PMID- 27764831
OWN - NLM
STAT- MEDLINE
DCOM- 20170116
LR  - 20170116
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 149
IP  - 4
DP  - 2016
TI  - Mixed-Up Sex Chromosomes: Identification of Sex Chromosomes in the X1X1X2X2/X1X2Y 
      System of the Legless Lizards of the Genus Lialis (Squamata: Gekkota: 
      Pygopodidae).
PG  - 282-289
AB  - Geckos in general show extensive variability in sex determining systems, but only 
      male heterogamety has been demonstrated in the members of their legless family 
      Pygopodidae. In the pioneering study published more than 45 years ago, multiple 
      sex chromosomes of the type X1X1X2X2/X1X2Y were described in Burton's legless 
      lizard (Lialisburtonis) based on conventional cytogenetic techniques. We 
      conducted cytogenetic analyses including comparative genomic hybridization and 
      fluorescence in situ hybridization (FISH) with selected cytogenetic markers in 
      this species and the previously cytogenetically unstudied Papua snake lizard 
      (Lialis jicari) to better understand the nature of these sex chromosomes and 
      their differentiation. Both species possess male heterogamety with an 
      X1X1X2X2/X1X2Y sex chromosome system; however, the Y and one of the X chromosomes 
      are not small chromosomes as previously reported in L. burtonis, but the largest 
      macrochromosomal pair in the karyotype. The Y chromosomes in both species have 
      large heterochromatic blocks with extensive accumulations of GATA and AC 
      microsatellite motifs. FISH with telomeric probe revealed an exclusively terminal 
      position of telomeric sequences in L. jicari (2n = 42 chromosomes in females), 
      but extensive interstitial signals, potentially remnants of chromosomal fusions, 
      in L.burtonis (2n = 34 in females). Our study shows that even largely 
      differentiated and heteromorphic sex chromosomes might be misidentified by 
      conventional cytogenetic analyses and that the application of more sensitive 
      cytogenetic techniques for the identification of sex chromosomes is beneficial 
      even in the classical examples of multiple sex chromosomes.
CI  - (c) 2016 S. Karger AG, Basel.
FAU - Rovatsos, Michail
AU  - Rovatsos M
AD  - Department of Ecology, Faculty of Science, Charles University, Prague, Czech 
      Republic.
FAU - Johnson Pokorna, Martina
AU  - Johnson Pokorna M
FAU - Altmanova, Marie
AU  - Altmanova M
FAU - Kratochvil, Lukas
AU  - Kratochvil L
LA  - eng
PT  - Journal Article
DEP - 20161021
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Animals
MH  - Comparative Genomic Hybridization
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Male
MH  - Microsatellite Repeats/genetics
MH  - Reptiles/*genetics
MH  - Sex Chromosomes/*genetics
MH  - Telomere/genetics
EDAT- 2016/10/21 06:00
MHDA- 2017/01/17 06:00
CRDT- 2016/10/21 06:00
PHST- 2016/07/05 00:00 [accepted]
PHST- 2016/10/21 06:00 [pubmed]
PHST- 2017/01/17 06:00 [medline]
PHST- 2016/10/21 06:00 [entrez]
AID - 000450734 [pii]
AID - 10.1159/000450734 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2016;149(4):282-289. doi: 10.1159/000450734. Epub 2016 Oct 
      21.