PMID- 27765052
OWN - NLM
STAT- MEDLINE
DCOM- 20171010
LR  - 20210109
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 47
IP  - 1
DP  - 2016 Oct 20
TI  - Distinct functional enrichment of transcriptional signatures in pigs with high 
      and low IFN-gamma responses after vaccination with a porcine reproductive and 
      respiratory syndrome virus (PRRSV).
PG  - 104
LID - 104
AB  - Little is known about the host factor in the response to PRRSV vaccination. For 
      this purpose, piglets were immunized with a commercial PRRSV-live vaccine and 
      classified as high responders (HR) or low responders (LR) as regards to the 
      frequencies of virus-specific IFN-gamma-secreting cells. Six weeks post vaccination, 
      PBMCs isolated from three individuals with the most extreme responses in each HR 
      and LR groups and 3 unvaccinated controls, were either stimulated with 
      phytohaemagglutinin, challenged with the vaccine or mock treated for 24 h, prior 
      conducting transcriptional studies, gene ontology and pathway analyses. The LR 
      group had very low neutralizing antibody levels and showed a higher number of 
      down-regulated transcripts compared with the HR group (FDR < 0.2, P < 0.001). 
      Down-regulated genes encoded chemoattractants, proinflammatory cytokines and the 
      interferon-inducible GBP family, and showed enrichment in wounding 
      (FDR < 3.6E-13), inflammation (FDR < 8E-12), defence (FDR < 8.7E-09) and immunity 
      (FDR < 7.6E-08), suggesting immune response impairment. In the HR group, 
      down-regulated genes were involved in protein transport (FDR < 4.77E-03), 
      locomotory behavior (FDR < 5.47E-3), regulation of protein localization 
      (FDR < 1.02E-02), and regulation of TNF superfamily member 15 and miR181. In 
      contrast, the HR group presented up-regulated transcripts associated with 
      wounding (FDR < 4.95). Moreover, IFN-gamma was predicted to be an inhibited upstream 
      regulator since IFN-gamma pathways were associated with higher number of 
      down-regulated genes in the LR (n = 40) than the HR (n = 10). Divergent responses 
      to PRRSV-vaccination may be the result of the genetic background of the host.
FAU - Ait-Ali, Tahar
AU  - Ait-Ali T
AD  - The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of 
      Edinburgh, Midlothian, EH25 9RG, UK.
FAU - Diaz, Ivan
AU  - Diaz I
AD  - Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat 
      Autonoma de Barcelona (UAB), 08193, Cerdanyola del Valles, Spain.
FAU - Soldevila, Ferran
AU  - Soldevila F
AD  - Virology Department, Animal and Plant Health and Agency, Addlestone, KT15 3NB, 
      UK.
AD  - Department of Pathology and Pathogen Biology, Royal Veterinary College, Hatfield, 
      AL9 7TA, UK.
FAU - Cano, Esmeralda
AU  - Cano E
AD  - Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat 
      Autonoma de Barcelona (UAB), 08193, Cerdanyola del Valles, Spain.
FAU - Li, Yanli
AU  - Li Y
AD  - Department Sanitat i Anatomia Animals, Faculty of Veterinary, UAB, 08193, 
      Cerdanyola del Valles, Spain.
FAU - Wilson, Alison D
AU  - Wilson AD
AD  - The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of 
      Edinburgh, Midlothian, EH25 9RG, UK.
FAU - Giotti, Bruno
AU  - Giotti B
AD  - The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of 
      Edinburgh, Midlothian, EH25 9RG, UK.
FAU - Archibald, Alan L
AU  - Archibald AL
AD  - The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of 
      Edinburgh, Midlothian, EH25 9RG, UK.
FAU - Mateu, Enric
AU  - Mateu E
AD  - Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat 
      Autonoma de Barcelona (UAB), 08193, Cerdanyola del Valles, Spain.
AD  - Department Sanitat i Anatomia Animals, Faculty of Veterinary, UAB, 08193, 
      Cerdanyola del Valles, Spain.
FAU - Darwich, Laila
AU  - Darwich L
AD  - Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat 
      Autonoma de Barcelona (UAB), 08193, Cerdanyola del Valles, Spain. 
      laila.darwich@uab.cat.
AD  - Department Sanitat i Anatomia Animals, Faculty of Veterinary, UAB, 08193, 
      Cerdanyola del Valles, Spain. laila.darwich@uab.cat.
LA  - eng
GR  - BBS/E/D/20211552/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161020
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Gene Expression Profiling/*veterinary
MH  - Immunity, Humoral/immunology
MH  - Interferon-gamma Release Tests/*veterinary
MH  - Leukocytes, Mononuclear/immunology
MH  - Oligonucleotide Array Sequence Analysis/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Viral Vaccines/immunology/*pharmacology
PMC - PMC5073823
EDAT- 2016/10/22 06:00
MHDA- 2017/10/11 06:00
PMCR- 2016/10/20
CRDT- 2016/10/22 06:00
PHST- 2016/03/17 00:00 [received]
PHST- 2016/10/05 00:00 [accepted]
PHST- 2016/10/22 06:00 [pubmed]
PHST- 2017/10/11 06:00 [medline]
PHST- 2016/10/22 06:00 [entrez]
PHST- 2016/10/20 00:00 [pmc-release]
AID - 10.1186/s13567-016-0392-3 [pii]
AID - 392 [pii]
AID - 10.1186/s13567-016-0392-3 [doi]
PST - epublish
SO  - Vet Res. 2016 Oct 20;47(1):104. doi: 10.1186/s13567-016-0392-3.