PMID- 27765649 OWN - NLM STAT- MEDLINE DCOM- 20170213 LR - 20171116 IS - 1532-0456 (Print) IS - 1532-0456 (Linking) VI - 191 DP - 2017 Jan TI - Consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (Oncorhynchus mykiss). PG - 129-137 LID - S1532-0456(16)30130-2 [pii] LID - 10.1016/j.cbpc.2016.10.004 [doi] AB - Rainbow trout (Oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (EMB) and deltamethrin (DM) during outbreaks of Caligus rogercresseyi in a farm located in southern Chile (Hornopiren, Chiloe), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome P450 1A (CYP1A), flavin-containing monooxygenase (FMO) and glutathione S-transferase (GST) in different tissues. Consecutive and alternating EMB/DM treatments resulted in a 10-fold increase and 3-fold decrease of CYP1A protein levels in the intestine and gills, respectively. Notably, CYP1A activity levels decreased in most of the analyzed tissues. FMO protein and activity levels markedly increased in the kidney and the intestine. GST was up-regulated in all tissues, either as protein or enzyme activity. When comparing consecutive EMB/DM treatments against previous studies of EMB treatment alone, CYP1A activity levels were similarly diminished, except in muscle. Likewise, FMO activity levels were increased in most of the analyzed tissues, particularly in the muscle, kidney, and intestine. The increases observed for GST were essentially unchanged between consecutive EMB/DM and EMB only treatments. These results indicate that consecutive EMB/DM treatments in rainbow trout induce the expression and activity of FMO and GST enzymes and decrease CYP1A activity. These altered activities of detoxification enzymes could generate imbalances in metabolic processes, synthesis, degradation of hormones and complications associated with drug interactions. It is especially important when analyzing possible effects of consecutive antiparasitic treatments on withholding periods and salmon farming yields. CI - Copyright A(c) 2016 Elsevier Inc. All rights reserved. FAU - Carcamo, Juan Guillermo AU - Carcamo JG AD - Instituto de Bioquimica y Microbiologia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile; Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Chile. Electronic address: gcarcamo@uach.cl. FAU - Aguilar, Marcelo N AU - Aguilar MN AD - Instituto de Bioquimica y Microbiologia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile. FAU - Carreno, Constanza F AU - Carreno CF AD - Instituto de Bioquimica y Microbiologia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile. FAU - Vera, Tamara AU - Vera T AD - Instituto de Bioquimica y Microbiologia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile; Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Chile. FAU - Arias-Darraz, Luis AU - Arias-Darraz L AD - Instituto de Bioquimica y Microbiologia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile. FAU - Figueroa, Jaime E AU - Figueroa JE AD - Instituto de Bioquimica y Microbiologia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile; Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Chile. FAU - Romero, Alex P AU - Romero AP AD - Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Chile; Laboratorio de Biotecnologia y Patologia Acuatica, Instituto de Patologia Animal, Universidad Austral de Chile, Valdivia, Chile. FAU - Alvarez, Marco AU - Alvarez M AD - Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Chile; Laboratorio de Biologia Celular y Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Quillota 980, Vina del Mar, Chile. FAU - Yanez, Alejandro J AU - Yanez AJ AD - Instituto de Bioquimica y Microbiologia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile; Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Chile. LA - eng PT - Comparative Study PT - Journal Article DEP - 20161017 PL - United States TA - Comp Biochem Physiol C Toxicol Pharmacol JT - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JID - 100959500 RN - 0 (Antiparasitic Agents) RN - 0 (Fish Proteins) RN - 0 (Nitriles) RN - 0 (Pyrethrins) RN - 2JTS8R821G (decamethrin) RN - 70288-86-7 (Ivermectin) RN - EC 1.13.- (Oxygenases) RN - EC 1.14.13.8 (dimethylaniline monooxygenase (N-oxide forming)) RN - EC 1.14.14.1 (Cytochrome P-450 CYP1A1) RN - EC 2.5.1.18 (Glutathione Transferase) RN - HVM3G4A01W (emamectin benzoate) SB - IM MH - Animals MH - Antiparasitic Agents/*toxicity MH - Cytochrome P-450 CYP1A1/genetics/*metabolism MH - Drug Therapy, Combination MH - Fish Diseases/*drug therapy/parasitology MH - Fish Proteins/genetics/*metabolism MH - Gene Expression Regulation, Enzymologic/*drug effects MH - Glutathione Transferase/genetics/*metabolism MH - Inactivation, Metabolic/*drug effects MH - Ivermectin/*analogs & derivatives/toxicity MH - Lice Infestations/drug therapy/parasitology/*veterinary MH - Nitriles/*toxicity MH - Oncorhynchus mykiss/genetics/*metabolism/parasitology MH - Oxygenases/genetics/*metabolism MH - Pyrethrins/*toxicity OTO - NOTNLM OT - Cytochrome P450 OT - Deltamethrin OT - Drug detoxification OT - Emamectin benzoate OT - Flavin-monooxygenase OT - Glutathione S-transferase EDAT- 2016/10/28 06:00 MHDA- 2017/02/14 06:00 CRDT- 2016/10/28 06:00 PHST- 2016/08/09 00:00 [received] PHST- 2016/10/13 00:00 [revised] PHST- 2016/10/13 00:00 [accepted] PHST- 2016/10/28 06:00 [pubmed] PHST- 2017/02/14 06:00 [medline] PHST- 2016/10/28 06:00 [entrez] AID - S1532-0456(16)30130-2 [pii] AID - 10.1016/j.cbpc.2016.10.004 [doi] PST - ppublish SO - Comp Biochem Physiol C Toxicol Pharmacol. 2017 Jan;191:129-137. doi: 10.1016/j.cbpc.2016.10.004. Epub 2016 Oct 17.