PMID- 27759829
OWN - NLM
STAT- MEDLINE
DCOM- 20170713
LR  - 20220310
IS  - 2317-6385 (Electronic)
IS  - 1679-4508 (Print)
IS  - 1679-4508 (Linking)
VI  - 14
IP  - 3
DP  - 2016 Jul-Sep
TI  - Analysis of cytokine profile and growth factors in platelet-rich plasma obtained 
      by open systems and commercial columns.
PG  - 391-397
LID - S1679-45082016000300391 [pii]
LID - 10.1590/S1679-45082016AO3548 [doi]
AB  - OBJECTIVE: To evaluate growth factors and cytokines in samples of platelet-rich 
      plasma obtained by three different centrifugation methods. METHODS: Peripheral 
      blood of six individuals with no hematological diseases, aged 18 to 68 years, was 
      drawn to obtain platelet-rich plasma, using the open method and commercial 
      columns by Medtronic and Biomet. The products obtained with the different types 
      of centrifugation were submitted to laboratory analysis, including 
      pro-inflammatory cytokines and chemokines by flow cytometry assays, the 
      concentration of fibroblast growth factors-2 (FGF-2) and transforming growth 
      factor-beta1 (TGF-beta1). RESULTS: The diverse separation methods generated 
      systematically different profiles regarding number of platelets and leukocytes. 
      The Medtronic system yielded a product with the highest concentration of 
      platelets, and the open method, with the lowest concentration of platelets. The 
      results of cytokine analysis showed that the different types of centrifugation 
      yielded products with high concentrations of interleukin 8, interleukin 1beta. The 
      open system resulted in a product with high levels of interleukin 6. Other 
      cytokines and chemokines measured were similar between systems. The product 
      obtained with the open method showed higher levels of TGF-beta1 in relation to other 
      systems and low FGF-2 levels. CONCLUSION: The formed elements, growth factors and 
      cytokines in samples of platelet-rich plasma varied according to the 
      centrifugation technique used. OBJETIVO: Avaliar fatores de crescimento e 
      citocinas em amostras de plasma rico em plaquetas obtidas por tres diferentes 
      metodos de centrifugacao. METODOS: Foi coletado sangue periferico de seis 
      individuos, sem doenca hematologica, com idades entre 18 e 68 anos, para obtencao 
      de plasma rico em plaquetas, utilizando o metodo aberto e sistemas comerciais das 
      empresas Medtronic e Biomet. Os produtos obtidos com os diferentes tipos de 
      centrifugacao foram submetidos as analises laboratoriais, incluindo citocinas 
      proinflamatorias e quimiocinas, por meio de ensaios de citometria de fluxo, 
      concentracao do fator de crescimento fibroblastico-2 (FGF-2) e fator de 
      crescimento transformador-beta1 (TGF-beta1). RESULTADOS: As diferentes 
      centrifugacoes geraram perfis sistematicamente diferentes referentes ao numero de 
      plaquetas e de leucocitos. O sistema da Medtronic originou produto com a maior 
      concentracao de plaquetas, e o metodo aberto com a menor concentracao de 
      plaquetas. Os resultados da analise de citocinas demonstraram que os diferentes 
      tipos de centrifugacao originaram produtos com elevadas concentracoes de 
      interleucina 8 e interleucina 1beta. O sistema aberto resultou em produto com 
      elevados niveis de interleucina 6. As demais citocinas e quimiocinas mensuradas 
      foram similares entre os sistemas. O produto obtido com o metodo aberto 
      apresentou niveis superiores de TGF-beta1 em relacao aos demais sistemas e reduzidos 
      niveis de FGF-2. CONCLUSAO: Os elementos figurados, fatores de crescimento e 
      citocinas, em amostras de plasma rico em plaquetas, variaram conforme a tecnica 
      de centrifugacao utilizada.
FAU - Pochini, Alberto de Castro
AU  - Pochini AC
AD  - Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, 
      Brazil.
FAU - Antonioli, Eliane
AU  - Antonioli E
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Bucci, Daniella Zanetti
AU  - Bucci DZ
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Sardinha, Luiz Roberto
AU  - Sardinha LR
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Andreoli, Carlos Vicente
AU  - Andreoli CV
AD  - Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, 
      Brazil.
FAU - Ferretti, Mario
AU  - Ferretti M
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Ejnisman, Benno
AU  - Ejnisman B
AD  - Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, 
      Brazil.
FAU - Goldberg, Anna Carla
AU  - Goldberg AC
AD  - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
FAU - Cohen, Moises
AU  - Cohen M
AD  - Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, 
      Brazil.
LA  - eng
LA  - por
PT  - Journal Article
PL  - Brazil
TA  - Einstein (Sao Paulo)
JT  - Einstein (Sao Paulo, Brazil)
JID - 101281800
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Centrifugation/methods
MH  - Chemokines/analysis/blood
MH  - Cytokines/*analysis/blood
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*analysis/blood
MH  - Interleukins/analysis/blood
MH  - Middle Aged
MH  - Platelet-Rich Plasma/*chemistry
MH  - Rotator Cuff Injuries/surgery
MH  - Young Adult
PMC - PMC5234752
COIS- none.
EDAT- 2016/10/21 06:00
MHDA- 2017/07/14 06:00
PMCR- 2016/07/01
CRDT- 2016/10/21 06:00
PHST- 2015/10/04 00:00 [received]
PHST- 2016/06/30 00:00 [accepted]
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2016/10/21 06:00 [entrez]
PHST- 2016/07/01 00:00 [pmc-release]
AID - S1679-45082016000300391 [pii]
AID - S1679-45082016AO3548 [pii]
AID - 10.1590/S1679-45082016AO3548 [doi]
PST - ppublish
SO  - Einstein (Sao Paulo). 2016 Jul-Sep;14(3):391-397. doi: 
      10.1590/S1679-45082016AO3548.