PMID- 27773582
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20181113
IS  - 1097-4199 (Electronic)
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 92
IP  - 4
DP  - 2016 Nov 23
TI  - Fast 3D Imaging of Spine, Dendritic, and Neuronal Assemblies in Behaving Animals.
PG  - 723-738
LID - S0896-6273(16)30703-6 [pii]
LID - 10.1016/j.neuron.2016.10.002 [doi]
AB  - Understanding neural computation requires methods such as 3D acousto-optical (AO) 
      scanning that can simultaneously read out neural activity on both the somatic and 
      dendritic scales. AO point scanning can increase measurement speed and 
      signal-to-noise ratio (SNR) by several orders of magnitude, but high optical 
      resolution requires long point-to-point switching time, which limits imaging 
      capability. Here we present a novel technology, 3D DRIFT AO scanning, which can 
      extend each scanning point to small 3D lines, surfaces, or volume elements for 
      flexible and fast imaging of complex structures simultaneously in multiple 
      locations. Our method was demonstrated by fast 3D recording of over 150 dendritic 
      spines with 3D lines, over 100 somata with squares and cubes, or multiple spiny 
      dendritic segments with surface and volume elements, including in behaving 
      animals. Finally, a 4-fold improvement in total excitation efficiency resulted in 
      about 500 x 500 x 650 mum scanning volume with genetically encoded calcium 
      indicators (GECIs).
CI  - Copyright (c) 2016 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Szalay, Gergely
AU  - Szalay G
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary.
FAU - Judak, Linda
AU  - Judak L
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary.
FAU - Katona, Gergely
AU  - Katona G
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary; 
      MTA-PPKE ITK-NAP B-2p Measurement Technology Group, Faculty of Information 
      Technology, Pazmany Peter Catholic University, Budapest 1083, Hungary.
FAU - Ocsai, Katalin
AU  - Ocsai K
AD  - MTA-PPKE ITK-NAP B-2p Measurement Technology Group, Faculty of Information 
      Technology, Pazmany Peter Catholic University, Budapest 1083, Hungary.
FAU - Juhasz, Gabor
AU  - Juhasz G
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary; 
      MTA-PPKE ITK-NAP B-2p Measurement Technology Group, Faculty of Information 
      Technology, Pazmany Peter Catholic University, Budapest 1083, Hungary.
FAU - Veress, Mate
AU  - Veress M
AD  - Department of Atomic Physics, Budapest University of Technology and Economics, 
      Budapest 1111, Hungary.
FAU - Szadai, Zoltan
AU  - Szadai Z
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary; 
      Two-Photon Laboratory, Faculty of Information Technology, Pazmany Peter Catholic 
      University, Budapest 1083, Hungary.
FAU - Feher, Andras
AU  - Feher A
AD  - Department of Atomic Physics, Budapest University of Technology and Economics, 
      Budapest 1111, Hungary.
FAU - Tompa, Tamas
AU  - Tompa T
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary; 
      Two-Photon Laboratory, Faculty of Information Technology, Pazmany Peter Catholic 
      University, Budapest 1083, Hungary.
FAU - Chiovini, Balazs
AU  - Chiovini B
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary; 
      Two-Photon Laboratory, Faculty of Information Technology, Pazmany Peter Catholic 
      University, Budapest 1083, Hungary.
FAU - Maak, Pal
AU  - Maak P
AD  - Department of Atomic Physics, Budapest University of Technology and Economics, 
      Budapest 1111, Hungary.
FAU - Rozsa, Balazs
AU  - Rozsa B
AD  - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of 
      Experimental Medicine, Hungarian Academy of Sciences, Budapest 1083, Hungary; 
      Two-Photon Laboratory, Faculty of Information Technology, Pazmany Peter Catholic 
      University, Budapest 1083, Hungary. Electronic address: rozsabal@koki.hu.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20161020
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
SB  - IM
MH  - Animals
MH  - *Behavior, Animal
MH  - Cell Body/*ultrastructure
MH  - Dendrites/*ultrastructure
MH  - Dendritic Spines/*ultrastructure
MH  - Imaging, Three-Dimensional
MH  - Mice
MH  - Microscopy
MH  - Neurons/ultrastructure
MH  - Optical Imaging/*methods
MH  - Signal-To-Noise Ratio
PMC - PMC5167293
EDAT- 2016/10/25 06:00
MHDA- 2017/07/27 06:00
PMCR- 2016/11/23
CRDT- 2016/10/25 06:00
PHST- 2015/12/06 00:00 [received]
PHST- 2016/07/19 00:00 [revised]
PHST- 2016/09/20 00:00 [accepted]
PHST- 2016/10/25 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2016/10/25 06:00 [entrez]
PHST- 2016/11/23 00:00 [pmc-release]
AID - S0896-6273(16)30703-6 [pii]
AID - 10.1016/j.neuron.2016.10.002 [doi]
PST - ppublish
SO  - Neuron. 2016 Nov 23;92(4):723-738. doi: 10.1016/j.neuron.2016.10.002. Epub 2016 
      Oct 20.