PMID- 27769713
OWN - NLM
STAT- MEDLINE
DCOM- 20170925
LR  - 20240109
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 439
DP  - 2017 Jan 5
TI  - Androgens modify Wnt agonists/antagonists expression balance in dermal papilla 
      cells preventing hair follicle stem cell differentiation in androgenetic 
      alopecia.
PG  - 26-34
LID - S0303-7207(16)30429-4 [pii]
LID - 10.1016/j.mce.2016.10.018 [doi]
AB  - In androgenetic alopecia, androgens impair dermal papilla-induced hair follicle 
      stem cell (HFSC) differentiation inhibiting Wnt signaling. Wnt 
      agonists/antagonists balance was analyzed after dihydrotestosterone (DHT) 
      stimulation in androgen-sensitive dermal papilla cells (DPC) cultured as 
      spheroids or monolayer. In both culture conditions, DHT stimulation downregulated 
      Wnt5a and Wnt10b mRNA while the Wnt antagonist Dkk-1 was upregulated. Notably, 
      tissue architecture of DPC-spheroids lowers Dkk-1 and enhances Wnt agonists' 
      basal expression; probably contributing to DPC inductivity. The role of Wnt 
      agonists/antagonists as mediators of androgen inhibition of DPC-induced HFSC 
      differentiation was evaluated. Inductive DPC-conditioned medium supplemented with 
      DKK-1 impaired HFSC differentiation mimicking androgens' action. This effect was 
      associated with inactivation of Wnt/beta-catenin pathway in differentiating HFSC by 
      both DPC-conditioned media. Moreover, addition of WNT10b to DPC-medium 
      conditioned with DHT, overcame androgen inhibition of HFSC differentiation. Our 
      results identify DKK1 and WNT10b as paracrine factors which modulate the HFSC 
      differentiation inhibition involved in androgen-driven balding.
CI  - Copyright A(c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Leiros, Gustavo Jose
AU  - Leiros GJ
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein, Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Saladillo 2468, C1440FFX, 
      Ciudad de Buenos Aires, Argentina.
FAU - Ceruti, Julieta Maria
AU  - Ceruti JM
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein, Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Saladillo 2468, C1440FFX, 
      Ciudad de Buenos Aires, Argentina.
FAU - Castellanos, Maria Lia
AU  - Castellanos ML
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein, Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Saladillo 2468, C1440FFX, 
      Ciudad de Buenos Aires, Argentina.
FAU - Kusinsky, Ana Gabriela
AU  - Kusinsky AG
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein, Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Saladillo 2468, C1440FFX, 
      Ciudad de Buenos Aires, Argentina.
FAU - Balana, Maria Eugenia
AU  - Balana ME
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein, Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Saladillo 2468, C1440FFX, 
      Ciudad de Buenos Aires, Argentina. Electronic address: 
      mbalana@fundacioncassara.org.ar.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161018
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Androgens)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Ligands)
RN  - 0 (RNA, Messenger)
RN  - 0 (Wnt Proteins)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Alopecia/genetics/*pathology
MH  - Androgens/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Line
MH  - Culture Media, Conditioned/pharmacology
MH  - Cytoplasm/drug effects/metabolism
MH  - Hair Follicle/*pathology
MH  - Humans
MH  - Ligands
MH  - RNA, Messenger/genetics/metabolism
MH  - Stem Cells/drug effects/metabolism/*pathology
MH  - Wnt Proteins/*agonists/*antagonists & inhibitors
MH  - Wnt Signaling Pathway/drug effects
MH  - beta Catenin/metabolism
OTO - NOTNLM
OT  - Androgenetic alopecia
OT  - Dermal papilla
OT  - Epidermal stem cells
OT  - Epithelial-mesenchymal interactions
OT  - Wnt ligands
EDAT- 2016/10/30 06:00
MHDA- 2017/09/26 06:00
CRDT- 2016/11/05 06:00
PHST- 2016/06/06 00:00 [received]
PHST- 2016/09/27 00:00 [revised]
PHST- 2016/10/17 00:00 [accepted]
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2017/09/26 06:00 [medline]
PHST- 2016/11/05 06:00 [entrez]
AID - S0303-7207(16)30429-4 [pii]
AID - 10.1016/j.mce.2016.10.018 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2017 Jan 5;439:26-34. doi: 10.1016/j.mce.2016.10.018. Epub 
      2016 Oct 18.