PMID- 27765222
OWN - NLM
STAT- MEDLINE
DCOM- 20161221
LR  - 20181202
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 219
DP  - 2017 Mar 15
TI  - Rationale behind the near-ideal catalysis of Candida antarctica lipase A (CAL-A) 
      for highly concentrating omega-3 polyunsaturated fatty acids into monoacylglycerols.
PG  - 230-239
LID - S0308-8146(16)31548-5 [pii]
LID - 10.1016/j.foodchem.2016.09.149 [doi]
AB  - Dramatic decline in the quality and quantity of omega-3 PUFAs from marine resource 
      demands new environmental-friendly technology to produce high quality omega-3 PUFAs 
      concentrates in a better bioavailable form. Accordingly this work demonstrated an 
      exceptionally highly efficient non-aqueous approach that non-regiospecific and 
      non omega-3 PUFAs preferential Candida antarctica lipase A (CAL-A), functioning as a 
      near-ideal biocatalyst, is capable to directly concentrate omega-3 PUFAs from 20% to 
      30% in oils to up to >90% in monoacylglycerols form through one step reaction. 
      The rationale behind the experimental observation is justified and the catalytic 
      property and specificity of an ideal enzyme tackling this task are defined. High 
      selectivity and efficiency, excellent reusability of biocatalyst, general 
      applicability for concentrating omega-3 PUFAs from both fish and microalgae oils, 
      simple process for product recovery (e.g. by short path distillation), make this 
      novel approach a highly industrially relevant and with potential application in 
      food and drug industries.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - He, Yongjin
AU  - He Y
AD  - Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark; College of Life Science, Fujian Normal University, No. 1, Keji Road, 
      Minhou, Fuzhou 350117, China; Engineering Research Center of Industrial 
      Microbiology of Ministry of Education, Fujian Normal University, No. 1, Keji 
      Road, Minhou, Fuzhou 350117, China.
FAU - Li, Jingbo
AU  - Li J
AD  - Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark.
FAU - Kodali, Sitharam
AU  - Kodali S
AD  - Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark.
FAU - Chen, Bilian
AU  - Chen B
AD  - College of Life Science, Fujian Normal University, No. 1, Keji Road, Minhou, 
      Fuzhou 350117, China; Engineering Research Center of Industrial Microbiology of 
      Ministry of Education, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 
      350117, China. Electronic address: chenbil@fjnu.edu.cn.
FAU - Guo, Zheng
AU  - Guo Z
AD  - Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, 
      Denmark. Electronic address: guo@eng.au.dk.
LA  - eng
PT  - Journal Article
DEP - 20160924
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Fish Oils)
RN  - 0 (Monoglycerides)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Animals
MH  - Candida/classification/*enzymology
MH  - Catalysis
MH  - Distillation
MH  - Fatty Acids, Omega-3/*metabolism
MH  - Fatty Acids, Unsaturated/analysis
MH  - Fish Oils/chemistry/metabolism
MH  - *Industrial Microbiology
MH  - Lipase/*metabolism
MH  - Magnetic Resonance Spectroscopy
MH  - Microalgae/chemistry
MH  - Monoglycerides/*metabolism
OTO - NOTNLM
OT  - Candida antarctica lipase A (CAL-A)
OT  - Docosahexaenoic acid (PubChem CID: 445580)
OT  - Eicosapentaenoic acid (PubChem CID: 446284)
OT  - Ethanol (PubChem CID: 702)
OT  - Ethanolysis
OT  - Fish oil
OT  - Monoacylglcerols (MAGs)
OT  - Oleic acid (PubChem CID: 445639)
OT  - Palmitic acid (PubChem CID: 985)
OT  - Stearidonic acid (PubChem CID: 5312508)
OT  - alpha-Linolenic acid (PubChem CID: 5280934)
OT  - omega-3 Polyunsaturated fatty acids (omega-3 PUFAs)
EDAT- 2016/10/22 06:00
MHDA- 2016/12/22 06:00
CRDT- 2016/10/22 06:00
PHST- 2016/06/09 00:00 [received]
PHST- 2016/08/27 00:00 [revised]
PHST- 2016/09/23 00:00 [accepted]
PHST- 2016/10/22 06:00 [pubmed]
PHST- 2016/12/22 06:00 [medline]
PHST- 2016/10/22 06:00 [entrez]
AID - S0308-8146(16)31548-5 [pii]
AID - 10.1016/j.foodchem.2016.09.149 [doi]
PST - ppublish
SO  - Food Chem. 2017 Mar 15;219:230-239. doi: 10.1016/j.foodchem.2016.09.149. Epub 
      2016 Sep 24.