PMID- 27774621
OWN - NLM
STAT- MEDLINE
DCOM- 20170621
LR  - 20191210
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 57
IP  - 1
DP  - 2017 Jan
TI  - Evaluation of a new microbeads assay for granulocyte antibody detection.
PG  - 70-81
LID - 10.1111/trf.13878 [doi]
AB  - BACKGROUND: To reduce the risk of transfusion-associated acute lung injury 
      (TRALI), a high number of plasma donors were tested for human leukocyte antigen 
      (HLA) and human neutrophil antigen (HNA) antibodies. For HNA antibody detection, 
      the gold standard is a combination of the granulocyte immunofluorescence test 
      (GIFT) and the granulocyte agglutination test (GAT). However, these tests are not 
      suitable for a high-throughput of samples. STUDY DESIGN AND METHODS: To evaluate 
      the new generation of the LABScreen MULTI assay (One Lambda, Inc.), which has 
      special new beads for all the known HNA specificities, including HNA-3a, 97 sera 
      samples containing well-defined HNA antibodies were used. For background testing, 
      we used 91 samples from plasma donors previously identified by GAT, GIFT, and the 
      monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) 
      assay. RESULTS: Compared with previous tests, the new LABScreen MULTI assay was 
      highly specific for the HNA-1a, HNA-1b, HNA-2, and HNA-3a antibody specificities 
      required to prevent TRALI. Ninety-eight percent of the HNA-1a, HNA-1b, and HNA-2 
      antibodies could be detected as true positive; and 90% of the HNA-3a antibodies 
      were recognized correctly as positive. False-positive reactions were identified 
      in 5.5% of samples that previously tested negative. CONCLUSION: The detection of 
      HNA-3a antibody specificities could be integrated into the new LABScreen MULTI 
      assay; however, we detected only 90%. In addition, we detected further HNA 
      antibodies, such as HNA-1c, HNA-1d, and some HNA-3b and HNA-4a antibodies. The 
      new generation of LABScreen MULTI is a great step toward feasible high-throughput 
      testing for HNA antibodies. Nevertheless, GIFT and GAT remain the gold-standard 
      methods for the differentiation of rare and currently unknown HNA specificities.
CI  - (c) 2016 AABB.
FAU - Schulz, Undine
AU  - Schulz U
AD  - DRK Blood Service North-East, Cottbus, Germany.
FAU - Reil, Angelika
AU  - Reil A
AD  - DRK Blood Service West, Hagen, Germany.
FAU - Kiefel, Volker
AU  - Kiefel V
AD  - Department of Transfusion Medicine, University of Rostock, Rostock, Germany.
FAU - Bux, Jurgen
AU  - Bux J
AD  - Ruhr University Bochum, Bochum, Germany.
FAU - Moog, Rainer
AU  - Moog R
AD  - DRK Blood Service North-East, Cottbus, Germany.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20161023
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Isoantigens)
SB  - IM
MH  - Acute Lung Injury/blood/etiology/prevention & control
MH  - Agglutination Tests/methods
MH  - Antibodies, Monoclonal/chemistry
MH  - Autoantibodies/*blood
MH  - Female
MH  - Fluorescent Antibody Technique, Direct/methods
MH  - Humans
MH  - Isoantigens/*blood
MH  - Male
MH  - *Microspheres
MH  - Transfusion Reaction
EDAT- 2016/10/25 06:00
MHDA- 2017/06/22 06:00
CRDT- 2016/10/25 06:00
PHST- 2016/02/03 00:00 [received]
PHST- 2016/08/02 00:00 [revised]
PHST- 2016/08/19 00:00 [accepted]
PHST- 2016/10/25 06:00 [pubmed]
PHST- 2017/06/22 06:00 [medline]
PHST- 2016/10/25 06:00 [entrez]
AID - 10.1111/trf.13878 [doi]
PST - ppublish
SO  - Transfusion. 2017 Jan;57(1):70-81. doi: 10.1111/trf.13878. Epub 2016 Oct 23.