PMID- 27762664
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20220321
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 22
IP  - 23-24
DP  - 2016 Dec
TI  - Limited Immunogenicity of Human Induced Pluripotent Stem Cell-Derived Cartilages.
PG  - 1367-1375
AB  - Articular cartilage damage does not spontaneously heal and could ultimately 
      result in a loss of joint function. Damaged cartilage can be repaired with 
      cell/tissue sources that are transplanted, however, autologous chondrocytes are 
      limited in number as a cell source. Induced pluripotent stem cells (iPSCs) are a 
      relatively new and abundant cell source and can be made from the patient, but at 
      a considerable cost. Because cartilage is immunoprivileged tissue, allogeneic 
      cartilages have been transplanted effectively without matching for human 
      leukocyte antigen (HLA), but are difficult to acquire due to scarcity of donors. 
      In this study, we examined the immunogenicity of human iPSC-derived cartilages 
      (hiPS-Carts) in vitro to evaluate whether allogeneic hiPS-Carts can be a new 
      cell/tissue source. The cells in hiPS-Carts expressed limited amounts of major 
      histocompatibility complex (MHC) class I (HLA-ABC) and MHC class II (HLA-DRDQDP). 
      Treatment with interferon gamma (IFNgamma) induced the expression of MHC class I, but not 
      MHC class II in hiPS-Carts. A mixed lymphocyte reaction assay showed that 
      hiPS-Carts stimulated the proliferation of neither T cells nor the activation of 
      NK cells. Furthermore, hiPS-Carts suppressed the proliferation of T cells 
      stimulated with interleukin 2 and phytohemagglutinin (PHA). Together with 
      previously reported findings, these results suggest that hiPS-Carts are no more 
      antigenic than human cartilage. Additionally, in combination with the fact that 
      iPSCs are unlimitedly expandable and thus can supply unlimited amounts of 
      iPS-Carts from even one iPSC line, they suggest that allogeneic hiPS-Carts are a 
      candidate source for transplantation to treat articular cartilage damage.
FAU - Kimura, Takeshi
AU  - Kimura T
AD  - 1 Cell Induction and Regulation Field, Department of Cell Growth and 
      Differentiation, Center for iPS Cell Research and Application, Kyoto University , 
      Kyoto, Japan .
AD  - 2 Department of Pediatrics, Osaka University Graduate School of Medicine , Osaka, 
      Japan .
FAU - Yamashita, Akihiro
AU  - Yamashita A
AD  - 1 Cell Induction and Regulation Field, Department of Cell Growth and 
      Differentiation, Center for iPS Cell Research and Application, Kyoto University , 
      Kyoto, Japan .
FAU - Ozono, Keiichi
AU  - Ozono K
AD  - 2 Department of Pediatrics, Osaka University Graduate School of Medicine , Osaka, 
      Japan .
FAU - Tsumaki, Noriyuki
AU  - Tsumaki N
AD  - 1 Cell Induction and Regulation Field, Department of Cell Growth and 
      Differentiation, Center for iPS Cell Research and Application, Kyoto University , 
      Kyoto, Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161031
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (HLA Antigens)
RN  - 0 (IFNG protein, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - *Bioprosthesis
MH  - *Cartilage, Articular
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*immunology
MH  - Interferon-gamma/*immunology
MH  - Regeneration/immunology
MH  - T-Lymphocytes/*immunology
PMC - PMC5175426
OTO - NOTNLM
OT  - allogeneic transplantation
OT  - articular cartilage
OT  - induced pluripotent stem (iPS) cells
OT  - regeneration
COIS- Statement No competing financial interests exist.
EDAT- 2016/11/01 06:00
MHDA- 2017/11/29 06:00
PMCR- 2016/12/01
CRDT- 2016/11/01 06:00
PHST- 2016/11/01 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/11/01 06:00 [entrez]
PHST- 2016/12/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2016.0189 [pii]
AID - 10.1089/ten.TEA.2016.0189 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2016 Dec;22(23-24):1367-1375. doi: 10.1089/ten.TEA.2016.0189. 
      Epub 2016 Oct 31.