PMID- 27768071
OWN - NLM
STAT- MEDLINE
DCOM- 20170801
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 116
DP  - 2016 Oct 9
TI  - A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into 
      Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille 
      Calmette-Guerin.
LID - 10.3791/54620 [doi]
LID - 54620
AB  - Dendritic cells (DCs) are important for initiating immune responses, in part 
      through their ability to acquire and shuttle antigen to the draining lymph node 
      (DLN). The mobilization of DCs to the DLN is complex and remains to be fully 
      elucidated during infection. Herein described is the use of an innovative, simple 
      assay that relies on the fluorochrome 5- and 6-carboxyfluorescein diacetate 
      succinimidyl ester (CFSE) to track the migration of DCs during footpad infection 
      with Mycobacterium bovis Bacille Calmette-Guerin (BCG) in C57BL/6 mice. This 
      assay enables the characterization of skin DC sub-populations that actively 
      relocate to the draining, popliteal LN in response to BCG. This protocol 
      originates from a BCG model where migratory skin DCs were identified by flow 
      cytometry. The assay is amiable to the study and identification of DCs or other 
      cells that home to the popliteal LN after inoculation of microbes, their 
      metabolites or other inflammatory stimuli in the footpad, and consequently to 
      study factors that regulate the migration of these cells.
FAU - Bollampalli, Vishnu Priya
AU  - Bollampalli VP
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet.
FAU - Nylen, Susanne
AU  - Nylen S
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet.
FAU - Rothfuchs, Antonio Gigliotti
AU  - Rothfuchs AG
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet; 
      antonio.rothfuchs@ki.se.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20161009
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Dendritic Cells
MH  - Flow Cytometry
MH  - Langerhans Cells
MH  - *Lymph Nodes
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mycobacterium bovis
MH  - *Tuberculosis, Bovine
PMC - PMC5092184
EDAT- 2016/10/22 06:00
MHDA- 2017/08/02 06:00
PMCR- 2016/10/09
CRDT- 2016/10/22 06:00
PHST- 2016/10/22 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
PHST- 2016/10/22 06:00 [entrez]
PHST- 2016/10/09 00:00 [pmc-release]
AID - 54620 [pii]
AID - 10.3791/54620 [doi]
PST - epublish
SO  - J Vis Exp. 2016 Oct 9;(116):54620. doi: 10.3791/54620.