PMID- 27760328
OWN - NLM
STAT- MEDLINE
DCOM- 20171124
LR  - 20231213
IS  - 2211-1247 (Electronic)
VI  - 17
IP  - 4
DP  - 2016 Oct 18
TI  - Cohesin Removal along the Chromosome Arms during the First Meiotic Division 
      Depends on a NEK1-PP1gamma-WAPL Axis in the Mouse.
PG  - 977-986
LID - S2211-1247(16)31315-8 [pii]
LID - 10.1016/j.celrep.2016.09.059 [doi]
AB  - Mammalian NIMA-like kinase-1 (NEK1) is a dual-specificity kinase highly expressed 
      in mouse germ cells during prophase I of meiosis. Loss of NEK1 induces retention 
      of cohesin on chromosomes at meiotic prophase I. Timely deposition and removal of 
      cohesin is essential for accurate chromosome segregation. Two processes regulate 
      cohesin removal: a non-proteolytic mechanism involving WAPL, sororin, and PDS5B 
      and direct cleavage by separase. Here, we demonstrate a role for NEK1 in the 
      regulation of WAPL loading during meiotic prophase I, via an interaction between 
      NEK1 and PDS5B. This regulation of WAPL by NEK1-PDS5B is mediated by protein 
      phosphatase 1 gamma (PP1gamma), which both interacts with and is a phosphotarget of 
      NEK1. Taken together, our results reveal that NEK1 phosphorylates PP1gamma, leading 
      to the dephosphorylation of WAPL, which, in turn, results in its retention on 
      chromosome cores to promote loss of cohesion at the end of prophase I in mammals.
CI  - Copyright (c) 2016 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Brieno-Enriquez, Miguel A
AU  - Brieno-Enriquez MA
AD  - Department of Biomedical Sciences and Center for Reproductive Genomics, Cornell 
      University, Ithaca, NY 14853, USA.
FAU - Moak, Stefannie L
AU  - Moak SL
AD  - Department of Biomedical Sciences and Center for Reproductive Genomics, Cornell 
      University, Ithaca, NY 14853, USA.
FAU - Toledo, Melissa
AU  - Toledo M
AD  - Department of Biomedical Sciences and Center for Reproductive Genomics, Cornell 
      University, Ithaca, NY 14853, USA.
FAU - Filter, Joshua J
AU  - Filter JJ
AD  - Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 
      14853, USA.
FAU - Gray, Stephen
AU  - Gray S
AD  - Department of Biomedical Sciences and Center for Reproductive Genomics, Cornell 
      University, Ithaca, NY 14853, USA.
FAU - Barbero, Jose L
AU  - Barbero JL
AD  - Department of Cellular and Molecular Biology, Laboratory of Chromosomal Dynamics 
      in Meiosis, Centro de Investigaciones Biologicas (CSIC), Madrid 28040, Spain.
FAU - Cohen, Paula E
AU  - Cohen PE
AD  - Department of Biomedical Sciences and Center for Reproductive Genomics, Cornell 
      University, Ithaca, NY 14853, USA. Electronic address: paula.cohen@cornell.edu.
FAU - Holloway, J Kim
AU  - Holloway JK
AD  - Department of Biomedical Sciences and Center for Reproductive Genomics, Cornell 
      University, Ithaca, NY 14853, USA. Electronic address: jkh44@cornell.edu.
LA  - eng
GR  - T32 HD052471/HD/NICHD NIH HHS/United States
GR  - K99 HD090289/HD/NICHD NIH HHS/United States
GR  - R01 GM097263/GM/NIGMS NIH HHS/United States
GR  - P50 HD076210/HD/NICHD NIH HHS/United States
GR  - R00 HD065870/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Proteins)
RN  - 0 (WAPL protein, mouse)
RN  - EC 2.7.11.1 (NIMA-Related Kinase 1)
RN  - EC 2.7.11.1 (Nek1 protein, mouse)
RN  - EC 3.1.3.16 (Protein Phosphatase 1)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/*metabolism
MH  - Chromatids/*metabolism
MH  - Chromosomal Proteins, Non-Histone/*metabolism
MH  - Chromosomes, Mammalian/*metabolism
MH  - Male
MH  - *Meiosis
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Models, Biological
MH  - NIMA-Related Kinase 1/*metabolism
MH  - Phenotype
MH  - Phosphorylation
MH  - Protein Phosphatase 1/*metabolism
MH  - Proteins/*metabolism
MH  - Signal Transduction
MH  - Spermatozoa/metabolism
MH  - Cohesins
PMC - PMC5123770
MID - NIHMS819027
OTO - NOTNLM
OT  - NIMA-like kinase
OT  - WAPL
OT  - cohesin
OT  - meiosis
OT  - mouse
OT  - prophase pathway
EDAT- 2016/10/21 06:00
MHDA- 2017/11/29 06:00
PMCR- 2016/11/25
CRDT- 2016/10/21 06:00
PHST- 2016/04/29 00:00 [received]
PHST- 2016/07/25 00:00 [revised]
PHST- 2016/09/16 00:00 [accepted]
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/10/21 06:00 [entrez]
PHST- 2016/11/25 00:00 [pmc-release]
AID - S2211-1247(16)31315-8 [pii]
AID - 10.1016/j.celrep.2016.09.059 [doi]
PST - ppublish
SO  - Cell Rep. 2016 Oct 18;17(4):977-986. doi: 10.1016/j.celrep.2016.09.059.