PMID- 27764710
OWN - NLM
STAT- MEDLINE
DCOM- 20170915
LR  - 20220331
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 79
DP  - 2016 Nov
TI  - Prolonged survival effects induced by immature dendritic cells and regulatory T 
      cells in a rat liver transplantation model.
PG  - 92-97
LID - S0161-5890(16)30210-3 [pii]
LID - 10.1016/j.molimm.2016.10.004 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) and regulatory T (Treg) cells are crucial for 
      inducing immune tolerance. However, the suppressive function of infused Treg 
      cells and immature DCs (imDCs) following solid organ transplantation remains 
      unclear. METHODS: ImDCs derived from DA-donor rats and Treg cells isolated from 
      spleens of Lewis rats were prepared. A heterotopic liver transplantation model 
      was established to examine the immune tolerance effects of infusion of 
      Treg-imDCs, imDCs and Treg cells individually. Th1/Th2 cytokines and TRAL were 
      detected by ELISA. The overall rejection grade was assessed and the rejection 
      activity index (RAI) was calculated. TUNEL-positive lymphocytes were detected in 
      the portal area in liver sections. RESULTS: The infusion of Treg-imDCs was more 
      effective than imDCs or Treg cells individually. Moreover, the expression of 
      IL-10 and TGF-beta1 was significantly up-regulated, and IL-12 expression was 
      significantly down-regulated, especially in the Treg-imDCs group. The percentage 
      of TUNEL-positive cells was significantly higher in the Treg cells and imDCs 
      groups. The RAI values in Treg-imDCs group on days 3 and 7 were lower than 
      control, imDCs and Treg cells groups individually (p<0.05). Both TBIL and ALT 
      levels in the Treg-imDCs and imDCs groups were significantly lower than those of 
      the control and Treg cells groups, and serum TRAL levels increased significantly 
      10days after transplantation in the imDC and Treg-imDC groups compared with the 
      control and Treg cells groups (P<0.001). CONCLUSION: These data demonstrated that 
      infusion of Treg cells and/or imDCs induces alloantigen tolerance and prolongs 
      liver allograft survival. The infusion of Treg-imDCs was more effective than 
      imDCs or Treg cells individually. ImDCs synergize with Treg cells in inducing and 
      maintaining the feedback loop between imDCs and Treg cells in vivo.
CI  - Copyright A(c) 2016 Elsevier Ltd. All rights reserved.
FAU - He, Wubing
AU  - He W
AD  - Fujian Provincial Hospital, Provincial Clinical Medical College, Fujian Medical 
      University, Fuzhou, Fujian 350001, China.
FAU - Chen, Lihong
AU  - Chen L
AD  - Department of Pathology, School of Basic Medical Sciences of Fujian Medical 
      University, Fuzhou, Fujian 350004, China; Department of Pathology, Mengchao 
      Hepatobiliary Hospital of Fujian Medical University, Fuzhou, Fujian 350025, 
      China. Electronic address: drlhchen@sina.com.
FAU - Zheng, Lin
AU  - Zheng L
AD  - Department of Pathology, School of Basic Medical Sciences of Fujian Medical 
      University, Fuzhou, Fujian 350004, China.
FAU - Luo, Liuping
AU  - Luo L
AD  - Department of Pathology, Mengchao Hepatobiliary Hospital of Fujian Medical 
      University, Fuzhou, Fujian 350025, China.
FAU - Gao, Lingyun
AU  - Gao L
AD  - Department of Pathology, School of Basic Medical Sciences of Fujian Medical 
      University, Fuzhou, Fujian 350004, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161017
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
SB  - IM
MH  - Allografts
MH  - Animals
MH  - Dendritic Cells/*immunology/transplantation
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Graft Survival/*immunology
MH  - Immune Tolerance/*immunology
MH  - In Situ Nick-End Labeling
MH  - *Liver Transplantation
MH  - Male
MH  - Rats
MH  - Rats, Inbred Lew
MH  - T-Lymphocytes, Regulatory/*immunology/transplantation
OTO - NOTNLM
OT  - Dendritic cells
OT  - Immune tolerance
OT  - Liver transplantation
OT  - Regulatory T cells
EDAT- 2016/10/21 06:00
MHDA- 2017/09/16 06:00
CRDT- 2016/10/21 06:00
PHST- 2016/07/11 00:00 [received]
PHST- 2016/10/09 00:00 [revised]
PHST- 2016/10/12 00:00 [accepted]
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2017/09/16 06:00 [medline]
PHST- 2016/10/21 06:00 [entrez]
AID - S0161-5890(16)30210-3 [pii]
AID - 10.1016/j.molimm.2016.10.004 [doi]
PST - ppublish
SO  - Mol Immunol. 2016 Nov;79:92-97. doi: 10.1016/j.molimm.2016.10.004. Epub 2016 Oct 
      17.