PMID- 27760141 OWN - NLM STAT- MEDLINE DCOM- 20170523 LR - 20190202 IS - 1935-2735 (Electronic) IS - 1935-2727 (Print) IS - 1935-2727 (Linking) VI - 10 IP - 10 DP - 2016 Oct TI - Correlation Between HLA-A, B and DRB1 Alleles and Severe Fever with Thrombocytopenia Syndrome. PG - e0005076 LID - 10.1371/journal.pntd.0005076 [doi] LID - e0005076 AB - OBJECTIVE: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever caused by a tick-borne bunyavirus (SFTSV) in East Asian countries. The role of human leukocyte antigen (HLA) in resistance and susceptibility to SFTSV is not known. We investigated the correlation of HLA locus A, B and DRB1 alleles with the occurrence of SFTS. METHODS: A total of 84 confirmed SFTS patients (patient group) and 501 unrelated non-SFTS patients (healthy individuals as control group) from Shandong Province were genotyped by PCR-sequence specific oligonucleotide probe (PCR-SSOP) for HLA-A, B and DRB1 loci.Allele frequency was calculated and compared using chi2 test or the Fisher's exact test. A corrected P value was calculated with a bonferronis correction. Odds Ratio (OR) and 95% confidence intervals (CI) were calculated by Woolf's method. RESULTS: A total of 11 HLA-A, 23 HLA-B and 12 HLA-DRB1 alleles were identified in the patient group, whereas 15 HLA-A, 30 HLA-B and 13 HLA-DRB1 alleles were detected in the control group. The frequencies of A*30 and B*13 in the SFTS patient group were lower than that in the control group (P = 0.0341 and 0.0085, Pc = 0.5115 and 0.252). The ORs of A*30 and B*13 in the SFTS patient group were 0.54 and 0.49, respectively. The frequency of two-locus haplotype A*30-B*13 was lower in the patient group than in the control group(5.59% versus 12.27%, P = 0.037,OR = 0.41, 95%CI = 0.18-0.96) without significance(Pc>0.05). A*30-B*13-DRB1*07 and A*02-B*15-DRB1*04 had strong associations with SFTS resistance and susceptibility respectively (Pc = 0.0412 and 0.0001,OR = 0.43 and 5.07). CONCLUSION: The host HLA class I polymorphism might play an important role with the occurrence of SFTS. Negative associations were observed with HLA-A*30, HLA-B*13 and Haplotype A*30-B*13, although the associations were not statistically significant. A*30-B*13-DRB1*07 had negative correlation with the occurrence of SFTS; in contrast, haplotype A*02-B*15-DRB1*04 was positively correlated with SFTS. FAU - Ding, Shu-Jun AU - Ding SJ AD - Department of Viral Infectious Diseases Control and Prevention, Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, China. FAU - Zhang, Yi AU - Zhang Y AD - HLA Lab,Blood Center of Shandong Province, Jinan, Shandong Province, China. FAU - Zhang, Xiao-Mei AU - Zhang XM AD - Department of Viral Infectious Diseases Control and Prevention, Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, China. FAU - Jiang, Xiao-Lin AU - Jiang XL AD - Department of Viral Infectious Diseases Control and Prevention, Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, China. FAU - Pang, Bo AU - Pang B AD - Department of Viral Infectious Diseases Control and Prevention, Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, China. FAU - Song, Yong-Hong AU - Song YH AD - HLA Lab,Blood Center of Shandong Province, Jinan, Shandong Province, China. FAU - Wang, Jian-Xing AU - Wang JX AD - Department of Viral Infectious Diseases Control and Prevention, Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, China. FAU - Pei, Yao-Wen AU - Pei YW AD - Department of Viral Infectious Diseases Control and Prevention, Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, China. FAU - Zhu, Chuan-Fu AU - Zhu CF AD - HLA Lab,Blood Center of Shandong Province, Jinan, Shandong Province, China. FAU - Wang, Xian-Jun AU - Wang XJ AD - Department of Viral Infectious Diseases Control and Prevention, Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, China. FAU - Yu, Xue-Jie AU - Yu XJ AD - School of Public Health, Shandong University, Jinan, Shandong Province, China. AD - Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America. LA - eng PT - Journal Article DEP - 20161019 PL - United States TA - PLoS Negl Trop Dis JT - PLoS neglected tropical diseases JID - 101291488 RN - 0 (HLA-A Antigens) RN - 0 (HLA-B Antigens) RN - 0 (HLA-DRB1 Chains) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Bunyaviridae Infections/blood/*genetics/virology MH - Female MH - *Gene Frequency MH - *Genetic Predisposition to Disease MH - Genotype MH - HLA-A Antigens/*genetics MH - HLA-B Antigens/*genetics MH - HLA-DRB1 Chains/*genetics MH - Haplotypes MH - Humans MH - Male MH - Middle Aged MH - Odds Ratio MH - *Phlebovirus/isolation & purification MH - Polymerase Chain Reaction MH - Polymorphism, Genetic PMC - PMC5070855 COIS- The authors have declared that no competing interests exist. EDAT- 2016/10/21 06:00 MHDA- 2017/05/24 06:00 PMCR- 2016/10/19 CRDT- 2016/10/21 06:00 PHST- 2016/04/20 00:00 [received] PHST- 2016/09/27 00:00 [accepted] PHST- 2016/10/21 06:00 [pubmed] PHST- 2017/05/24 06:00 [medline] PHST- 2016/10/21 06:00 [entrez] PHST- 2016/10/19 00:00 [pmc-release] AID - PNTD-D-16-00673 [pii] AID - 10.1371/journal.pntd.0005076 [doi] PST - epublish SO - PLoS Negl Trop Dis. 2016 Oct 19;10(10):e0005076. doi: 10.1371/journal.pntd.0005076. eCollection 2016 Oct.