PMID- 27773753
OWN - NLM
STAT- MEDLINE
DCOM- 20170704
LR  - 20170704
IS  - 1096-0295 (Electronic)
IS  - 0273-2300 (Linking)
VI  - 81
DP  - 2016 Nov
TI  - Acute and repeated doses (28 days) oral toxicity study of Vicenin-1, a flavonoid 
      glycoside isolated from fenugreek seeds in laboratory mice.
PG  - 522-531
LID - S0273-2300(16)30302-6 [pii]
LID - 10.1016/j.yrtph.2016.10.013 [doi]
AB  - Vicenin-1 (fenugreek glycoside) has been proven to possess potent 
      anti-inflammatory and anti-oxidant activity. The objective of the present 
      investigation was to determine in-vivo acute and subacute (28-days repeated dose) 
      oral toxicity of Vicenin-1 isolated from fenugreek seed. Vicenin-1 (93%) was 
      isolated from a hydroalcoholic extract of fenugreek seed and characterized using 
      HPLC, TLC, (1)H NMR and (13)C NMR. Acute oral toxicity (AOT) and subacute 
      toxicity studies of Vicenin-1 were carried out according to OECD 425 (up-and-down 
      procedure) and OCED 407 guidelines in Swiss albino mice. In AOT, Vicenin-1 showed 
      10% mortality when administered at a dose of 5000 mg/kg. However, when vicenin-1 
      was administered for at doses of 37.5, 75, or 150 mg/kg 28-days it did not show 
      any mortality at the administered doses. Vicenin-1 (75 mg/kg) did not show 
      observational, behavioral, biochemical or histopathological toxic effects. There 
      were minor alterations in body weight, hematology, and histopathology of mice 
      administered with Vicenin-1 (150 mg/kg), but these changes were within normal 
      laboratory ranges. The highest concentration of Venicin-1 was found in liver 
      (3.46%) followed by lung (0.65%). In conclusion, Vicenin-1 showed median lethal 
      dose (LD(50)) of 4837.5 mg/kg with no-observed-adverse-effect levels (NOAEL) at 
      75 mg/kg and lowest adverse effect levels (LOAEL) at 150 mg/kg for both sexes of 
      mice during AOT and sub-acute toxicity study, respectively.
CI  - Copyright A(c) 2016 Elsevier Inc. All rights reserved.
FAU - Kandhare, Amit D
AU  - Kandhare AD
AD  - Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed 
      University, Erandwane, Paud Road, Pune, 411 038, India.
FAU - Bodhankar, Subhash L
AU  - Bodhankar SL
AD  - Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed 
      University, Erandwane, Paud Road, Pune, 411 038, India. Electronic address: 
      drslbodh@gmail.com.
FAU - Mohan, V
AU  - Mohan V
AD  - Indus Biotech Private Limited, 1, Rahul Residency, Off Salunke Vihar Road, 
      Kondhwa, Pune, 411 048, India.
FAU - Thakurdesai, Prasad A
AU  - Thakurdesai PA
AD  - Indus Biotech Private Limited, 1, Rahul Residency, Off Salunke Vihar Road, 
      Kondhwa, Pune, 411 048, India.
LA  - eng
PT  - Journal Article
DEP - 20161020
PL  - Netherlands
TA  - Regul Toxicol Pharmacol
JT  - Regulatory toxicology and pharmacology : RTP
JID - 8214983
RN  - 0 (Glucosides)
RN  - 0 (Plant Extracts)
RN  - 0 (vicenin)
RN  - 68990-15-8 (fenugreek seed meal)
RN  - 7V515PI7F6 (Apigenin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Apigenin/*administration & dosage/isolation & purification/*toxicity
MH  - Body Weight/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Glucosides/*administration & dosage/isolation & purification/*toxicity
MH  - Liver/*drug effects/pathology
MH  - Lung/*drug effects/pathology
MH  - Male
MH  - Mice
MH  - Plant Extracts/*chemistry
MH  - Survival Rate
MH  - Toxicity Tests, Acute
MH  - Trigonella/*chemistry
OTO - NOTNLM
OT  - Acute toxicity test
OT  - Flavonol glycoside
OT  - LD(50)
OT  - Lowest adverse effect levels
OT  - No-observed-adverse-effect levels
OT  - Subacute toxicity tests
OT  - Vicenin-1
EDAT- 2016/10/30 06:00
MHDA- 2017/07/05 06:00
CRDT- 2016/10/30 06:00
PHST- 2016/03/19 00:00 [received]
PHST- 2016/10/15 00:00 [revised]
PHST- 2016/10/18 00:00 [accepted]
PHST- 2016/10/30 06:00 [pubmed]
PHST- 2017/07/05 06:00 [medline]
PHST- 2016/10/30 06:00 [entrez]
AID - S0273-2300(16)30302-6 [pii]
AID - 10.1016/j.yrtph.2016.10.013 [doi]
PST - ppublish
SO  - Regul Toxicol Pharmacol. 2016 Nov;81:522-531. doi: 10.1016/j.yrtph.2016.10.013. 
      Epub 2016 Oct 20.