 (Anti-Mullerian Hormone)
SB  - IM
MH  - Animals
MH  - Anti-Mullerian Hormone/*blood
MH  - Biomarkers/blood
MH  - Breeding
MH  - Cryopreservation/veterinary
MH  - Dogs
MH  - Female
MH  - Linear Models
MH  - *Litter Size
MH  - Logistic Models
MH  - Multivariate Analysis
MH  - *Ovarian Reserve
MH  - Prospective Studies
MH  - Semen Preservation/veterinary
OTO - NOTNLM
OT  - anti-Mullerian hormone
OT  - artificial insemination
OT  - breeding management
OT  - canine
OT  - ovary
OT  - pre-antral follicles
OT  - primordial follicles
EDAT- 2016/10/21 06:00
MHDA- 2017/09/22 06:00
CRDT- 2016/10/21 06:00
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2017/09/22 06:00 [medline]
PHST- 2016/10/21 06:00 [entrez]
AID - 10.1111/rda.12822 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2017 Apr;52 Suppl 2:35-40. doi: 10.1111/rda.12822. Epub 2016 
      Oct 18.